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Showing: 1601 - 1650 of 6475 results

  7. pAAV-synp-F-synaptophysin-GCaMP6f

    Plasmid
    #102996
    Purpose
    Expresses synaptophysin fused GCaMP6f under the synapsin promoter
    Depositor
    David Cox
    Insert
    Synaptophysin-GCaMP6f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    102996-plasmid-map-sequence-id-218069

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  8. pAAV-tMCK-mem-TurboID

    Plasmid
    #149410
    Purpose
    Muscle-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection
    Depositor
    Jonathan Long
    Insert
    mem-TurboID
    Use
    AAV
    Tags
    HA and V5
    Expression
    Mutation
    Promoter
    tMCK
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149410-plasmid-map-sequence-id-299812

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  12. pAAV-EF1α1.1-ChrimsonR-tdTomato

    Plasmid
    #122064
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1a（1.1kb short version)
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122064-plasmid-map-sequence-id-242270

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  15. pAAVS1-Puro-CAG-mNG2(1-10)

    Plasmid
    #206042
    Purpose
    Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.
    Depositor
    Alisa Piekny
    Article
    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7
    Insert
    AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
    Use
    CRISPR and Synthetic Biology; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    206042-plasmid-map-sequence-id-411117

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  19. pAAV-CaMKIIa-ChRger2-TS-EYFP

    Plasmid
    #127238
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger2-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIa
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127238-plasmid-map-sequence-id-247000

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  20. pAAV-hSyn-ChRger1-TS-EYFP

    Plasmid
    #127243
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger1-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127243-plasmid-map-sequence-id-247230

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  26. qTAG-AAVS1-Ef1a-Puro

    Plasmid
    #227266
    Purpose
    Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227266-plasmid-map-sequence-id-452014

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  36. AAV-CAG-jGCaMP8m-WPRE

    Plasmid
    #179255
    Purpose
    AAV-mediated expression of GCaMP8m under the CAG promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8m
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    179255-plasmid-map-sequence-id-354032

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  40. pAAV-hSyn-EGFP-KASH

    Plasmid
    #209201
    Purpose
    Neuronal expression of EGFP tagged KASH protein domain
    Depositor
    Larry Zweifel
    Insert
    eGFP-KASH
    Use
    AAV
    Tags
    KASH localization domain
    Expression
    Mammalian
    Mutation
    Promoter
    hsyn
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209201-plasmid-map-sequence-id-418201

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  50. AAV-hSyn-RiboL1-jGCaMP8m

    Plasmid
    #169248
    Purpose
    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Insert
    RiboL1-jGCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    169248-plasmid-map-sequence-id-349877

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Showing: 1601 - 1650 of 6475 results