We narrowed to 14,590 results for: pET

1. pET29b-IPP1-His

   Plasmid

   #124137

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Inorganic pyrophosphatase (IPP1 Budding Yeast)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-pAG-Hia5

   Plasmid

   #174373

   Purpose

   Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   protein AG - hia5
   

   Use

   Tags

   6xHis and Protein A/G

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-32a(+)-TGFB3

   Plasmid

   #120288

   Purpose

   Produce human recombinant TGFB3 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Transforming Growth Factor-β3 (TGFB3 Human, Synthetic)
   

   Use

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21a-GlnRS-His

   Plasmid

   #124108

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Glutaminyl-tRNA ligase (glnS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET263-pUC57 24xMS2V7

   Plasmid

   #140705

   Purpose

   MS2V7 cloning

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V7
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   24 MS2 loops V7, high-affinity C-variant, inter-s…

   Promoter

   None

   Available since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21a-SerRS-His

   Plasmid

   #124118

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Seryl-tRNA ligase (serS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET16b-His-ArgRS

   Plasmid

   #124104

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Argin+I4 ligase (argS Escherichia coli)
   

   Use

   Tags

   10x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21a-MetRS-His

   Plasmid

   #124115

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Methionine--tRNA ligase (metG Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21a-AspRS-His

   Plasmid

   #124106

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Aspartate-tRNA ligase (aspS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21a-His-IleRS

    Plasmid

    #124112

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Isoleucyl-tRNA ligase (ileS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21a-LeuRS-His

    Plasmid

    #124113

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Leucyl-tRNA ligase (leuS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21a-CysRS-His

    Plasmid

    #124107

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Cysteinyl-tRNA ligase (cysS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21a-GlyRS-His

    Plasmid

    #124110

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21a-ProRS-His

    Plasmid

    #124117

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Prolyl-tRNA ligase (proS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21a-TrpRS-His

    Plasmid

    #124120

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Tryptophanyl-tRNA ligase (trpS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21a-HisRS-His

    Plasmid

    #124111

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Histidyl-tRNA ligase (hisS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21a-TyrRS-His

    Plasmid

    #124121

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Tyrosyl-tRNA ligase (tyrS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a-ValRS-His

    Plasmid

    #124122

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Valyl-tRNA ligase (valS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETcon_SARS-CoV-2_RBD

    Plasmid

    #166782

    Purpose

    Yeast surface display vector, expressing the SARS-2 RBD, 201aa construct

    Depositor

    Jesse Bloom

    Article

    Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. d

    Insert

    Spike receptor binding domain (RBD) from SARS-CoV-2 (S )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21a-MTF-His

    Plasmid

    #124133

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Methionyl-tRNA formyltransferase (fmt Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET29b-AK1-His

    Plasmid

    #124135

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Adenylate kinase (AK1 Chicken)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28b-GBP-MTn

    Plasmid

    #154363

    Purpose

    Bacterial expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LOV-Turbo-His6_pET21a

    Plasmid

    #199668

    Purpose

    expresses LOV-Turbo in bacteria

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET15b-His-RF2

    Plasmid

    #124130

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Release factor 2 (prfB Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-28a (+)-hTBP

    Plasmid

    #171073

    Purpose

    Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    TATA-box-binding protein (TBP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BirA in pET28a (w400-2)

    Plasmid

    #26624

    Depositor

    Eric Campeau

    Insert

    BirA
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28b-DiCas7-11-His

    Plasmid

    #234482

    Purpose

    Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

    Insert

    DiCas7-11
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pETduet+sfGFP-TetR

    Plasmid

    #157794

    Purpose

    Expression of sfGFP-TetR fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    sfGFP-TetR
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET22b-CPDBamHI-Leu

    Plasmid

    #38256

    Depositor

    Matthew Bogyo, Aimee Shen

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pETDuet1_His-TEV-EGFP

    Plasmid

    #223723

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    EGFP, not monomeric (has A207)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28 His6-mEGFP-D4

    Plasmid

    #226372

    Purpose

    Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4 (pfoA Clostridium perfringens)
    

    Use

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pETM6-E12-vioABECD

    Plasmid

    #66537

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a-tdTomato-CNA35

    Plasmid

    #61606

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    tdTomato and CNA35
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-mCherry-CNA35

    Plasmid

    #61607

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mCherry

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mCherry and CNA35
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET246-pUC57 12xMS2V6

    Plasmid

    #104390

    Purpose

    MS2V6 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. ECFP-TevS-YPET

    Plasmid

    #100097

    Purpose

    FRET-based ECFP-YPET reporter that is cleaved by TEV

    Depositor

    Jim Wells

    Article

    Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

    Insert

    ECFP-TevS-YPET
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET-24a-ELP[V-30]

    Plasmid

    #67014

    Purpose

    Expresses ELP[V-30] in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    ELP[V-30]
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET22b-T7-6h-GST-α-Synuclein

    Plasmid

    #225224

    Purpose

    Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

    Inserts

    GST
    α-Synuclein
    

    Use

    Tags

    6xHis Tag and TEV Cleavage Site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET-28a-hPKM2

    Plasmid

    #44242

    Depositor

    Matthew Vander Heiden, Lewis Cantley

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

    Insert

    Pyruvate Kinase M2 (PKM Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET28a-MH6-Bsu

    Plasmid

    #163911

    Purpose

    Expresses Bsu large fragment for affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    Bsu polymerase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET14b – E. coli Exonuclease I

    Plasmid

    #104552

    Purpose

    bacterial expression of Exonuclease I

    Depositor

    Charles Bell

    Article

    Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

    Insert

    Exonuclease I (sbcB E.coli)
    

    Use

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET-28a-hPKM1

    Plasmid

    #44241

    Depositor

    Matthew Vander Heiden, Lewis Cantley

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

    Insert

    Pyruvate Kinase M1 (PKM Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET28c-F30-2xdBroccoli

    Plasmid

    #66843

    Purpose

    Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

    Insert

    Two dimeric Broccoli aptamers in the F30 scaffold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pETSXM2-pLacI-mtrCAB

    Plasmid

    #174618

    Purpose

    Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    mtrCAB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET28a-MH6-uvsY

    Plasmid

    #163914

    Purpose

    Expresses uvsY for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    uvsY (uvsY Enterobacteria phage T4)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET28_6xHis-PARP1-E988K

    Plasmid

    #173945

    Purpose

    Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-E988K (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A, E988K

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET30a-His6-LOXCAT

    Plasmid

    #140084

    Purpose

    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET21-pelB-LaM-4

    Plasmid

    #172770

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-4
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pET28a/Cas9-Cys

    Plasmid

    #53261

    Purpose

    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

    Depositor

    Hyongbum Kim

    Article

    Ramakrishna et al Genome Res. 2014 Apr 2.

    Insert

    Cas9-Cys
    

    Use

    Tags

    6X His tag, HA epitope, and NLS

    Expression

    Bacterial

    Mutation

    Additional Cysteine is added to the C-terminal re…

    Promoter

    T7 Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge