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C21orf59_pCSdest

Plasmid

#53798

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

C21orf59 (C21orf59 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1-ccdB-3xHA

Plasmid

#214986

Purpose

Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xHA tag

Depositor

Wade Harper

Type

Empty backbone

Tags

3xHA

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

Plasmid

#191797

Purpose

vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

Insert

humanized dCas13d

Use

CRISPR

Expression

Mammalian

Mutation

R239A, H244A, R858A, H863A

Promoter

CBh, SV40, hU6

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTH100

Plasmid

#84458

Purpose

pJB38-NWMN29-30 + SarA_P1-sGFP-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-sGFP from pCM29

Tags

6xHis (on sfGFP)

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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U6-pegRNA-H1-nick sgRNA-mCherry

Plasmid

#176901

Purpose

Expresses prime editing guide RNA and guide RNA in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

mCherry
gRNA scaffold
gRNA scaffold

Use

AAV

Expression

Mammalian

Promoter

CAG, H1, and U6

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Cilantro 2

Plasmid

#74450

Purpose

Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-Cas9-2A-GFP-sgRNA

Plasmid

#124889

Purpose

Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

Depositor

Harvey Lodish

Article

Li et al Haematologica. 2016 Jun;101(6):e216-9. d

Insert

Cas9

Use

Retroviral

Tags

2A-GFP

Promoter

MSCV

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PARP1-E988K

Plasmid

#211580

Purpose

CMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Contains E988K mutation

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET-6xTR-TUBE

Plasmid

#110313

Purpose

E. coli expression and purification of 6xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

6xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon-PuroR

Plasmid

#174090

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

mNeonGreen

Use

Gene tagging

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pPolI_SARS-CoV-2_NLuc

Plasmid

#183953

Purpose

Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

Depositor

AJ te Velthuis

Article

Vial et al bioRxiv 2021.06.28.450211

Insert

SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)

Expression

Mammalian

Promoter

Mammalian Pol I

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5 CASC3 siRes1+2 f.l. WT

Plasmid

#158540

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant full-length CASC3

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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SpRY-ABE8e(V106W)

Plasmid

#198553

Purpose

Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpRY-ABE8e(V106W)

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-Alpha-Actinin-19

Plasmid

#54975

Purpose

Localization: Alpha Actinin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

actinin alpha 1 (ACTN1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC588 - pAAV c-fos Nuc-iRFP

Plasmid

#59132

Purpose

An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Nuclear-localized Infrared Fluorescent Protein

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

mFos

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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LRRK2-WT

Plasmid

#17609

Depositor

Ted Dawson

Article

West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

Insert

LRRK2 (LRRK2 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pUC57-rm_gCarvi-T2A

Plasmid

#190334

Purpose

Ratiometric reporter for cAMP

Depositor

Naoto Saitoh

Article

Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Insert

ratiometric gCarvi

Use

Unspecified

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pYY62-P1int-TP901-GFPdropout

Plasmid

#235773

Purpose

Plasmid used for constructing donor DNA for TP901-compatible p1 integration. This plasmid is a sfGFP dropout plasmid where sfGFP can be replaced by GOIs using Golden Gate Assembly.

Depositor

Chang Liu

Article

Pisera et al ACS Synth Biol. 2025 Apr 18;14(4):1002-1

Type

Empty backbone

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-p300

Plasmid

#191764

Purpose

Expression of EGFP fused to core p300 histone acetyltransferase

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

FRB-EGFP-p300 core (EP300 Human)

Tags

EGFP (N-terminal of p300 core) and FRB (N-termina…

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6e

Plasmid

#207855

Purpose

Mammalian expression of PE6e prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6e

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, K918A, K775R)

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_Divergent Donor

Plasmid

#229786

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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TIGRE-TRE-[poor kozak]Bxb1-ecDHFR-BGHpA

Plasmid

#158390

Purpose

Inducible Bxb1 integrase for mammalian cells

Depositor

Michael Elowitz

Article

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

Bxb1-ecDHFR

Expression

Mammalian

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT FUCCI

Plasmid

#136935

Purpose

donor plasmid for cardiac-specific FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Troponin T promoter

Use

Synthetic Biology

Expression

Mammalian

Promoter

cTnT

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-hSyn-mCherry

Plasmid

#87916

Purpose

sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

Depositor

Alex Hewitt

Article

Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

Insert

mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

hSyn

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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plenti-X1-Neo-RPN1-HA

Plasmid

#139868

Purpose

Lentiviral expression of HA-tagged (C terminal) human RPN1

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

RPN1 (Ribophorin1) (RPN1 Human)

Use

Lentiviral

Tags

HA

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-N1

Plasmid

#60561

Purpose

Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pMK262 (RAD21-mAC Neo)

Plasmid

#140538

Purpose

RAD21 tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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TRE-mCherry

Plasmid

#92202

Purpose

Fluorescent protein reporter construct

Depositor

Alice Ting

Article

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

Plasmid

#135454

Purpose

Bacterial expression of TEVΔ protease

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation improves stability. Truncated form…

Promoter

tac promoter

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSH424-ssr

Plasmid

#198026

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Sm resistance cassette

Expression

Bacterial

Promoter

SmR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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