We narrowed to 15,833 results for: grna

1. pCX459

   Plasmid

   #229214

   Purpose

   CRISPR array plasmid for MAP1B (Spacers 1-24)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for MAP1B (Spacers 1-24)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCX460

   Plasmid

   #229215

   Purpose

   CRISPR array plasmid for MAP1B (Spacers 25-48)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for MAP1B (Spacers 25-48)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Rictor_1 shRNA

   Plasmid

   #1853

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   Rictor (RICTOR Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 10, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS300

   Plasmid

   #22846

   Purpose

   Backbone for expressing plant artificial miRNAs

   Depositor

   Detlef Weigel

   Article

   Schwab et al Plant Cell. 2006 May . 18(5):1121-33.

   Insert

   MIR319a (MIR319a Mustard Weed)
   

   Use

   RNAi; Plant gene inactivation

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Jan. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVA451-Cpf1

   Plasmid

   #192362

   Purpose

   CRISPR-based vector for genome editing

   Depositor

   Juana María Navarro Llorens

   Article

   Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

   Insert

   Cas12a, gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. multi-shRNA vector

   Plasmid

   #12391

   Depositor

   Zuoshang Xu

   Article

   Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

   Insert

   mSOD2 shRNA (Sod2 Mouse)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAS9-mCherry empty

   Plasmid

   #80975

   Purpose

   Cas9, mCherry, and sgRNA expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rictor_2 shRNA

   Plasmid

   #1854

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   Rictor (RICTOR Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUCSh5vio-2300

   Plasmid

   #170987

   Purpose

   Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   Sh-CAST sgRNA 2300 violacein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   placI

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEVA351-Cpf1

    Plasmid

    #192361

    Purpose

    CRISPR-based vector for genome editing

    Depositor

    Juana María Navarro Llorens

    Article

    Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

    Insert

    Cas12a, gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiGuide-tagBFP

    Plasmid

    #217007

    Purpose

    lentiviral plasmid for gRNA cloning with selectable tagBFP expression

    Depositor

    Deepta Bhattacharya

    Article

    D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

    Plasmid

    #137993

    Purpose

    Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

    Depositor

    Amy Brock

    Article

    Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHelper_ShCAST

    Plasmid

    #127922

    Purpose

    Expresses ShCAST and a crRNA targeting PSP1

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330_SYCP3_nterm

    Plasmid

    #222524

    Purpose

    Cas9/sgRNA plasmid for targeting SYCP3

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    Cas9, SYCP3 sgRNA (SYCP3 Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. p426*-SpSgH

    Plasmid

    #136993

    Purpose

    Helper plasmid for SpCas9 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-As-crRNA

    Plasmid

    #78956

    Purpose

    Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJW1311

    Plasmid

    #61254

    Purpose

    Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E) targeting Y61A9L9.1
    

    Use

    CRISPR; Cloning template

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PX851

    Plasmid

    #62883

    Purpose

    N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PX852

    Plasmid

    #62884

    Purpose

    C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS SV40, and NLS nucleoplasmin

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-Universal

    Plasmid

    #52694

    Purpose

    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

    Depositor

    Sebastian Lourido

    Inserts

    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    

    Use

    CRISPR; Toxoplasma gondii

    Tags

    3X FLAG and Nuclear localization signal

    Expression

    Mutation

    Promoter

    TgTUB1 and Toxoplasma U6

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. YN2_1_LT58_X2site

    Plasmid

    #177705

    Purpose

    Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p426*-SaSgH

    Plasmid

    #136994

    Purpose

    Helper plasmid for SaCas9 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TtRMPVIR

    Plasmid

    #27995

    Depositor

    Scott Lowe

    Article

    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

    Insert

    TtRMPVIR (shRen)
    

    Use

    RNAi and Retroviral; Fluorescent reporters

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pENTR U6-hTERTsh

    Plasmid

    #111385

    Purpose

    Knock down of hTERT transcript

    Depositor

    Klaus Holzmann

    Article

    Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

    Insert

    shRNA C3 against hTERT
    

    Use

    Gateway entry cloning

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. U6>Control(F+E)

    Plasmid

    #60006

    Purpose

    U6 promoter driving control (F+E) sgRNA

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    Control (F+E) sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ciinte.U6

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJMP2832

    Plasmid

    #160672

    Purpose

    Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Insert

    gmc6 (gfp) sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJMP2820

    Plasmid

    #160669

    Purpose

    Mobile CRISPRi sfGFP 'test', empty sgRNA

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Insert

    empty sgRNA (BsaI)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PX854

    Plasmid

    #62886

    Purpose

    C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa573-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS SV40, and NLS nucleoplasmin

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBPGUw-HACK-G4>QF2

    Plasmid

    #80277

    Purpose

    GAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    QF2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCRISPR::rpsL

    Plasmid

    #44505

    Purpose

    A crRNA expression plasmid specific to the rpsL allele.

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    CRISPR::rpsL (rpsL )
    

    Use

    CRISPR; E.coli

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge