We narrowed to 17,734 results for: STO

1. pCAG-2dV-Camui (wt)

   Plasmid

   #220366

   Purpose

   Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Synthetic, Rat)
   

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-iSAM

   Plasmid

   #211495

   Purpose

   AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

   Depositor

   Antonella Fidanza, Lesley Forrester

   Article

   Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

   Inserts

   UniSAM insert
   TET-ON
   Neo Resistance
   Insulator
   

   Use

   CRISPR and Synthetic Biology

   Tags

   T2A-mCherry Tag

   Expression

   Mammalian

   Promoter

   EF1a, NA, Splicing acceptor, and TREGV promoter

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-SpCas9 blast

   Plasmid

   #104997

   Purpose

   This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-SpCas9 puro

   Plasmid

   #104994

   Purpose

   This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

   Plasmid

   #216250

   Purpose

   HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   HDR template for human SEC61B (SEC61B HDR template, Human, Synthetic)
   

   Use

   CRISPR; Hdr template

   Tags

   BSD.P2A.miniIAA7.3xFlag

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pF CAG luc IRES neo

   Plasmid

   #98294

   Purpose

   For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Inserts

   Firefly luciferase
   IRES
   Neomycin resistance gene (NeoR)
   

   Use

   Lentiviral and Luciferase

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. LifeAct-EGFP-APEX2 in pCSDEST2

   Plasmid

   #170523

   Purpose

   Vector for expression of LifeAct APEX2 in mammalian cells. Parton lab clone FTE

   Depositor

   Rob Parton

   Article

   Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

   Insert

   Cavin4 (Cavin4 Mouse)
   

   Tags

   APEX2-P2A-mKate2

   Expression

   Mammalian

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-human Erbb2-IRES-GFP

   Plasmid

   #91888

   Purpose

   Retroviral expression of human Erbb2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Erbb2 (ERBB2 Human)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Promoter

   MMLV gag

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-idCas9-KRAB-Hygro donor

   Plasmid

   #199621

   Purpose

   Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

   Insert

   AAVS1-idCas9-KRAB-Hygro
   

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. A24M04 mIgM heavy chain-pFEEKW

    Plasmid

    #234809

    Purpose

    Express membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1

    Depositor

    Susan Pierce

    Article

    Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

    Insert

    A24M04 membrane IgM heavy chain (IGHM Human)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Promoter

    EEK promoter

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-hygro-STING HAQ+V155M

    Plasmid

    #102601

    Purpose

    Express STING V55M HAQ for IFN-beta Luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    V155M R71H-G230A-R293Q

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD

    Plasmid

    #225699

    Purpose

    Lentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (K36M) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Mutation

    K36M

    Promoter

    pEF1A

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. A24M04 Ig kappa light chain-pFEEKW

    Plasmid

    #234810

    Purpose

    Express Ig kappa light chain containing the VL region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1

    Depositor

    Susan Pierce

    Article

    Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

    Insert

    A24M04 Igkappa light chain (IGKC, IGK Human)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Promoter

    EEK promoter

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. rag2:myr-Akt2_pISceI

    Plasmid

    #231495

    Purpose

    Expresses myristolated Akt2 in zebrafish lymphoid and mesenchymal cells

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    Akt2 (Akt2 Mouse)
    

    Use

    Zebrafish expression

    Tags

    N-terminal myristolation signal

    Available Since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFPC1-hVAP-A KD/MD

    Plasmid

    #104449

    Purpose

    Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed K94 to D and M96 to D

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. G9A[SET]-dCas9

    Plasmid

    #100089

    Purpose

    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    G9A (EHMT2 Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-G9A[SET]-dCas9-NLS

    Expression

    Mammalian

    Mutation

    G9A[SET] includes aa 829-1209

    Promoter

    CMV

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-10XHis-GST-HRV-dL5

    Plasmid

    #73214

    Purpose

    Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

    Insert

    EGFR (EGFR Human, Schistosoma japonicum)
    

    Tags

    10XHis-GST-HRV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+) K-Ras(WT)

    Plasmid

    #224283

    Purpose

    Cell transfection and expression of K-Ras

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    K-Ras (KRAS Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET30-2-PSAT1

    Plasmid

    #83908

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    phosphoserine aminotransferase 1 (PSAT1 Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA 3.1 KBTBD4 WT

    Plasmid

    #184627

    Purpose

    Mammalian expression vector with N-terminal 3xFlag for KBTBD4 WT expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 WT (KBTBD4 Human)
    

    Expression

    Mammalian

    Mutation

    no mutation

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid

    #223137

    Purpose

    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

    Plasmid

    #228252

    Purpose

    pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    SpCas9 P23H sgRNA, mTagBFP2
    

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and U6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. ITGB1

    Plasmid

    #51920

    Purpose

    Expresses full-length Integrin beta-1 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ITGB1 (ITGB1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Stop Codon at amino acid 728, before CD4, bio and…

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBFC0619

    Plasmid

    #186690

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    

    Expression

    Bacterial

    Promoter

    PLac

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET-26b-Nb127D01-ALFA-His

    Plasmid

    #171568

    Purpose

    Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-ALFA-His (CXCR2 Human)
    

    Tags

    ALFA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. LMPd Amt OXCT1

    Plasmid

    #209407

    Purpose

    Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

    Depositor

    Russell Jones

    Article

    Luda et al Immunity. 2023 Sep 12;56(9):2021-2035.e8

    Insert

    Oxct1 shRNA (Oxct1 Mouse)
    

    Use

    Retroviral

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD

    Plasmid

    #216248

    Purpose

    HDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSD

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    HDR template for human RANGAP1 (RANGAP1 Human)
    

    Use

    CRISPR and TALEN; Hdr template

    Tags

    miniIAA7.3xFlag.P2A.BSD

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pX458_mCherry-EWSR1-gRNA

    Plasmid

    #237683

    Purpose

    Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to EWSR1 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EWSR1 gRNA (EWSR1 Human)
    

    Use

    CRISPR

    Tags

    mCherry

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pBiFC-PBZ-4A-VC

    Plasmid

    #110649

    Purpose

    expresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

    Insert

    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    

    Tags

    HA and VC

    Expression

    Mammalian

    Mutation

    C379A, C385A, C421A and C427A

    Promoter

    CMV

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFPC1-hVAP-B KD/MD

    Plasmid

    #104450

    Purpose

    Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein B (VAPB Human)
    

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    changed K87 to D and M89 to D

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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