We narrowed to 6,763 results for: poly
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Plasmid#62919PurposeAAV vector backbone designed to express GFP from a RSV promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVPromoterRous Sarcoma Virus(RSV)Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Hypa-nCas9n-A69
Plasmid#125645Purposemicro-injection of poly-adenylated Hypa-nCas9n RNADepositorInsertHypa-nCas9n
UseRna in vitro transcription vectorMutationN692A, M694A, Q695A, H698A from wild-type Cas9PromoterT3Available SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(R37P)-RagC
Plasmid#99706PurposeBacterial expression of RagA(R37P)-RagCDepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationR37PPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(L91P)
Plasmid#99707PurposeBacterial expression of RagA-RagC(L91P)DepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationL91PPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(K84T)
Plasmid#99708PurposeBacterial expression of RagA-RagC(K84T)DepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationK84TPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(T21N)-RagC
Plasmid#99650PurposeBacterial expression of RagA(T21N)-RagCDepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationT21NAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(S75N)
Plasmid#99699PurposeBacterial expression of RagA-RagC(S75N)DepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationS75NAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(Q66L)-RagC(Q120L)
Plasmid#99702PurposeBacterial expression of RagA(Q66L)-RagC(Q120L)DepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationQ120L and Q66LPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHCMM1
Plasmid#167452PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoterDepositorInsertPTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
ExpressionYeastPromoterTEF1AvailabilityAcademic Institutions and Nonprofits only -
pHCMM2
Plasmid#167453PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoterDepositorInsertPSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
ExpressionYeastPromoterPSEC63AvailabilityAcademic Institutions and Nonprofits only -
c-Flag pcDNA3
Plasmid#20011PurposeThis pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.DepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsFlagExpressionMammalianAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pB_tetO7_sfGFP
Plasmid#196616PurposePlasmid for integrating tetO7-controlled sfGFP into the HO region in yeastDepositorInsertSuperfolder GFP
ExpressionYeastPromotertetO7 promoterAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PARP2-Flag-His-WT
Plasmid#111574PurposeExpresses full length PARP2 in Sf9 (insect) cellsDepositorInsertPARP2 (PARP2 Human)
TagsC-terminal 6xHis and C-terminal FlagExpressionInsectPromoterpolyhedrin promoterAvailable SinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
(SIM)10
Plasmid#126946PurposeBacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.DepositorInsert10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
ExpressionBacterialPromoterunknownAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM
Plasmid#183150PurposeExpression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM)DepositorInsertsLex-ER-B112
Zif268 DBD - hPRLBD - MSN2AD
UseFor yeast genomic integrationAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pscAluYNF1
Plasmid#50934PurposeExpresses scAluY RNA in mammalian cells.DepositorInsertscAluYNF1
ExpressionMammalianMutationthe poly(A) linker and the right arm of the dimer…Promoter7SL RNA promoter (pol III)Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUWR-Cad-Venus
Plasmid#58328PurposeDestination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genomeDepositorInsertCad-Venus
ExpressionInsectPromoterpoly-ubiquitinAvailable SinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYFP-NEAT1m_v1-RT
Plasmid#97089PurposeEncodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.DepositorAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYFP-NEAT1_IS_RT
Plasmid#97091PurposeEncodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.DepositorAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAMV:nAChRgamma-eGFP
Plasmid#29693DepositorInsertGFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)
TagsAMV promotor and poly(A) tailExpressionBacterialMutationeGFP inserted after position S380Available SinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUWR-CadTS
Plasmid#58325PurposeDestination vector for inserting ubi-CadTS to Drosophila melanogaster genomeDepositorInsertE-cadherin tension sensor
ExpressionInsectPromoterpoly-ubiquitinAvailable SinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUWR-Cad-mTFP
Plasmid#58327PurposeDestination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genomeDepositorInsertCad-mTFP
ExpressionInsectPromoterpoly-ubiquitinAvailable SinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-C-TEV-HAT
Plasmid#202597PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianPromoterCAGAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-N-TEV-HAT
Plasmid#202598PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianPromoterCAGAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2A K189-192A IRES GFP
Plasmid#133823PurposeEncodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPMutationK189-192APromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pHCMM3
Plasmid#167454PurposeA control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]DepositorInsertPSEC63_yeGFP_cePUP-2_URA3_TADH1
ExpressionYeastPromoterPSEC63AvailabilityAcademic Institutions and Nonprofits only -
pLV.PARP1#1
Plasmid#14544DepositorAvailable SinceMarch 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLV.PARP1#2
Plasmid#14545DepositorAvailable SinceMarch 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pG2J
Plasmid#102882PurposeExpresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertsOsTIR1
mGFP-AID
TagsAID(71-114)ExpressionYeastPromoterADH1 and TEF1Available SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK331
Plasmid#59572PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP(16) 2xK-turn Kozak EGFP attB2 AscI
Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only