We narrowed to 2,810 results for: ALC

1. CD99-CD4d3+4-bio

   Plasmid

   #73097

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding CD99 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   CD99 (CD99 Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. PH-Btk(R28C)-GFP

   Plasmid

   #51464

   Purpose

   Non-PIP3 binding control for PH-Btk-GFP

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   BTK (aa 1–177) (BTK Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R28C

   Promoter

   CMV

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pSS1 (hLPL-FLAG)

   Plasmid

   #102447

   Purpose

   Expresses FLAG-tagged human LPL in mammalian cells

   Depositor

   Brandon Davies

   Article

   Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

   Insert

   Lipoprotein lipase (LPL Human)
   

   Use

   Tags

   6x His and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SBC015866

   Plasmid

   #226280

   Purpose

   Expresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.

   Depositor

   Rainer Breitling

   Article

   Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.

   Inserts

   4'-phosphopantetheinyl transferase Sfp
   Cinnamyl alcohol dehydrogenase
   Carboxylic acid reductase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. CR1-isoformB-CD4d3+4-bio

   Plasmid

   #73099

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding CR1-isoformB with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   CR1 isoform B (CR1 Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPBC-LG3-tdT

   Plasmid

   #62810

   Purpose

   pPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.

   Depositor

   Karen Wilcox

   Article

   Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

   Insert

   ITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CD47-CD4d3+4-bio

   Plasmid

   #73121

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding CD47 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   CD47 antigen (CD47 Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHW581

   Plasmid

   #198818

   Purpose

   In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL-CRISPR.SFFV.PAC

   Plasmid

   #57829

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   SFFV
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-4mtD3cpv

    Plasmid

    #181874

    Purpose

    Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    4mtD3cpv
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    the 4mtD3cpv CDS differs from the recorded GeneBa…

    Promoter

    CaMKIIa

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_Xph15-GCaMP6f

    Plasmid

    #135538

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to GCaMP6f

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Use

    Tags

    GCaMP6f

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28_6xHis-PARP1-L713F

    Plasmid

    #174794

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L713F, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1-C125G

    Plasmid

    #174792

    Purpose

    Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-C125G (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C125G, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221-MICU1-mEF

    Plasmid

    #31765

    Depositor

    Vamsi Mootha

    Article

    Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

    Insert

    MICU1 (MICU1 Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Expression

    Mutation

    Mutated in both EF-hand domains: D231A, E242K, D4…

    Promoter

    Available since

    May 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FLS2-YFPc

    Plasmid

    #87166

    Purpose

    fluorescence complementation assay

    Depositor

    Silke Robatzek

    Article

    Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Use

    Tags

    YFPc

    Expression

    Mutation

    Promoter

    35s

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FLS2-YFPn

    Plasmid

    #87165

    Purpose

    fluorescence complementation assay

    Depositor

    Silke Robatzek

    Article

    Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Use

    Tags

    YFPn

    Expression

    Mutation

    Promoter

    35s

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-hPPP3CB-FLAG

    Plasmid

    #179135

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB-TetON-mEGFP-MYOD1_AroPERFECT

    Plasmid

    #215620

    Purpose

    For integration of MYOD1 AroPERFECT T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    MYOD1 AroPERFECT (MYOD1 Human)
    

    Use

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GlycophorinA-CD4d3+4-bio

    Plasmid

    #73093

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinA with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Glycophorin A (GYPA Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lactotransferrin-CD4d3+4-bio

    Plasmid

    #73124

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Lactotransferrin with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Lactotransferrin (LTF Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ICAM4-CD4d3+4-bio

    Plasmid

    #73102

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding ICAM4 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    ICAM4 (ICAM4 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CD55-CD4d3+4-bio

    Plasmid

    #73116

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding CD55 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    CD55 (CD55 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1(+)-FluoSTEP-AKAR

    Plasmid

    #181844

    Purpose

    Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-AKAR
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28_6xHis-PARP1-Y986H

    Plasmid

    #174801

    Purpose

    Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986H (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28_6xHis-PARP1-Y986S

    Plasmid

    #174802

    Purpose

    Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986S (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28_6xHis-PARP1-G972R

    Plasmid

    #174800

    Purpose

    Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G972R (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G972R, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ENDOD1-CD4d3+4-bio

    Plasmid

    #73114

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding ENDOD1 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    ENDOD1 (ENDOD1 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMRX-hSTING-dCTT

    Plasmid

    #214164

    Purpose

    Retroviral transduction of STING

    Depositor

    Nan Yan

    Article

    Wu et al J Exp Med. 2019 Apr 1;216(4):867-883. do

    Insert

    STING (dCTT) (STING1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    dCTT

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcdna DSG-2 TS

    Plasmid

    #89465

    Purpose

    DSG-2 tension sensor, using TSmod inserted in human DSG-2

    Depositor

    Daniel Conway

    Article

    Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

    Insert

    desmoglein-2 (DSG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AchE-CD4d3+4-bio

    Plasmid

    #73119

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding AchE with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    AchE (ACHE Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-Susd4(3.7)-EGFP-sv40

    Plasmid

    #184413

    Purpose

    AAV expression of tTA from mouse sushi domain containing 4 (Susd4) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Susd4 gene promoter-EGFP (Susd4 Mouse)
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Susd4 gene

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ERMAP-CD4d3+4-bio

    Plasmid

    #73107

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding ERMAP with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    ERMAP (ERMAP Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR221-MICU1-sh1-rORF

    Plasmid

    #31764

    Depositor

    Vamsi Mootha

    Article

    Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

    Insert

    MICU1 (MICU1 Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Expression

    Mutation

    RNAi-resistant ORF (rORF). 8 synonymous changes i…

    Promoter

    None

    Available since

    May 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. EGFP Rab11a S25N pcDNA3.1 Zeo (+)

    Plasmid

    #206080

    Purpose

    dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

    Depositor

    Annette Dolphin

    Article

    Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S25N

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EGFP Rab11a WT pcDNA3.1 Zeo (+)

    Plasmid

    #206079

    Purpose

    Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

    Depositor

    Annette Dolphin

    Article

    Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Lutheran-CD4d3+4-bio

    Plasmid

    #73101

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Lutheran with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Lutheran (BCAM Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. C1orf9-CD4d3+4-bio

    Plasmid

    #73109

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding C1orf9 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    C1orf9 (C1ORF9 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. DSCAML1-CD4d3+4-bio

    Plasmid

    #73105

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding DSCAML1 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    DSCAML1 (DSCAML1 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28_6xHis-PARP1delWGR-L713F

    Plasmid

    #173941

    Purpose

    Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delWGR-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 521-660; L713F, V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

    Plasmid

    #173866

    Purpose

    Mammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA5FRTTO-NHASt-codoptUS2

    Plasmid

    #135102

    Purpose

    Codon-optimized HSV-1 US2 with N-terminal HAStrep tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 US2 codon optimized
    

    Use

    Tags

    HA-Strep-Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AAV.CBA.YFP.miR-E_shStk16-1

    Plasmid

    #180391

    Purpose

    Producing AAV that encodes mouse Stk16 shRNA-1 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shStk16-1 (Stk16 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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43. pET28_6xHis-PARP1-A774L

    Plasmid

    #174796

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A774L (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A774L, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. pET28_6xHis-PARP1-W318R

    Plasmid

    #174793

    Purpose

    Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-W318R (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    W318, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pET28_6xHis-PARP1-A762V

    Plasmid

    #174795

    Purpose

    Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A762V (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A762V

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pcDNA5FRTTO-NHASt-codoptUL50

    Plasmid

    #135101

    Purpose

    Codon-optimized HSV-1 UL50 with N-terminal HAStrep tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 UL50 codon optimized
    

    Use

    Tags

    HA-Strep-Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

    Plasmid

    #173865

    Purpose

    Mammalian expression of mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

    Plasmid

    #63886

    Purpose

    An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized GCaMP3(wt)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human SYN1

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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49. pJ136

    Plasmid

    #29486

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Use

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Promoter

    Available since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

50. pcDNA5FRTTO-NHASt-codoptUL23

    Plasmid

    #135104

    Purpose

    Codon-optimized HSV-1 UL23 with N-terminal HAStrep tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 UL23 codon optimized
    

    Use

    Tags

    HA-Strep-Strep (N terminal on backbone)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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