We narrowed to 10,749 results for: AGA

1. Cdk13_intron3_sg3_pX458

   Plasmid

   #127338

   Purpose

   sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #3

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Mouse Cdk13 Intron 3 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   U6 Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

   Plasmid

   #226991

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 2a to create OPA1-del_exon5
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

   Plasmid

   #226992

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 2b to create OPA1-del_exon5
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX459_TELO2_gRNA

   Plasmid

   #224379

   Purpose

   Targets TELO2 for dTAG N terminal knock-in cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   TELO2 (TELO2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKM-U6-reRNA-hsRUNX

   Plasmid

   #205639

   Purpose

   reRNA guide targeting Homo sapiens RUNX

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-hsRUNX (RUNX1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PD2529 human beta1 ecto

   Plasmid

   #221398

   Purpose

   Mammalian expression of human integrin beta1 ectodomain

   Depositor

   Timothy Springer

   Article

   Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

   Insert

   integrin beta1 ectodomain (ITGB1 Human)
   

   Use

   Tags

   AVI tag, HRV3C cleavage site, basic coil, HA tag,…

   Expression

   Mammalian

   Mutation

   codon optimized for human mature _1 residues Q1 t…

   Promoter

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Beta1 Q280* K418* full-length

   Plasmid

   #221406

   Purpose

   Mammalian expression of human integrin beta1 Q280* K418* full-length

   Depositor

   Timothy Springer

   Article

   Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

   Insert

   integrin beta1 Q280* K418* full-length (ITGB1 Human)
   

   Use

   Tags

   CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

   Expression

   Mammalian

   Mutation

   codon-optimized mature sequence

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Beta1 N391* N562* full-length

   Plasmid

   #221407

   Purpose

   Mammalian expression of human integrin beta1 N391* N562* full-length

   Depositor

   Timothy Springer

   Article

   Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

   Insert

   integrin beta1 N391* N562* full-length (ITGB1 Human)
   

   Use

   Tags

   CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

   Expression

   Mammalian

   Mutation

   codon-optimized mature sequence

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LT3GEPIR-Rb1shRNA1

   Plasmid

   #220584

   Purpose

   To inducibly knockdown Rb1 expression

   Depositor

   Sean Lee

   Article

   Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

   Insert

   Rb1 shRNA1 (RB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G (TetOP) promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLentiCriprV2-sgRNA-CRY2-#1

    Plasmid

    #189989

    Purpose

    Lentiviral Cas9/sgRNA vector targeting C-terminus of hCRY2, works with Addgene 189983-189986

    Depositor

    Achim Kramer

    Article

    Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Insert

    Cryptochrome-2 (CRY2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5'-3xFLAG Chtop+exon_siResist minimal CMV pCDNA5

    Plasmid

    #212083

    Purpose

    LR vector for integration of Chtop+exon(138nt)_siResist into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Chtop+exon(138nt)_siResist (Chtop Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCCTATATGTCCAAGACCA

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. 5'-3xFLAG Chtop-exon_siResist minimal CMV pCDNA5

    Plasmid

    #212082

    Purpose

    LR vector for integration of Chtop-exon_siResist into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Chtop-exon_siResist (Chtop Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCCTATATGTCCAAGACCA

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR223-CMV-Cre-U6-sgKdm6a#4-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

    Plasmid

    #209061

    Purpose

    Entry vector that endcodes sgRNAs against mouse Kdm6a, Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

    Depositor

    Matthew Oser

    Article

    Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

    Insert

    KDM6A
    

    Use

    Gateway vector to be used for lr reaction

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiCRISPR v2-sgABI2-1

    Plasmid

    #210139

    Purpose

    knock out ABI2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Abl interactor 2 (ABI2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lentiCRISPR v2-sgCYFIP1-1

    Plasmid

    #210137

    Purpose

    knock out CYFIP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiCRISPR v2-sgHSPC300-1

    Plasmid

    #210141

    Purpose

    knock out HSPC300 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Protein BRICK1 (BRK1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiCRISPR v2-sgCYFIP1-2

    Plasmid

    #210138

    Purpose

    knock out CYFIP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV_dCas9-KRAB_sgSTK3i_#2 (PuroR)

    Plasmid

    #209772

    Purpose

    CRISPRi for STK3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    dCas9-KRAB, sgSTK3i (STK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1 puro shCALM2-1

    Plasmid

    #193699

    Purpose

    Constitutive lentiviral expression of CALM2 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    CALM2 (CALM2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1 puro shCALM2-2

    Plasmid

    #193700

    Purpose

    Constitutive lentiviral expression of CALM2 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    CALM2 (CALM2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Lenti-sgMroh2b#2/Cre

    Plasmid

    #193220

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Mroh2b gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgMroh2b#2 (Heatr7b2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV.CBA.YFP.miR-E_shIrak1-3

    Plasmid

    #180399

    Purpose

    Producing AAV that encodes mouse Irak1 shRNA-3 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shIrak1-3 (Irak1 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

    Plasmid

    #183457

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

    Plasmid

    #183458

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. psgRNA-2xMS2-mSAT

    Plasmid

    #181908

    Purpose

    Single guide RNA with 2XMS2 loops targeting mouse major satellite repeats

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    sgRNA-2XMS2-mSAT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGMC00003

    Plasmid

    #166720

    Purpose

    sgRNA against mouse Kitl

    Depositor

    Raj Chari

    Article

    Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

    Insert

    Kitl (Kitl Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGMC00004

    Plasmid

    #166721

    Purpose

    sgRNA against mouse Kitl

    Depositor

    Raj Chari

    Article

    Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

    Insert

    Kitl (Kitl Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LentiGuide-Neo-NCOA7g3 (BB36)

    Plasmid

    #139454

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. px335-sgRNA-EF(Cas9n)-mHdac1-L2

    Plasmid

    #122307

    Purpose

    Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Hdac1 (Hdac1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPN431

    Plasmid

    #137869

    Purpose

    Expression of gRNA a2 targeting TCF4 for CRISPRa; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge