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pQdC12a

Plasmid

#191356

Purpose

Clostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expression

Depositor

Andrew Tolonen

Publication

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Inserts

PGusA2-TetO2/1-dCas12a (LbCpf1)
miniPthl-tetR

Expression

Bacterial

Promoter

PGus2A-TetO2/1 and miniPthl

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMK433 (mAID-P2A-BSD)

Plasmid

#168228

Purpose

C-terminal tagging with mAID

Depositor

Masato Kanemaki

Publication

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

mAID-P2A-BSD

Use

CRISPR

Expression

Mammalian

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCherry-NES

Plasmid

#186349

Purpose

Cytosolic mCherry expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Publication

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCherry-NES

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVITRO1-mCardinal-CAAX

Plasmid

#184451

Purpose

Plasma membrane-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Publication

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-CAAX

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDGE651

Plasmid

#153229

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCS2+FucciOrange

Plasmid

#128427

Purpose

Contains insert allowing in-vivo visualization of Cdk1 protein (G1)

Depositor

Rusty Lansford

Publication

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

FucciOrange

Expression

Mammalian

Promoter

CMV & SP6

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pT2ADWpuro_2paRAF

Plasmid

#129653

Purpose

For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

Depositor

Michiyuki Matsuda

Publication

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX

Expression

Mammalian

Promoter

CAG

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pC010 LshCas13a locus with nontargeting spacer

Plasmid

#91901

Purpose

LshCas13a locus into pACYC184 with nontargeting spacer

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LshCas13a locus with non-targeting spacer

Expression

Bacterial

Available since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pMD19T-slr0230-PL22-sgRNANT1-KmR

Plasmid

#73224

Purpose

Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

Depositor

Paul Hudson

Publication

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

PL22-sgRNA NT1

Expression

Bacterial

Promoter

PL22

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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bactin-loxp-BFP-loxp-dsred-pBSII

Plasmid

#68972

Purpose

for transgenic animals

Depositor

David Traver

Publication

Kobayashi et al Nature. 2014 Aug 21;512(7514):319-23. do

Inserts

lox-BFP-loxp
dsred

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pNAS1B split mCherry N-link/link-C

Plasmid

#61965

Purpose

Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)

Depositor

Lynne Regan

Publication

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

N-mCherry-link
link-C-mCherry

Expression

Bacterial

Promoter

PBAD and PLtetO

Available since

Feb. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-link-yoTagRFP657-CaURA3

Plasmid

#44882

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFP657 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP657

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPSmOrange-Kan

Plasmid

#44951

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPSmOrange

Expression

Yeast

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pGV7 (CK2alpha' K69M, CK2beta)

Plasmid

#27095

Depositor

David Litchfield

Publication

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha' K69M (CSNK2A2 Human)

Use

Tetracycline-regulated expression

Tags

HA tag on CK2alpha' and Myc on CK2beta

Expression

Mammalian

Mutation

K69M on CK2alpha'

Promoter

bidirectional tet-responsive promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-ND1 Left -GSVG-UGI

Plasmid

#187412

Purpose

human mitochondrial DNA ND1 target TALE

Depositor

Jin-Soo Kim

Publication

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA GSVG variant

Expression

Mammalian

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Beta4Gal-HaloTag7

Plasmid

#175536

Purpose

Expression of HaloTag7 at the at the Golgi apparatus of mammalian cells

Depositor

Kai Johnsson

Publication

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Beta4Gal-HaloTag7

Expression

Mammalian

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE668

Plasmid

#153233

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE666

Plasmid

#153231

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_hU6

Plasmid

#102843

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Publication

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_hU6promoter

Use

As pcr template

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS-TRE-SNAPf-WPRE

Plasmid

#104106

Purpose

Expresses SNAPf under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Publication

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

SNAPf

Expression

Mammalian

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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