173,520 results

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Level 1 P2 OsUbiP:Cas9:NosT

Plasmid

#165424

Purpose

GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

Os Ubiquitin promoter :: Cas9 :: NosTerminator

Use

Synthetic Biology

Promoter

Os Ubiquitin promoter :: Cas9 :: NosTerminator

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC mTagBFP2

Plasmid

#191566

Purpose

Expresses an shRNA and mTagBFP2

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-F-RAM-d2tTA-TRE-mKate2

Plasmid

#140274

Purpose

F-RAM, the Fos-dependent reporter

Depositor

Yingxi Lin

Article

Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

Insert

mKate2

Use

AAV

Promoter

TRE

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMK265 (RAD21-mAC Hygro)

Plasmid

#140539

Purpose

RAD21 tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-SUMO-MarathonRT

Plasmid

#109029

Purpose

Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

Depositor

Anna Pyle

Article

Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

Insert

ERE_01490

Tags

6xHis-SUMO tag

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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ATP5F1B-pmCherry

Plasmid

#232783

Purpose

Encodes an mCherry-tagged ATP5F1B

Depositor

Giulia Bertolin

Article

Sharma et al Cell Death Discov. 2023 Jun 29;9(1):203.

Insert

ATP5F1B (ATP5F1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLYS5-SDHB-Flag

Plasmid

#50055

Purpose

Expression of human SDHB in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

SDHB (SDHB Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pGY037 BSMV-gamma-AmCyan

Plasmid

#234818

Purpose

To express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA)

Depositor

Daniel Voytas

Article

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

AmCyan

Expression

Plant

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTP1122

Plasmid

#104159

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_OCT4

Plasmid

#26816

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-OCT4-3'UTR (POU5F1 Human)

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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ER-GCaMP6f

Plasmid

#182548

Purpose

Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Expression

Bacterial and Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTL032

Plasmid

#80928

Purpose

14x MS2 sense + 14x PP7 antisense with loxP-kanMX-loxP

Depositor

Daniel Larson

Article

Lenstra et al Mol Cell. 2015 Nov 19;60(4):597-610. doi

Insert

14xMS2 sense + 14xPP7 antisense, loxP-kanMX-loxP

Expression

Bacterial

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMMS22L-siR3

Plasmid

#221013

Purpose

mammalian expression vector of myc-Flag tagged MMS22L siRNA resistant

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

MMS22L (MMS22L Human)

Tags

Flag and myc

Expression

Mammalian

Mutation

silent mutations MMS22L: 5'-aaAacGtgTtgCtgTg…

Promoter

CMV

Available Since

July 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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HA-p62

Plasmid

#28027

Purpose

Mammalian expression of HA tagged human p62

Depositor

Qing Zhong

Article

Fan et al Autophagy. 2010 Jul 22. 6(5):.

Insert

p62 (SQSTM1 Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-IRES-BSD

Plasmid

#209751

Purpose

Lentiviral transfer plasmid to express the blasticidin resistance gene

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-Volton2-ST-WPRE

Plasmid

#172907

Purpose

AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Has Service

AAV1

Insert

Voltron2-ST

Use

AAV and Cre/Lox

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

hSynapsin1

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEQ276

Plasmid

#83945

Purpose

express human cytomegalovirus IE1 and IE2 genes in eukaryotic cells

Depositor

Adam Geballe

Article

Biegalke et al Virology. 1991 Jul;183(1):381-5.

Insert

IE1 and IE2

Expression

Mammalian

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-RYK-V5/HIS

Plasmid

#205610

Purpose

Expression of human RYK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

RYK receptor tyrosine kinase (RYK Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Level 1 P1 OsActinP:Hygint:NosT

Plasmid

#165423

Purpose

GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator

Use

Synthetic Biology; L1 p1(r) golden gate (moclo) h…

Promoter

Os Actin promoter :: Hygromycin resistance gene (…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pKlab003-pcDNA3.1-FKBP5-3xFLAG

Plasmid

#249006

Purpose

This plasmid expresses the C terminally 3x-FLAG tagged FKBP5 protein sequence for pulldown assays

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

FKBP5 (FKBP5 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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