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Showing: 10081 - 10110 of 13600 results

  3. pAAV-CMV-dSa VP64 Actc1

    Plasmid
    #99691
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG) (Actc1 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Mutation
    dead Cas9
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    99691-plasmid-map-sequence-id-194530

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  4. pMTB2-Avi-Cerulean-Rpl10a

    Plasmid
    #79880
    Purpose
    Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    Tags
    Avi
    Expression
    Bacterial
    Promoter
    Beta-actin2
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79880-plasmid-map-sequence-id-149770

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  13. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80065
    Purpose
    Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Zic2a enhancer driving c-fos promoter
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80065-plasmid-map-sequence-id-152617

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  15. Ntng2.a-Fc-His

    Plasmid
    #72120
    Purpose
    Expresses the extracellular region of the Netrin G2, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng2.a (Ntng2 Mouse)
    Tags
    Fc-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72120-plasmid-map-sequence-id-131306

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  16. Ntng1.b-Fc-His

    Plasmid
    #72111
    Purpose
    Expresses the extracellular region of the Netrin G1, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Ntng1.b (Ntng1 Mouse)
    Tags
    Fc-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72111-plasmid-map-sequence-id-139922

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  24. BMR1_02g00320-bio-His

    Plasmid
    #108028
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_02g00320 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Insert
    BMR1_02g00320
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available Since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108028-plasmid-map-sequence-id-213147

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  25. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80061
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Myl7 promoter
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80061-plasmid-map-sequence-id-152595

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  28. pEnt R3L2 TetO(sh) mCh-Rs1-2

    Plasmid
    #24417
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Plasmid also known as pEntR3L2 TetO-mCh-Rs1.
    Depositor
    Edward Hsiao
    Article
    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
    Insert
    Rs1
    Use
    Entry vector
    Tags
    FLAG and mCherry-P2A
    Mutation
    D100A
    Promoter
    short TetO, miniCMV
    Available Since
    March 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    24417-plasmid-map-sequence-id-11414

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Showing: 10081 - 10110 of 13600 results