We narrowed to 10,047 results for: yeast

1. pJK-caCas9-NatMX-Neut5L

   Plasmid

   #89576

   Purpose

   caCas9 integrating vector into the Neut5L locus

   Depositor

   George Church

   Article

   Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-2T-RNAPII-CTD wt #14-HA

   Plasmid

   #160688

   Purpose

   Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

   Depositor

   Jeffry Corden

   Article

   Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

   Insert

   RNA Polymerase II CTD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C terminal domain only

   Promoter

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pV1393

   Plasmid

   #111430

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYX233

   Plasmid

   #163762

   Purpose

   Empty vector for Gal-inducible yeast expression

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Type

   Empty backbone

   Use

   Tags

   Hemagglutinin (HA) tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJZC588

   Plasmid

   #62315

   Purpose

   sgRNA with 2x MS2 (wt+f6) for yeast cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA + 2x MS2 (wt+f6) binding module
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-neo-hApg5-HA

   Plasmid

   #22948

   Depositor

   Noboru Mizushima

   Article

   Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

   Insert

   Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pFBP-2_6.sensor

   Plasmid

   #162800

   Purpose

   Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

   Depositor

   Matthias Heinemann

   Article

   Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

   Insert

   2_6_RNA-device
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p406 – TDH3-Elo3-GFP11-Ura

   Plasmid

   #177734

   Purpose

   ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   Elo3 (ELO3 Budding Yeast)
   

   Use

   Tags

   GFP11 and HDEL

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-FLJ90652

   Plasmid

   #15359

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   FLJ90652 (INO80E Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYES260-sc.eEF2

    Plasmid

    #85119

    Purpose

    yeast expression of EEF2

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3269 pBS Bcl-2

    Plasmid

    #8764

    Depositor

    Stanley Korsmeyer

    Article

    Silverman et al Proc Natl Acad Sci U S A 1990 Dec;87(24)

    Insert

    Bcl-2 (Bcl2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

12. p414GPD-3xFLAG-Nop4 WT

    Plasmid

    #169261

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WT

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 WT (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. BAS292 C terminal SF-GFP kanR

    Plasmid

    #74087

    Purpose

    S.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistance

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    SF-GFP recoded for S.pombe
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pET26b_Nb.AD102

    Plasmid

    #131403

    Purpose

    E. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptor

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody AD102
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS405 SEC66-VAC8-RFP

    Plasmid

    #166843

    Purpose

    Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8, SEC66 Budding Yeast)
    

    Use

    Tags

    RFP and SEC66 transmembrane domain

    Expression

    Yeast

    Mutation

    SEC66 transmembrane domain

    Promoter

    VAC8 promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFBP-4_1.sensor

    Plasmid

    #162844

    Purpose

    Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #4_1

    Depositor

    Matthias Heinemann

    Article

    Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

    Insert

    4_1_RNA-device
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFBP-4_1m.mutant

    Plasmid

    #162869

    Purpose

    Yeast expression of the mutant #4_1m for the RNA-based sensor of fructose-1,6-bisphosphate #4_1

    Depositor

    Matthias Heinemann

    Article

    Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

    Insert

    4_1m_RNA-device
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJKW1813

    Plasmid

    #174003

    Purpose

    Expresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

    Depositor

    Jing-Ke Weng

    Article

    Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

    Inserts

    PpCYP90G4
    ATR1 (ATR1 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ABF2-2EGFP-ter-URA3

    Plasmid

    #184768

    Purpose

    Marker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    ABF2 (ABF2 Budding Yeast)
    

    Use

    Tags

    2EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS406-PHO5-GFP-CERT PH domain

    Plasmid

    #58725

    Purpose

    Expresses GFP-Tagged CERT PH domain in yeast

    Depositor

    Tim Levine

    Article

    Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

    Insert

    collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
    

    Use

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    Promoter

    PHO5

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only