Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 15,547 results for: pET

Clear keywords
Showing: 1651 - 1700 of 15547 results

  6. pET30-GBF-E6

    Plasmid
    #21696
    Depositor
    James Baleja
    Article
    Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.
    Insert
    protein G domain B1 fused human papillomavirus type 16 amno acid residue 2-142 (E6 Human papillomavirus type 16)
    Tags
    Protein G B1 domain
    Expression
    Bacterial
    Mutation
    GB1 (1-56aa) fusion tag with mutations at three p…
    Available Since
    July 28, 2009
    Availability
    Academic Institutions and Nonprofits only

  14. pET-Trx-mSA2

    Plasmid
    #52320
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Article
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Tags
    FLAG, His, S tag, TEV site, and Thioredoxin
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available Since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52320-plasmid-map-sequence-id-84861

    Enlarge

  15. pET-MBP-mSA2

    Plasmid
    #52319
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Article
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Tags
    FLAG, His, MBP, and TEV
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available Since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52319-plasmid-map-sequence-id-237583

    Enlarge

  19. pET24a-VHH-myc

    Plasmid
    #109422
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, T7, and myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109422-plasmid-map-sequence-id-211585

    Enlarge

  22. pET15b His6-ecDHFR (WT)

    Plasmid
    #73188
    Purpose
    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)
    Depositor
    Thomas Wandless
    Insert
    folA dihydrofolate reductase (folA E. coli)
    Tags
    His 6X
    Expression
    Bacterial
    Mutation
    none
    Available Since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  44. pET-pAG-Hia5

    Plasmid
    #174373
    Purpose
    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein AG - hia5
    Tags
    6xHis and Protein A/G
    Expression
    Bacterial
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174373-plasmid-map-sequence-id-341861

    Enlarge

  45. pET-APOBEC1-YTH

    Plasmid
    #200158
    Purpose
    For bacterial expression of the APOBEC1-YTH fusion protein for protein purification
    Depositor
    Kate Meyer
    Article
    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882
    Insert
    APOBEC1-YTH
    Tags
    6xHis, HA, and Maltose Binding Protein
    Expression
    Bacterial
    Mutation
    Codon optimized for E coli expression
    Promoter
    LacZ
    Available Since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    200158-plasmid-map-sequence-id-396323

    Enlarge

  48. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

    Enlarge

  50. pET-TP1107(Q15TAG)

    Plasmid
    #247462
    Purpose
    Bacterial expression of anti-IgG VHH clone TP1107 with amber stop codon at Q15 for unnatural amino acid incorporation
    Depositor
    Angus Johnston
    Article
    Chen et al Nat Nanotechnol. 2025 Sep;20(9):1273-128
    Insert
    TP1107 Q15TAG
    Tags
    His6 and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Original Q15 codon (CAA) was modified to amber st…
    Promoter
    T7
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    247462-plasmid-map-sequence-id-494410

    Enlarge

Showing: 1651 - 1700 of 15547 results