We narrowed to 30,707 results for: PLE

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ptcALPS gag-gfp Blasti

Plasmid

#115806

Purpose

Express gag-gfp fusion driven by TK promoter

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Promoter

TK

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pTH-PanCen TALE-mVenusN

Plasmid

#191395

Purpose

TALE fused to N-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

N-terminal part of split mVenus

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-His-VHL-Flag

Plasmid

#29497

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Von Hippel-Lindau (VHL Human)

Tags

Flag and His

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
1BR9-mCherry

Plasmid

#193864

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged mCherry.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

mCherry

Tags

6xHis, GFP11, and R9

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9-mCherrySTOP

Plasmid

#193866

Purpose

E. coli expression vector for N-ter GFP11 tagged mCherry.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

mCherry

Tags

6xHis and GFP11

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ1359 - Intron GG2 - smu-1

Plasmid

#159805

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - smu-1

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMOS030E: Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

Plasmid

#163071

Purpose

Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS019E: Entry vector for: ClopHensorN chloride sensor (cytosolic)

Plasmid

#163066

Purpose

Entry vector for: ClopHensorN chloride sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ClopHensorN

Use

Gateway entry vector

Tags

tdTomato

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tpr-Si

Plasmid

#60918

Purpose

Expresses Flag tagged nucleoporin Tpr resistant to siRNA GCACAACAGGATAAGGTTA

Depositor

Vinay Nandicoori

Article

Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

Insert

TPR (TPR Human)

Expression

Mammalian

Mutation

Silent point mutations in siRNA target region; E1…

Promoter

CMV

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pSLIK TT 3xFLAG Luciferase neo

Plasmid

#98392

Purpose

Lentiviral vector for inducible expression of Luciferase with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

Luciferase

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSetd2#1/Cre

Plasmid

#89649

Purpose

Expresses a Setd2-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Setd2 (Setd2 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCRII-HSV2.3 TCR beta chain

Plasmid

#74077

Purpose

gBT-I TCR beta chain cDNA in pCRII

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR beta cDNA clone HSV2.3 (Tcrb Mouse)

Expression

Bacterial

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBDR

Plasmid

#62663

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

tdTomato

Use

Cre/Lox

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182

Plasmid

#22406

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

gawky, variant A (gw Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pGas-miRFP670nano3

Plasmid

#184672

Purpose

G protein alpha-subunit internally tagged with miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

G protein alpha-s-miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRS315-PGK-SEIPIN1

Plasmid

#96986

Purpose

Express GFP-tagged Arabidopsis SEIPIN1 gene in yeast cells.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Expression

Yeast

Promoter

Phosphoglycerate Kinase (PGK) promoter

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pASH3 [pmyo-3::BeCNG1-YFP]

Plasmid

#168167

Purpose

Expression of BeCNG1-YFP in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

BeCNG1-YFP

Tags

YFP

Expression

Worm

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCSN074

Plasmid

#166731

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E22: attB4-polyhedrinp>-attB1r

Plasmid

#162922

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-5A, 6A mMCL1-OM

Plasmid

#45818

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

5A, 6A mMCL-1-OM

Expression

Mammalian

Mutation

Changed Arginine to Alanine at 5th and 6th amino …

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-Tag/WT SPAST without 5'UTR

Plasmid

#87732

Purpose

Expresses WT spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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P16ΔS:S10-(MCS)-3xHA

Plasmid

#108183

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

P16ΔS

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmStaufen_B

Plasmid

#145947

Purpose

Insect Expression of DmStaufen

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

DmStaufen (stau Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 Actb-mRuby3 KI

Plasmid

#183437

Purpose

FlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and mRuby3 donor

Use

CRISPR

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC5 Tubb3-GFP KI

Plasmid

#183440

Purpose

FlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac-His-Flag-Tipin

Plasmid

#22890

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Tags

Flag- and His

Expression

Insect

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
A3G-Cas9n-UGI

Plasmid

#119139

Purpose

Base editor made from APOBEC3G

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3G-Cas9 nickase-UGI

Use

CRISPR

Mutation

None

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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PX544 SpCas9(del1098-1368)

Plasmid

#58889

Purpose

Truncated form of SpCas9 without C' domain

Depositor

Feng Zhang

Article

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

del amino acids 1098-1368

Available Since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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CAV1-D67G-HA

Plasmid

#27706

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1, mutant D67G (CAV1 Canis lupus familiaris)

Tags

HA

Expression

Mammalian

Mutation

mutant D67G

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-HaloLICNot

Plasmid

#140185

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, Halo-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

Halo

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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