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Showing: 1241 - 1280 of 1911 results
  1. pTRE3G-Bi-gePSI-pGFAP-AcGFP

    Plasmid
    #133733
    Purpose
    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a GFAP promoter.
    Depositor
    Inserts
    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    Use
    AAV
    Tags
    murine ornithine decarboxylase - degron (ODC36)
    Expression
    Mammalian
    Promoter
    pGFAP and pTRE3G-Bi
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. hGFAP-GFP

    Plasmid
    #40592
    Depositor
    Insert
    GFP-S65T
    Use
    Mouse Targeting
    Tags
    MP-1 fragment (mouse protamine 1 intron and polya…
    Expression
    Bacterial and Mammalian
    Mutation
    Construct contains a mutated, humanized hGFP(S65T…
    Promoter
    hGFAP promoter
    Available Since
    Oct. 4, 2012
    Availability
    Academic Institutions and Nonprofits only
  3. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid
    #225139
    Purpose
    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.
    Depositor
    Insert
    Ag43 protein fused to silaffin-R5 and sfGFP
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid
    #185791
    Purpose
    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker
    Depositor
    Insert
    RBP-sfGFP(S45C)-ZdK1
    Tags
    10xHis, TEV protease cleavage site after His Tag
    Expression
    Bacterial
    Mutation
    Ser to Cys mutation at start of GFP, residue 45 i…
    Promoter
    T7
    Available Since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pCS2+ cMyc Dam_f_GFPoNLS

    Plasmid
    #85820
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Insert
    cMyc Dam_f_GFPoNLS
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A.
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. p1.1-Tr2-eGFP

    Plasmid
    #162782
    Purpose
    Fluorescent reporter for protein expression studies
    Depositor
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pCS2+ oDam_f_GFPoNLS

    Plasmid
    #85819
    Purpose
    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Insert
    oDam_f_GFPoNLS
    Tags
    oNLS (optimized nuclear localization signal) C te…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pRD397

    Plasmid
    #163104
    Purpose
    Expression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Insert
    NLS_mTurquoise2_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pAS_4xG1 PylT A41AA C55A sfGFP 150TAG

    Plasmid
    #154770
    Purpose
    plasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Insert
    sfGFP
    Expression
    Mammalian
    Mutation
    150TAG in GFP reporter, G1 PylT with A41AA and C5…
    Promoter
    EF1
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)

    Plasmid
    #127636
    Purpose
    Encodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)
    Depositor
    Insert
    mTurquoiuse2-Tat, YPet-PCP
    Use
    Synthetic Biology
    Tags
    RNA binding peptide: PCP and RNA binding peptide:…
    Expression
    Bacterial
    Available Since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. AAV-tsC2Pro-GFP

    Plasmid
    #248521
    Purpose
    This vector expresses Green Fluorescent Protein under the control of the Tree Shrew Cerebellin 2 Promoter.
    Depositor
    Insert
    Tree Shrew Cerebellin 2 Promoter + Enhanced Green Fluorescent Protein
    Use
    AAV
    Expression
    Mammalian
    Promoter
    Tree Shrew Cerebellin 2 Promoter (tsC2Pro)
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
  12. pAvA143

    Plasmid
    #248147
    Purpose
    mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutations
    Depositor
    Insert
    luxR (gen3 mutations)
    Use
    Integration vector
    Tags
    Gal4 activation domain and nuclear localization s…
    Expression
    Yeast
    Mutation
    Gal4_AD: F51 (TTC→TTT), G88E. LuxR: K53E, N86Y, I…
    Promoter
    pPGK1
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
  13. pAvA141

    Plasmid
    #248146
    Purpose
    mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutations
    Depositor
    Insert
    luxR (gen2 mutations)
    Use
    Integration vector
    Tags
    Gal4 activation domain and nuclear localization s…
    Expression
    Yeast
    Mutation
    Gal4_AD: G8V. NLS: K120N. LuxR: N86K, K104E, M135V
    Promoter
    pPGK1
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
  14. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid
    #227645
    Purpose
    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.
    Depositor
    Insert
    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    Use
    Synthetic Biology
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 1241 - 1280 of 1911 results