We narrowed to 8,596 results for: Set

1. pIRE4-Azi

   Plasmid

   #105829

   Purpose

   Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

   Depositor

   Irene Coin

   Article

   Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

   Inserts

   Humanized EAziRS
   4 copies of the U6-BstYam tRNA cassette
   

   Expression

   Mammalian

   Mutation

   (Y37L, D182S, F183M, L186A, D265R)TyrRS

   Promoter

   PGK and U6

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSH124-ssr

   Plasmid

   #198025

   Purpose

   Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   T0 terminator - Ap/Cb resistance cassette
   

   Expression

   Bacterial

   Promoter

   ApR promoter

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pR26 CAG AsiSI/MluI

   Plasmid

   #74286

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSH824-ssr

   Plasmid

   #198027

   Purpose

   Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   T0 terminator - Apr resistance cassette
   

   Expression

   Bacterial

   Promoter

   AprR promoter

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pR26 CAG/GFP Asc

   Plasmid

   #74285

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Mouse Targeting

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. v1em-Cterm-PE2max-U6-pegRNA

   Plasmid

   #198733

   Purpose

   AAV genome encoding C-terminal PE2max and U6 expression cassette

   Depositor

   David Liu

   Article

   Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

   Insert

   NpuC-CtermPE2max
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. v1em-Nterm-PE2max-U6-sgRNA

   Plasmid

   #198732

   Purpose

   AAV genome encoding N-terminal PE2max and U6 expression cassette

   Depositor

   David Liu

   Article

   Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

   Insert

   Nterm PE2max-NpuN
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGE347

   Plasmid

   #153228

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDA_526

    Plasmid

    #184859

    Purpose

    Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.

    Depositor

    Arlene Sharpe

    Article

    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

    Insert

    sgRNA cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDualEX-SMN2

    Plasmid

    #214431

    Purpose

    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN2 EXON 7 (SMN2 Human)
    

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGM65879

    Plasmid

    #153214

    Purpose

    Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassette

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDualIN-SMN2

    Plasmid

    #214430

    Purpose

    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN2 EXON 7 (SMN2 Human)
    

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGM65881

    Plasmid

    #153215

    Purpose

    Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNCS mOrange

    Plasmid

    #91764

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Orange (mOrange)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Orange
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-AAVS1-Ef1a-Puro

    Plasmid

    #227266

    Purpose

    Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJAT7

    Plasmid

    #204292

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-DsRed
    

    Use

    CRISPR

    Promoter

    3XP3

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMpGE_En03

    Plasmid

    #71535

    Purpose

    Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Takayuki Kohchi, Sumihare Noji

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-tdTomato

    Plasmid

    #159275

    Purpose

    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11

    Insert

    tdTomato
    

    Use

    AAV; S1 knockin donor vector

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSNA

    Plasmid

    #135192

    Purpose

    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    I-SceI lamba Red
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Promoter

    araBAD

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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