We narrowed to 9,102 results for: sgRNA
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Plasmid#192205Purposelentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAsDepositorPublicationTypeEmpty backboneUseCRISPR, Lentiviral, and RNAiAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by
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SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA)
Plasmid#149278PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertModified TRV RNA2 with PEBV promoter and AtGly-tRNA mobile RNA sequence
UseT-dna vectorMutationT2318C (DNA) in PEBV coat protein sequenceAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
HeFSpCas9
Plasmid#92355PurposeExpression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, K848A, Q926A, K1003A, R1060APromoterCbhAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor
Plasmid#156432PurposeVector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCTCF, rtta3G, TetO3G (Ctcf Mouse)
UseMouse TargetingExpressionMammalianMutationnoneAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMonAID_dCas9-PmCDA_Hyg_ALS
Plasmid#91692PurposeMonocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAlsDepositorInsertSpCas9
TagsPmCDA1ExpressionPlantMutationD10A and H840A for dead Cas9Promoter2x35SAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAL11
Plasmid#261764PurposeEmpty backbone to clone sgRNA units with LacZ gene for blue white screeningDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceOct. 16, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2
Plasmid#199481PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorInsertGal4vp16/4xnrUAS-mKate2
UseCRISPRAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2
Plasmid#199482PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorInsertGal4vp16/4xnrUAS-mKate2
UseCRISPRAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD3-ovoD1
Plasmid#111142PurposeExpresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogasterDepositorAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCR-U6-gRNA-TRE3G-TdTomato
Plasmid#192658PurposeA plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomatoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTdTomato
ExpressionMammalianPromoterU6, TRE3GAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA EYFP
Plasmid#167906PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA EBFP2
Plasmid#167905PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgDonor_lentiGuide
Plasmid#184168PurposeLentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.DepositorInsertsgDonor
UseCRISPR and LentiviralExpressionMammalianAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWN_U6-TRAC-miniGag
Plasmid#228960PurposeminiEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertminiGag
UseCRISPRExpressionMammalianAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS156
Plasmid#182707PurposeCRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUF1-dCas9-Sir2a
Plasmid#122510PurposeCombining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-Sir2a
UseCRISPRTags3*FLAGMutationdCas9(D10A,H840A)PromoterPfHsp86Available sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mScarlet-TUBA1B
Plasmid#207764PurposeDonor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
UseCRISPR; Donor templateExpressionMammalianAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX330-EN1082_Rad21_STOP
Plasmid#156450PurposeFor transient expression of Cas9 and sgRNA targeting mouse RAD21 stop codonDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9, RAD21 (Rad21 Mouse)
UseCRISPRExpressionMammalianMutationnoneAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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