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We narrowed to 2,326 results for: dcas9

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Showing: 1241 - 1280 of 2326 results

  1. THI VP64 pALD4 T1

    Plasmid
    #161482
    Purpose
    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T1_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (targeting T1 of pTHI11 promoter)
    Expression
    Yeast
    Promoter
    pALD4, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161482-plasmid-map-sequence-id-324352

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  2. THI VP64 pALD4 T2

    Plasmid
    #161483
    Purpose
    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T2_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (targeting T2 of pTHI11 promoter)
    Expression
    Yeast
    Promoter
    pALD4, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161483-plasmid-map-sequence-id-324353

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  3. THI VP64 pALD4 T3

    Plasmid
    #161484
    Purpose
    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T3_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (targeting T3 of pTHI11 promoter)
    Expression
    Yeast
    Promoter
    pALD4, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161484-plasmid-map-sequence-id-324354

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  5. pGGE-AtREF6

    Plasmid
    #246813
    Purpose
    A GreenGate entry vector containing coding region of AtREF6
    Depositor
    Danhua Jiang
    Insert
    AtREF6
    Use
    Greengate cloning entry vector
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246813-plasmid-map-sequence-id-494308

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  6. pGGE-AtJMJ14

    Plasmid
    #246811
    Purpose
    A GreenGate entry vector containing coding region of AtJMJ14
    Depositor
    Danhua Jiang
    Insert
    AtJMJ14
    Use
    Greengate cloning entry vector
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246811-plasmid-map-sequence-id-494297

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  7. LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

    Plasmid
    #100944
    Purpose
    Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag
    Depositor
    Ryan Lister
    Article
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Synthetic, Human)
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    F636A, E660A, E725A, R295A
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100944-plasmid-map-sequence-id-206112

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  9. TetO-FUW-VdC9BV

    Plasmid
    #62195
    Purpose
    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes
    Depositor
    Kam Leong
    Article
    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7
    Insert
    VP64dCas9BFPVP64
    Use
    Lentiviral
    Tags
    Two VP64s tagged to dCas9 fused to BFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive) Cas9 (dCas9)
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62195-plasmid-map-sequence-id-101643

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  10. pLC133

    Plasmid
    #198639
    Purpose
    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.
    Depositor
    David Bikard
    Article
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    dCas9
    Tags
    3x-FLAG
    Expression
    Bacterial
    Promoter
    pTet
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198639-plasmid-map-sequence-id-390787

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  11. pAPH021

    Plasmid
    #234346
    Purpose
    Library-scale IPTG-inducible single transcript dual-gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK
    Depositor
    Jerome Fox
    Article
    Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12
    Inserts
    Type II dCas9 sgRNA
    Type II dCas9 sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    lacUV5
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234346-plasmid-map-sequence-id-470058

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  12. THI ONLY pALD4 T-

    Plasmid
    #161497
    Purpose
    Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    Expression
    Yeast
    Promoter
    pALD4, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161497-plasmid-map-sequence-id-326273

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  13. pJK434

    Plasmid
    #155378
    Purpose
    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available Since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    155378-plasmid-map-sequence-id-324490

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  14. pGGE-NtDRMcd

    Plasmid
    #246817
    Purpose
    A GreenGate entry vector containing coding region of Nicotiana tabacum DRM methyltransferase catalytic domain
    Depositor
    Danhua Jiang
    Insert
    NtDRMcd
    Use
    Greengate cloning entry vector
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    246817-plasmid-map-sequence-id-494258

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  15. pGGE-AtATXR5set

    Plasmid
    #246805
    Purpose
    A GreenGate entry vector containing coding region of SET domain of AtATXR5
    Depositor
    Danhua Jiang
    Insert
    AtATXR5set
    Use
    Greengate cloning entry vector
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    246805-plasmid-map-sequence-id-495597

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  16. pGGE-AtATX1set

    Plasmid
    #246804
    Purpose
    A GreenGate entry vector containing coding region of SET domain of AtATX1
    Depositor
    Danhua Jiang
    Insert
    AtATX1set
    Use
    Greengate cloning entry vector
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246804-plasmid-map-sequence-id-494331

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  17. pGGE-AtREF6jmj

    Plasmid
    #246814
    Purpose
    A GreenGate entry vector containing coding region of jmj domain of AtREF6
    Depositor
    Danhua Jiang
    Insert
    AtREF6jmj
    Use
    Greengate cloning entry vector
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246814-plasmid-map-sequence-id-494316

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  18. pGGE-AtREF6ΔZnF

    Plasmid
    #246815
    Purpose
    A GreenGate entry vector containing coding region of AtREF6 without zinc finger domain
    Depositor
    Danhua Jiang
    Insert
    AtREF6_ZnF
    Use
    Greengate cloning entry vector
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246815-plasmid-map-sequence-id-494295

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  19. pGGC-ZmUBI Promoter-Insulator

    Plasmid
    #246800
    Purpose
    A GreenGate entry vector containing a ZmUBI promoter and an Insulator
    Depositor
    Danhua Jiang
    Insert
    ZmUBI Promoter-Insulator
    Use
    Greengate cloning entry vector
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246800-plasmid-map-sequence-id-494255

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  20. pGGC-AtUBQ10 promoter-Insulator

    Plasmid
    #246799
    Purpose
    A GreenGate entry vector containing a AtUBQ10 promoter and an Insulator
    Depositor
    Danhua Jiang
    Insert
    AtUBQ10 promoter-Insulator
    Use
    Greengate cloning entry vector
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246799-plasmid-map-sequence-id-494302

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  21. pdCRISPomyces-2BD

    Plasmid
    #234429
    Purpose
    Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified dCas9(D10A, H840A) with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234429-plasmid-map-sequence-id-468849

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  22. pCRISPathBrick

    Plasmid
    #65006
    Purpose
    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive native promoters
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65006-plasmid-map-sequence-id-109903

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  24. pGGD-ZmUBI Promoter-scFV-sfGFP

    Plasmid
    #246802
    Purpose
    A GreenGate entry vector containing scFV-sfGFP fusion gene drived by an ZmUBI Promoter
    Depositor
    Danhua Jiang
    Insert
    ZmUBI Promoter-scFV-sfGFP
    Use
    Greengate cloning entry vector
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    246802-plasmid-map-sequence-id-494247

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  25. lenti dCAS-VP64_Blast

    Plasmid
    #61425
    Purpose
    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Lentiviral Prep
    Insert
    dCAS9(D10A, N863A)-VP64_2A_Blast
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available Since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    61425-plasmid-map-sequence-id-457486

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  30. pPPC011

    Plasmid
    #171143
    Purpose
    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available Since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171143-plasmid-map-sequence-id-345216

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  31. pPPC001

    Plasmid
    #171138
    Purpose
    For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    171138-plasmid-map-sequence-id-344761

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  32. pGGD-AtUBQ10 Promoter-scFV-sfGFP

    Plasmid
    #246803
    Purpose
    A GreenGate entry vector containing scFV-sfGFP fusion gene drived by an AtUBQ10 Promoter
    Depositor
    Danhua Jiang
    Insert
    AtUBQ10 Promoter-scFV-sfGFP
    Use
    Greengate cloning entry vector
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    246803-plasmid-map-sequence-id-494254

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  33. pGGF-NLS-NOS terminator-Hygromycin resistance

    Plasmid
    #246819
    Purpose
    A GreenGate entry vector containing SV40 NLS and Rex NLS followed by a Hygromycin resistance expression cassette
    Depositor
    Danhua Jiang
    Insert
    NLS-NOS terminator-Hygromycin resistance
    Use
    Greengate cloning entry vector
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    246819-plasmid-map-sequence-id-494251

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  34. pGGE-AtFIE

    Plasmid
    #246808
    Purpose
    A GreenGate entry vector containing coding region of AtFIE
    Depositor
    Danhua Jiang
    Insert
    AtFIE (FIE Mustard Weed)
    Use
    Greengate cloning entry vector
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246808-plasmid-map-sequence-id-494301

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  35. pGGD-AtSUC2 Promoter-scFV-sfGFP

    Plasmid
    #246801
    Purpose
    A GreenGate entry vector containing scFV-sfGFP fusion gene drived by an AtSUC2 Promoter
    Depositor
    Danhua Jiang
    Insert
    AtSUC2 Promoter-scFV-sfGFP
    Use
    Greengate cloning entry vector
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246801-plasmid-map-sequence-id-494245

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Showing: 1241 - 1280 of 2326 results