174,131 results

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pMXs-ms-Klf2

Plasmid

#50786

Purpose

retroviral expression of mouse Klf2

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Klf2 (Klf2 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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flag-hKLF6 (1006)

Plasmid

#49488

Purpose

expresses human flag tagged KLF6 in mammalian cells

Depositor

Scott Friedman

Article

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

KFL6 (KLF6 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli3/Flag

Plasmid

#51246

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only
pLenti-ReaChR-citrine

Plasmid

#50956

Purpose

ReaChR-citrine in lentival transfer vector under human synapsin promoter

Depositor

Roger Tsien

Article

Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

Insert

ReaChR-citrine

Use

Lentiviral

Tags

citrine

Promoter

human synapsin promoter

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCDEF3‐hTIM3 (no tag)

Plasmid

#49212

Purpose

expresses human Tim3

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (HAVCR2 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-HA-CAD

Plasmid

#46238

Purpose

For expression of HA and FLAG-tagged murine CAD in mammalian cells

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pGL410_Ins2500

Plasmid

#49058

Purpose

Expresses luciferase under control of mouse insulin 2 gene promoter

Depositor

Kevin Ferreri

Article

Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

Insert

mouse Ins2 promoter (Ins2 Mouse)

Expression

Mammalian

Promoter

Ins2 promoter

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-ATXN1

Plasmid

#48189

Purpose

Expresses Ataxin1

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Ataxin1 (ATXN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA

Plasmid

#47458

Purpose

LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

TALE (Neurog2)-GS-CIB1(NLS*,∆318-334)

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-hSC

Plasmid

#46896

Purpose

expression of scrambled shRNA

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

scrambled

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pWJ68

Plasmid

#46571

Purpose

Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
w-dcas9
CRISPR array

Use

CRISPR

Tags

rpoZ

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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BECLIN-HA WT

Plasmid

#46993

Purpose

Expresses Beclin1 in mammalian cells

Depositor

Kunliang Guan

Article

Russell et al Nat Cell Biol. 2013 Jul;15(7):741-50. do

Insert

becn1 (Becn1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only
pRSV-PKI-v2

Plasmid

#45066

Purpose

Expression of PKI (PKA inhibitor)

Depositor

Richard Maurer

Insert

cAMP-dependent protein kinase inhibitor alpha

Expression

Mammalian

Promoter

RSV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ909

Plasmid

#44480

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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HA Depdc5 pRK5

Plasmid

#46327

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Tags

HA

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
EF13E

Plasmid

#42942

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

EN- Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

AgeI-BclI fragment swapped with nt 1927-3708 from…

Promoter

CMV MIE, L1 5'UTR

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only
EF12J

Plasmid

#42943

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

EN+ Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

AgeI-BclI fragment swapped with nt 1927-3708 from…

Promoter

CMV MIE, L1 5'UTR

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-HBXIP

Plasmid

#42326

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

HBXIP (LAMTOR5 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pVLAD6

Plasmid

#41850

Depositor

Chris Garcia

Article

Dukkipati et al Protein Expr Purif. 2008 Dec;62(2):160-7

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 ICv-6MT

Plasmid

#41730

Depositor

Raphael Kopan

Article

Schroeter et al Nature. 1998 May 28;393(6683):382-6.

Insert

Notch-1 Intracellular Domain (Notch1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 protein beginning at Vali…

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pHMGWA-Pa14Csy4H29A

Plasmid

#41092

Depositor

Jennifer Doudna

Article

Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

Insert

Csy4

Tags

His6-MBP

Mutation

His29Ala mmutation to abolish ribonuclease activi…

Promoter

T7

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ERBB2 A775_G776insYVMA

Plasmid

#40982

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

A775_G776insYVMA

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pMIEG3-JunB

Plasmid

#40349

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

JunB (Jun Mouse)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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AMPK-β1-FLAG

Plasmid

#40602

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-β1 (Prkab1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pFA6a-13Myc-kanMX6

Plasmid

#39294

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

13Myc, A. gossypii translation elongation factor …

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-LC3

Plasmid

#38195

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA

Plasmid

#37090

Purpose

Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

hChr2(H134R)-mCherry

Use

AAV and Cre/Lox; Cre-off

Tags

mCherry

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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FUGW-H1-GFP-neomycin

Plasmid

#37632

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Neo-Bam E2F3b

Plasmid

#37975

Depositor

Jacqueline Lees

Insert

E2F3b (E2F3 Human)

Expression

Mammalian

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-Venus-pLenti6

Plasmid

#36948

Depositor

William Kaelin

Article

Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

Insert

Venus (EYFP)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

-F46L/F64L/V68L/M153T/V163A/S175G

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge