We narrowed to 6,177 results for: nls

1. spCas9-NLS-MAV

   Plasmid

   #124212

   Purpose

   SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

   Depositor

   Uri David Akavia

   Article

   Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   His6, MBP, TEV Cleavage site, and two copies of S…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LbCas12a-2C-NLS in pCSDest

   Plasmid

   #126638

   Purpose

   Expresses LbCas12a-2C-NLS in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   LbCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Promoter

   CMV IE94 promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 459 p3E-2A-nlsEGFPpA

   Plasmid

   #195969

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-nlsEGFP (nuclear)
   

   Use

   Other

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNLS-LEXY (pDN138)

   Plasmid

   #72657

   Purpose

   golden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS)

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

   Type

   Empty backbone

   Use

   Golden gate entry vector

   Tags

   NLS and mCherry-LEXY

   Expression

   Mammalian

   Promoter

   CMV-F

   Available Since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX.(NLS)iGlucoSnFR.H66A.L276V

   Plasmid

   #238944

   Purpose

   Green glucose sensor, high sensitivity, nuclear

   Depositor

   Paul Khavari

   Article

   Lopez-Pajares et al Cell Stem Cell. 2025 Mar 20:S1934-5909(2

   Insert

   iGlucoSnFR.L276V.K312A
   

   Use

   Lentiviral

   Tags

   Myc and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX.(NLS)iGlucoSnFR.L276V.K312A

   Plasmid

   #238945

   Purpose

   Green glucose sensor, moderate sensitivity, nuclear

   Depositor

   Paul Khavari

   Article

   Lopez-Pajares et al Cell Stem Cell. 2025 Mar 20:S1934-5909(2

   Insert

   iGlucoSnFR.L276V.K312A
   

   Use

   Lentiviral

   Tags

   Myc and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAd-PV-NLS-DsRed

   Plasmid

   #17287

   Depositor

   Anton Bennett

   Article

   Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

   Insert

   PV-DsRed-NLS-CD (Pvalb Rat)
   

   Use

   Adenoviral

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Feb. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pHAGE-TO-MCP-3XBFPnls

   Plasmid

   #75383

   Purpose

   MCP-3XBFPnls

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   MCP
   

   Use

   Lentiviral

   Tags

   3XBFP

   Expression

   Mammalian

   Promoter

   CMV-TO

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-BiNLS-fYap

   Plasmid

   #176623

   Purpose

   expresses optofYap in zebrafish

   Depositor

   Timothy Saunders

   Article

   Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

   Insert

   Yes associated protein (yap1 Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   mCherry

   Promoter

   SP6

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTol2CG2-QUAS5x:GFPNLS-SV40pA

    Plasmid

    #155122

    Purpose

    Tol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUAS5x:GFPNLS
    

    Use

    Zebrafish expression

    Promoter

    QUAS5x-E1b

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-NLS-M-MLV-RT

    Plasmid

    #181804

    Purpose

    pET28a-NLS-M-MLV RT

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

    Insert

    NLS-M-MLV-RT
    

    Expression

    Bacterial

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Prab-3::NLS::GCaMP6s

    Plasmid

    #68119

    Purpose

    Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    rab-3::NLS::GCaMP6s
    

    Expression

    Worm

    Promoter

    rab-3

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. GANES-DEVD-BNLS

    Plasmid

    #50842

    Purpose

    Expresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddGFP B
    

    Tags

    NES sequence LALKLAGLDIGS placed after ddGFP A an…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mApple-PCNA-19-NLS-4

    Plasmid

    #54937

    Purpose

    Localization: Nucleus, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    PCNA-19 NLS (PCNA Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCSIIbsr-EKARrEV-NLS

    Plasmid

    #173854

    Purpose

    A lentiviral vector for EKARrEV-NLS

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    EKARrEV-NLS
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-His:CRY2:nls:VPR

    Plasmid

    #135989

    Purpose

    Expresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Inserts

    CRY2 domain fused to the VPR activation domain
    CRY2 domain fused to the VPR activation domain
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. NLS-4LNDD-pcDNA3.1(+)

    Plasmid

    #172313

    Purpose

    Expresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cells

    Depositor

    Joshua Rosenthal

    Article

    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain
    

    Tags

    6xHis, Flag, and Nuclear Localization Signal of S…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. epB_CAG_TetRFlag-nls-tdTom_DEx4

    Plasmid

    #119909

    Purpose

    ePiggyBac mammalian expression plasmid for TetR-tdTom fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    TetRFlag-nls-tdTom
    

    Expression

    Mammalian

    Available Since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEN017-SpCas9-hiNLS-t-M4

    Plasmid

    #234455

    Purpose

    Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVITRO1-TagRFP-NLS

    Plasmid

    #184446

    Purpose

    Nucleus-targeted TagRFP expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    TagRFP-3xNLS
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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