162,326 results

1. pLminP_Luc2P_RE42

   Plasmid

   #90380

   Purpose

   GATA3 - Gata3 gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PPARg

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.23-5xE-box

   Plasmid

   #206841

   Purpose

   The plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).

   Depositor

   Tassula Proikas-Cezanne

   Article

   Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

   Insert

   5x E-box ((GCCACGTGCA)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-HA-human OCRL

   Plasmid

   #22207

   Depositor

   Pietro De Camilli

   Article

   Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

   Insert

   Oculocerebrorenal syndrome of Lowe (OCRL Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-p53/pRK5

   Plasmid

   #39237

   Depositor

   Xiaolu Yang

   Article

   Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETduet+p300HAT

   Plasmid

   #157793

   Purpose

   Expression of p300 HAT domain

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   p300HAT (EP300 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET FLIPPi-200u

   Plasmid

   #13555

   Depositor

   Wolf Frommer

   Article

   Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

   Insert

   FLIPPi G162A
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   G162A in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEGFP-HRas

   Plasmid

   #18662

   Depositor

   Karel Svoboda

   Article

   Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

   Insert

   H-Ras (HRAS Human)
   

   Use

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK792

   Plasmid

   #8830

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   TEV protease, S219P mutant
   

   Use

   Tags

   His, MBP (cleaved), and polyarginine

   Expression

   Bacterial

   Mutation

   S219P mutation

   Promoter

   Available since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXG260

   Plasmid

   #141282

   Purpose

   CMV:EGFP (as control for pXG237)

   Depositor

   Martin Kampmann

   Article

   Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Sept. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-sgRNA expression vector

    Plasmid

    #51132

    Purpose

    For in vitro transcription of sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3

    Plasmid

    #170163

    Purpose

    Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2s-P2A-H2B-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pS548∙CsR

    Plasmid

    #197859

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Tc resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PEM7

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_MMP-9-HA

    Plasmid

    #121172

    Purpose

    Expression of mouse MMP9

    Depositor

    Shinwu Jeong, M. Elizabeth Fini

    Article

    Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

    Insert

    MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV immediate/early

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP(C1)-PP1alpha

    Plasmid

    #44224

    Depositor

    Angus Lamond, Laura Trinkle-Mulcahy

    Article

    Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

    Insert

    PP1A (PPP1CA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid

    #170378

    Purpose

    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. peYFP-C1-mTOR

    Plasmid

    #73384

    Purpose

    Expresses YFP tagged mTOR

    Depositor

    Jie Chen, Taekjip Ha

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pST_140_LVL2 cam

    Plasmid

    #179334

    Purpose

    NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEB-Tg

    Plasmid

    #28220

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    SV40 large T
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET24a-VHH-std

    Plasmid

    #109417

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-Neo-TRE-CMV-Cre-rtTA

    Plasmid

    #165457

    Purpose

    Tetracycline inducible CRE expression from AAVS1 locus

    Depositor

    Madeline Lancaster

    Article

    Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

    Insert

    CRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSV40-mCherry-P2A-Hygro

    Plasmid

    #106478

    Purpose

    Expression of mCherry and Hygromycin resistance gene from SV40 promoter

    Depositor

    Alex Hewitt

    Inserts

    mCherry
    Hygromycin B resistance
    

    Use

    Tags

    Expression

    Mammalian and Plant

    Mutation

    Promoter

    SV40 promoter

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. 6xHsa.NFKB:d2EGFP

    Plasmid

    #239990

    Purpose

    Destabilized green fluorescent protein (d2EGFP) under transcriptional control of NF-kB activity

    Depositor

    Raquel Espin-Palazon

    Insert

    Destabilized EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cfos minimal promoter

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. SNAP-Drp1

    Plasmid

    #141159

    Purpose

    Mammalian expression and labeling of Drp1

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    DRP1 (DNM1L Human)
    

    Use

    Tags

    SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pS448∙CsR

    Plasmid

    #122096

    Purpose

    CRISPR/Cas9 counterselection in Gram-negative bacteria

    Depositor

    Pablo Ivan Nikel

    Article

    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pem7 and Pm

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP-NR2A

    Plasmid

    #17924

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    NMDAR2A (Grin2a Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDonor_ShCAST_kanR

    Plasmid

    #127924

    Purpose

    Kanamycin resistance donor for ShCAST

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. P3-dCas9-Tet1-GFP-Puro

    Plasmid

    #190728

    Purpose

    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    3XFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    pCAG

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. EF1a mCherry

    Plasmid

    #129431

    Purpose

    expression of mCherry from EF1a promoter

    Depositor

    Oskar Laur

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hSyn-eLACCO1.1

    Plasmid

    #168788

    Purpose

    Neuronal expression of fluorescent sensor for extracellular lactate

    Depositor

    Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLACCO1.1
    

    Use

    AAV

    Tags

    ER export and GPI

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

    Plasmid

    #154824

    Purpose

    (eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    Peft-3::wrmScarlet::tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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