We narrowed to 7,666 results for: trac

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pPBpuro-4493NES(Booster-PKA)

Plasmid

#184709

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-3nmt1

Plasmid

#105162

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

hphMX6-3nmt1

Tags

3nmt1

Expression

Bacterial and Yeast

Promoter

3nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-HBTH

Plasmid

#127883

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-HBTH

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-A4DARPin

Plasmid

#198288

Purpose

Produces PVCs retargeted with anti-lysozyme DARPin A4

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-A4DARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-NKB

Plasmid

#184609

Purpose

For expression of a fluorescent sensor for neurokinin B in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NKB

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-GCaMP6s

Plasmid

#184712

Purpose

Calcium biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

GCaMP6s

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a - NR

Plasmid

#89625

Purpose

Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Nitrate reductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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peCALWY-NB

Plasmid

#22239

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY non-binding variant

Expression

Mammalian

Mutation

C284S, C287S, C413S and C416S mutations linker c…

Available Since

Jan. 11, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-PICK

Plasmid

#31613

Depositor

Robert Malenka

Article

Citri et al J Neurosci. 2010 Dec 8. 30(49):16437-52.

Insert

PICK1 (shRNA insensitive) +shRNA towards PICK1 (Pick1 Rat)

Use

Lentiviral

Tags

GFP, HA, and PICK1 shRNA

Expression

Mammalian

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pJBL7004

Plasmid

#136943

Purpose

Malachite green aptamer, T7 promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

malachite green aptamer (MGA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pT7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBT264

Plasmid

#27438

Depositor

Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

TRE-tdTomato-myc flanked by insulators

Use

Mouse Targeting; Tta dependent

Tags

Myc

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCL66-decoder

Plasmid

#159453

Purpose

Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pfus1mut-tetR-nls-malE-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfus1mut

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Th

Plasmid

#105988

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

tyrosine hydroxylase (Th Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnfΔpvc15_pvc12-HiBiT

Plasmid

#198309

Purpose

Negative control for payload loading assay; produces PVCs deficient for payload loader (pvc15)

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnfΔpvc15_pvc12-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y925F

Plasmid

#50509

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y925F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

Plasmid

#13005

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 1 delta CC (SULF1 Human)

Expression

Mammalian

Mutation

changed Cysteine 87 to Alanine

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-EPN51 (R40A)

Plasmid

#80109

Purpose

Late1-O3-33-myc-PH(R40A)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-51 (R40A)

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pTn7-SCOUT21.RY

Plasmid

#201006

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-mCherry-sYFP2, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

mcherry
sYFP2

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT21.YB

Plasmid

#201008

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-sYFP2-TagBFP, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sYFP2
TagBFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5DL491-492

Plasmid

#198290

Purpose

Produces PVCs retargeted with non-binding mutant of Ad5 knob

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5DL491-492

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-MCH

Plasmid

#184606

Purpose

For expression of a fluorescent sensor for melanin-concentrating hormone in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-MCH

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*

Plasmid

#80039

Purpose

Expression of EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-HF

Plasmid

#127882

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3F tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJPT003-PM-HaloTag-1x FLAG

Plasmid

#202494

Purpose

Express "PM" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Tags

1x FLAG and Palmitoylation-Myristoylation Site

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-H3FAID

Plasmid

#127884

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3FAID tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F-AID

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pUHD-AID

Plasmid

#121066

Purpose

vector expresses AID-fusion protein under TRE promoter and with puromycin resistance selection

Depositor

Randy Poon

Article

Ng et al Cell Cycle. 2018 Dec 23. doi: 10.1080/15

Type

Empty backbone

Tags

AID

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pKH3-avian RSK1 K112/464R

Plasmid

#8981

Depositor

John Blenis

Article

Roux et al Mol Cell Biol. 2003 Jul . 23(14):4796-80

Insert

RSK (RPS6KA1 Chicken)

Tags

3x HA

Expression

Mammalian

Mutation

K112R, K464R

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-PTB

Plasmid

#21929

Depositor

Phil Sharp

Article

Garcia-Blanco et al Genes Dev. 1989 Dec . 3(12A):1874-86.

Insert

PTB (PTBP1 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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mCherry_LD 0_TEV

Plasmid

#58868

Purpose

MESA protease chain with mCherry ectodomain and no linker domain

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with mCherry ectodomain and TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pRSET-His-APEX2

Plasmid

#201471

Purpose

Recombinant soluble APEX2 for SLAPSHOT labeling

Depositor

Yuh-Nung Jan

Article

Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

Insert

APEX2

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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TwinStrep-SUMO-Pdp1

Plasmid

#198320

Purpose

For affinity purification of Pdp1 payload

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

TwinStrep-SUMO-Pdp1

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBH546_Tet-Cas14b1Locus

Plasmid

#112503

Purpose

Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b1

Use

CRISPR

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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mUAVbla:tetR 5′

Plasmid

#149379

Purpose

mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

Depositor

Mark Pallen

Article

Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

Insert

tetA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBH547_Tet-Cas14b2Locus

Plasmid

#112504

Purpose

Expresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b2

Use

CRISPR

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBT140

Plasmid

#27493

Depositor

Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

Nuclear localization signal-Cre

Tags

nuclear localization signal

Expression

Mammalian

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBJ1-rat Beta2m

Plasmid

#121670

Purpose

Expresses rat β-2-microglobulin in mammalian cells

Depositor

Pamela Bjorkman

Article

Tesar et al Traffic. 2006 Sep;7(9):1127-42.

Insert

rat beta-2- microglobulin (B2m Rat)

Expression

Mammalian

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-5HT

Plasmid

#184597

Purpose

For expression of a fluorescent sensor for serotonin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-5HT

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-HaloGFP-Na2.4

Plasmid

#228470

Purpose

Bacterial expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

Depositor

Robert Campbell

Article

Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

Insert

HaloGFP-Na2.4

Expression

Bacterial

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pREVTRE-AID

Plasmid

#121067

Purpose

Retrovirus vector expresses AID-fusion protein under TRE promoter and with hygromycin resistance selectio

Depositor

Randy Poon

Article

Ng et al Cell Cycle. 2018 Dec 23. doi: 10.1080/15

Type

Empty backbone

Use

Retroviral

Tags

AID

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPy-CAG-GFP-IRES-Pac

Plasmid

#48759

Purpose

CAG promoter driving GFP-IRES-Puromycin Acetyl Transferase

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

EGFP

Use

Ppy

Expression

Mammalian

Promoter

CAG

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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DL147_plenti_TRE_HT-iPAK4

Plasmid

#187447

Purpose

Tet-On inducible expression of HaloTag-iPAK4 in a lentiviral backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

INKA-box Pak4

Use

Lentiviral

Tags

halo tag

Expression

Mammalian

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-SST

Plasmid

#184619

Purpose

For expression of a fluorescent sensor for somatostatin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-SST

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CD200Cd4d3+4-bio

Plasmid

#32404

Purpose

A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200

Tags

Cd4d3+4 and biotinylation peptide

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Gag-FKBP-Myc

Plasmid

#177909

Purpose

HIV Gag-FKBP-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

HIV Gag (gag )

Tags

FKBP12 and Myc tag

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CD200Cd4d3+4-blac

Plasmid

#32405

Purpose

A positive control receptor bait (rat CD4d3+4-blac) and prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200

Tags

Cd4d3+4 and beta-lactamase

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
py100-sgRNA_hVEGFA

Plasmid

#198282

Purpose

Expression of SpCas9 guide RNA specific for human VEGFA locus

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

py100-sgRNA_hVEGFA

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSIIpuro-4493NES(Booster-PKA)

Plasmid

#184710

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TwinStrep-SUMO-Pnf

Plasmid

#198321

Purpose

For affinity purification of Pnf payload

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

TwinStrep-SUMO-Pnf

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pALS3-Ma-SUMO-sfGFP-WT

Plasmid

#212122

Purpose

Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

Depositor

Ryan Mehl

Inserts

SUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)

Tags

His6 and SUMO

Expression

Bacterial

Promoter

araC and lpp

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWP78-PVCpnf_pvc13-MoonTag

Plasmid

#198299

Purpose

Produces PVCs retargeted with MoonTag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-MoonTag

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 7,666 results for: trac