We narrowed to 162,999 results for: addgene

1. pCG150_double_phiC31_attB

   Plasmid

   #169693

   Purpose

   cargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)

   Depositor

   Shih-Peng Chan

   Article

   Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GRIK1_pCSdest

   Plasmid

   #53832

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   GRIK1 (GRIK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pNTI647 dCas9-Mxi1 TetR KanMX

   Plasmid

   #139474

   Purpose

   Integrates CRISPRi effector and tet-responsive regulator

   Depositor

   Nicholas Ingolia

   Article

   McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

   Inserts

   dCas9-Mxi1
   Tet Repressor
   

   Use

   CRISPR

   Tags

   Mxi1 and NLS

   Expression

   Yeast

   Mutation

   D10A, H840A

   Promoter

   pGPM1 and pTEF1

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAF112

   Plasmid

   #135920

   Purpose

   All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

   Depositor

   Andrew S. Flies

   Article

   Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

   Type

   Empty backbone

   Use

   Luciferase; Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCH4 (denAsCas12a-KRAB lenti)

   Plasmid

   #217331

   Purpose

   Lentiviral expression of denAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   denAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mutation

   D908A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

   Plasmid

   #126580

   Purpose

   A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   OsARF16-PB1 domain
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-PITCh-TUBA1B

   Plasmid

   #207763

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-GfaABC1D-GRAB_g5-HT3.0

   Plasmid

   #208727

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   GfaABC1D

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #170816

   Purpose

   Lentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Insert

   NLS-mScarlet-I-P2A-BlastR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO mouse shRNA 1 rictor

    Plasmid

    #21341

    Depositor

    David Sabatini

    Article

    Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

    Insert

    rictor shRNA 1 (Rictor Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEM-Cas9HF1

    Plasmid

    #89961

    Purpose

    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    Cas9HF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. plRL117

    Plasmid

    #163635

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol1-UAS:ChR2(H134R)-tdTomato

    Plasmid

    #124237

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    ChR2(H134R)-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiPGK Puro DEST ERKKTRClover

    Plasmid

    #90227

    Purpose

    Lentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    ERK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWZL hygro H-Ras V12

    Plasmid

    #18749

    Depositor

    Scott Lowe

    Article

    Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

    Insert

    H-Ras V12 (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    V12

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRMC2-msfGFP

    Plasmid

    #194913

    Purpose

    Tetracycline inducible expression of msfGFP in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno Sequence followed by monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p7SL-neo-TET

    Plasmid

    #51285

    Purpose

    expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    7SL RNA (RN7SL2 Human)
    

    Use

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Mutation

    Promoter

    7SL upstream pollII enhancer

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. px459-TDP-43 sgRNA1

    Plasmid

    #133762

    Purpose

    Expresses Cas9 and human TDP-43 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pl-Synapsin-YFP-NaVII-III

    Plasmid

    #91246

    Purpose

    Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)

    Depositor

    Matthew Grubb

    Article

    Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.

    Insert

    Nav1.2 (Scn2a Rat)
    

    Use

    Lentiviral

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    The insert codes only for the intracellular loop …

    Promoter

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. CCT8_pCSdest

    Plasmid

    #53795

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    CCT8 (CCT8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    Sept. 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits