We narrowed to 19,203 results for: comp

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mSIRT3-S

Plasmid

#33310

Depositor

Michael Sack

Article

Bao et al J Cell Biochem. 2010 May;110(1):238-47.

Insert

Sirt3 (Sirt3 Mouse)

Tags

3XFlag

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2

Plasmid

#79902

Purpose

Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

3xFLAG_Twin_Strep_EZH2

Use

CRISPR and TALEN

Tags

3xFLAG_Twin_Strep

Expression

Mammalian

Promoter

PGK1

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pShuttle-mCherry-EB1-C

Plasmid

#37829

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 (MAPRE1 Human)

Use

Adenoviral

Tags

mCherry

Expression

Mammalian

Mutation

aa165-268 only

Promoter

CMV

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only

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HBT_pcoCASphi_version2

Plasmid

#197932

Purpose

Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Tags

2xNLS-2xFLAG

Expression

Plant

Promoter

35SPPDK promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAW-AlphaLobe

Plasmid

#113255

Purpose

E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

Depositor

Jennifer Doudna

Article

Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

S. pyogenes Cas9 alpha-helical lobe

Tags

10xHis-MBP

Expression

Bacterial

Mutation

residues 56–714; See paper for description

Promoter

T7

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-Sp dCas9-mVenusN

Plasmid

#191396

Purpose

SpdCas9 fused to N-terminal part of mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

N-terminal part of split mVenus

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0386 pcmv-hudiscas7-11-s1006-gggs-d1221

Plasmid

#186983

Purpose

mammalian expression of cas7-11-s1006-gggs-d1221

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122

Expression

Mammalian

Mutation

s1006-gggs-d1221 deletion

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBT276

Plasmid

#122607

Purpose

express CDA1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

CDA1-BE4max

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_TRIM25_G1

Plasmid

#127119

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA TRIM25 (TRIM25 Human)

Use

CRISPR

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-CAG.Pro

Plasmid

#79490

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CAG Promoter

Use

Multisite gateway entry clone

Promoter

CAG Promoter

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAd-Track SIRT1

Plasmid

#8440

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Expression

Mammalian

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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GFP-NPM NESM

Plasmid

#13282

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L100A, L102A

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag-MBD1-14-mVenusC

Plasmid

#191393

Purpose

MBD domain of MBD1 fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

pcDNA3 Flag-MBD1-14-mVenusC

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pOPS0750

Plasmid

#115511

Purpose

Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

gusA pOGG083, T-pharma pOGG003 assembled in pOGG026

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Constitutive promoter pNeo pOGG001

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK SCN1B 3'UTR

Plasmid

#19855

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

SCN1B 3'UTR (SCN1B Human)

Expression

Mammalian

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pGUS (GB0208)

Plasmid

#68203

Purpose

Provides the CDS of the β-glucuronidase gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

GUS coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pUCBB-ctH6-eGFP

Plasmid

#32562

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Promoter

Consitutive lac

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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RanBP2-RB3-4-pET23a

Plasmid

#85769

Purpose

bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

Depositor

Frauke Melchior

Article

Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

Insert

RanBP2 fragment (RANBP2 Human)

Tags

His

Expression

Bacterial

Mutation

fragment (amino acids 2304-3062)

Promoter

T7

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherry

Plasmid

#182560

Purpose

Expresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherry

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a ICL1
sfGFP-P2A-mCherry

Expression

Mammalian

Promoter

Syn

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-N1-PHPLCd1

Plasmid

#202679

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

PLCD1v2(1-170):PAmCherry1 (PLCD1 Human)

Expression

Mammalian

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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