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Fragment1 with TLS2

Plasmid

#196983

Purpose

Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Intermediate fragment for assembly of gRNA-TLS2 construct

Use

Gateway-compatible entry vector

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHyg-AID*-6FLAG

Plasmid

#99519

Purpose

C-terminal AID*-6FLAG degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

6xFLAG

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

Plasmid

#61594

Purpose

A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

D10A, N580A

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-CLIP-tag

Plasmid

#167274

Purpose

Overexpression of CLIP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

CLIP-tag

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRY-HF1-ABE8e

Plasmid

#185670

Purpose

Expresses SpRY-HF1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY-HF1

Use

CRISPR

Expression

Mammalian

Mutation

SpRY-HF1 D10A

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_YES1_SH3

Plasmid

#178492

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

YES1_SH3 (YES1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEY11

Plasmid

#191017

Purpose

Fluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

Ultrapanneuronal

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Dat - EGFP - Tol2

Plasmid

#85418

Purpose

for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene

Depositor

Marc Ekker

Article

Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:

Insert

dopamine transporter (slc6a3 Zebrafish)

Use

Zebrafish transgenesis

Tags

EGFP

Promoter

dat

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only
pPBT-peRNA_GG-Puro

Plasmid

#173220

Purpose

piggyBac transposon containing hU6 promotor and pegRNA cloning site

Depositor

Jacob Giehm Mikkelsen

Article

Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

Inserts

pegRNA cloning cassette
PuroR

Use

Piggybac

Expression

Mammalian

Promoter

EF1-a and hU6

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-SopE

Plasmid

#221327

Purpose

Dox-inducible expression of SopE in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

SopE

Use

Retroviral

Tags

FLAG

Promoter

TRE3GV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)

Plasmid

#71720

Purpose

Expressing an mRuby-tagged TIR1 in the soma of C. elegans

Depositor

Abby Dernburg

Article

Zhang et al Development. 2015 Nov 9. pii: dev.129635

Insert

TRANSPORT INHIBITOR RESPONSE 1 (TIR1 Mustard Weed)

Tags

mRuby

Expression

Worm

Mutation

codon-optimized for expression in C. elegans; Two…

Promoter

eef-1A.1 (eft-3)

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLL7.0: Venus-iLID-Mito (From ActA)

Plasmid

#60413

Purpose

Mammalian Expression of Venus-iLID-Mito

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-iLID-Mito

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag

Plasmid

#167269

Purpose

Overexpression of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLL7.0: Venus-iLID-CAAX (from KRas4B)

Plasmid

#60411

Purpose

Mammalian Expression of Venus-iLID-CAAX

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-iLID-CAAX

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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CFP-LacI

Plasmid

#238277

Purpose

For overexpression of CFP-LacI

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CFP-LacI

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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NHA-Ago1

Plasmid

#21535

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago1 (AGO1 Human)

Tags

NHA

Expression

Mammalian

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
GW1-rogRFP2

Plasmid

#207876

Purpose

Mammalian expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD8alpha variant 2

Plasmid

#188362

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pGal4BD-CRY2

Plasmid

#28243

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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HIV-dual-GT

Plasmid

#122696

Purpose

Dual-fluorescence reporter to monitor the expression of HIV-1 early genes and late genes simultaneously.

Depositor

Klaus Überla

Article

Xiao et al mBio. 2021 Jan 19;12(1). pii: mBio.02525

Inserts

mTagBFP2
DsRed-max

Use

Lentiviral

Tags

PEST domain

Promoter

HIV-1 LTR

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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