165,567 results

1. HCT55_rAAV-eHGT453m_eHGT_1137m-minBGprom-SYFP2-WPRE3-bGHpA

   Plasmid

   #223945

   Purpose

   Direct-expressing SYFP2 AAV virus

   Depositor

   Tanya Daigle, Allen Institute for Brain Science

   Article

   Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

   Insert

   SYFP2
   

   Use

   AAV

   Mutation

   N/A

   Promoter

   minBglobin

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-HaloCKAR2.2-T2A-EGFP

   Plasmid

   #216493

   Purpose

   Expression of chemigenetic PKC biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloCKAR2.2
   

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-HaloAKAR2.1-CLC

   Plasmid

   #216492

   Purpose

   Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) targeted to clathrin coated pits

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.1-CLC
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRetro-CAG-DsRedExpress-TVA

   Plasmid

   #235699

   Purpose

   Control plasmid expressing TVA and DsRed without G protein

   Depositor

   Hongjun Song, Guo-li Ming

   Article

   Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

   Insert

   DsRedExpress, TVA800
   

   Use

   Retroviral

   Promoter

   CAG

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRetro-CAG-DsRedExpress-G-TVA

   Plasmid

   #235698

   Purpose

   Expresses G protein, TVA receptor, and DsRed for rabies virus trans-synaptic tracing

   Depositor

   Hongjun Song, Guo-li Ming

   Article

   Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

   Insert

   DsRedExpress, TVA800, and Glyco
   

   Use

   Retroviral

   Promoter

   CAG

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR_CMV_MeltTEVp-40-mCherry

   Plasmid

   #234064

   Purpose

   Encodes a TEV protease controlled by a Melt variant with a switching temperature of 40°C

   Depositor

   Lukasz Bugaj

   Article

   Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

   Insert

   MeltTEV-40-mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFAP-Flex-MCS-WPRE-SV40 - GCH32

   Plasmid

   #230830

   Purpose

   AAV cloning vector for CRE-dependent expression under the GFAP promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Cloning vector

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSR1154

   Plasmid

   #221653

   Purpose

   Tetracycline sensor v3 for Staphylococcus aureus

   Depositor

   Lauren Andrews

   Article

   Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

   Inserts

   sfGFP cassette
   TetR cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PXyl-TetO and PZwf

   Available Since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5/FRT-hMAPT

   Plasmid

   #231583

   Purpose

   Minigene of human MAPT containing exon 9, 10, 11 and relevant intronic sequences

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

   Insert

   human MAPT (MAPT Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR223-BPLF1_2001-3000

    Plasmid

    #198586

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BPLF1_2001-3000
    

    Use

    Gateway entry vector

    Mutation

    None (wildtype)

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF0251

    Plasmid

    #142574

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    VEZF1 (VEZF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR223-BPLF1_1-1000

    Plasmid

    #198582

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BPLF1_1-1000
    

    Use

    Gateway entry vector

    Mutation

    None (wildtype)

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ER-FAST-IRES-iRFP670

    Plasmid

    #216344

    Purpose

    For testing localization of pFAST and iRFP670 proteins to endoplasmic reticulum

    Depositor

    Alison Tebo

    Insert

    PRL_pFAST-IRES-CANX_iRFP670
    

    Tags

    myc for FAST and iRF670 proteins

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV6-AN-HA_aa 1-420 POLI

    Plasmid

    #201582

    Purpose

    Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Expresses amino acids 1-420 of DNA polymerase iota

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV6-AN-HA_WT POLI

    Plasmid

    #201440

    Purpose

    Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJM919_WT His POLI

    Plasmid

    #201444

    Purpose

    Low copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    lacIq

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJRM919_WT aa 1-419 His POLI

    Plasmid

    #201445

    Purpose

    Low copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLI

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Expresses amino acids 1-449 of DNA polymerase iot…

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR223-BPLF1_501-1500

    Plasmid

    #198583

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BPLF1_501-1500
    

    Use

    Gateway entry vector

    Mutation

    None (wildtype)

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR223-BPLF1_1001-2000

    Plasmid

    #198584

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BPLF1_1001-2000
    

    Use

    Gateway entry vector

    Mutation

    None (wildtype)

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR223-BPLF1_1501-2500

    Plasmid

    #198585

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BPLF1_1501-2500
    

    Use

    Gateway entry vector

    Mutation

    None (wildtype)

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge