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pU6Rosa-CAG-Cas9
Plasmid#118998PurposeMammalian expression of Rosa26 sgRNA, Cas9 and BFPDepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
RV-Cag-Dio-mEYFP
Plasmid#87666Purposeretroviral vector encoding cre dependent expression of membrane EYFPDepositorInsertMem-EYFP
UseRetroviralTagspalmitylationExpressionMutationPromoterCAGAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Trex2-bpA
Plasmid#86984PurposeExpression of mouse Trex2DepositorInsertmouse Trex2 coding region (Trex2 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-Myc-ERas-IP
Plasmid#15690DepositorInsertERas (Eras Mouse)
UseTagsMyc (6x)ExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PrCas12a-2AeGFP
Plasmid#123636PurposeMammalian expression, Genome editingDepositorInsertPrCas12a
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3 (in pCAGGS)
Plasmid#118042PurposeGABAA receptor expression (gamma3 subunit) Myc-tag near N-terminusDepositorInsertGABRG3 (Gabrg3 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKCAG-mCherry-OptoJNKi
Plasmid#89738PurposeExpression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11DepositorInsertOptoJNKi
UseFluorescent proteinTagsmCherryExpressionMammalianMutationPromoterCAGAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-Otx2shRNA2
Plasmid#73982PurposeOtx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.DepositorInsertEGFP-Otx2-shRNA2
UseRNAiTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-Nanog(L122A)-IP
Plasmid#62992PurposeExpresses Mouse Nanog L122A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).DepositorInsertMouse Nanog L122A Mutant (Nanog Mouse)
UseTagsExpressionMammalianMutationChanged Leucine 122 (123 AA in Mouse Protein) to …PromoterAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-CrxshRNA
Plasmid#73987PurposeCrx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Crx gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BsCas12b-2AeGFP
Plasmid#121948PurposeMammalian expression, Genome editingDepositorInsertBsCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HDAC4 C-terminus
Plasmid#71400PurposeCAG promoter, mammalian expressionDepositorInsertHDAC4 C-terminus (HDAC4 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-S-501V2-FLAG
Plasmid#177096Purpose501V2 variant S-protein with c-terminal FLAG tagDepositorInsertS-protein 501V2 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CAG-EGFP-Otx2shRNA1
Plasmid#73981PurposeOtx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse Otx2 gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKCAG-H2BmCherry-OptoJNKi
Plasmid#89741PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11DepositorInsertOptoJNKi
UseOptogeneticsTagsHistone2B and mCherryExpressionMammalianMutationPromoterCAGAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-hCD4-GCaMP2
Plasmid#18932DepositorInserthCD4-GCaMP2 (CD4 Human)
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG/hArf4(WT)-EGFP
Plasmid#79407PurposeExpresses C-terminally EGFP-tagged Arf4(WT) in mammalian cellsDepositorInsertArf4 (ARF4 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-S-B.1.617.1-FLAG
Plasmid#177098PurposeB.1.617.1 variant S-protein with c-terminal FLAG tagDepositorInsertS-protein B.1.617.1 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-HDAC4 1-747
Plasmid#71399PurposeCAG promoter, mammalian expressionDepositorInsertHDAC4 1-747 (HDAC4 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-R-3xSV40pA-R-td-sfGFP
Plasmid#177150PurposeCAG tdGFP dre/rox reporter piggyBac transposon (3x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseDre / rox ; piggybacTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SRGAP3FBAR-EGFP
Plasmid#168506PurposeExpression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusionDepositorInsertSRGAP3-FBAR (SRGAP3 Human)
UseTagsEGFPExpressionMammalianMutationaa 1-492PromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AmCas12b-2AeGFP
Plasmid#121947PurposeMammalian expression, Genome editingDepositorInsertAmCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFPKir7.1
Plasmid#107182PurposeAdenoassociatedvirus particle generationDepositorInsertKCNJ13 (KCNJ13 Human)
UseAAVTagsEGFPExpressionMutationPromoterCAGAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFrt-invCAG-Luc
Plasmid#63577PurposeShuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sitesDepositorInsertStop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
UseCre/Lox; Frt/flpe recombination mediated insertionTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TetR-RNF169UBD
Plasmid#119011PurposeMammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expressionDepositorInsertTetR
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-MosIR
Plasmid#120516PurposedsRNA expressionDepositorInsertMos (Mos Mouse)
UseRNAiTagsExpressionMutationL170P in second MosIR repeat (see comments below)PromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.S72A
Plasmid#106188PurposeWeak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.S72A
UseAAVTagsExpressionMutationGltI: S72APromoterCAGAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG aconitase GFP
Plasmid#66104Purposeexpression of chicken aconitase GFPDepositorInsertaconitase
UseChicken expressionTagsEGFPExpressionMutationPromoterCAGAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
RV-Cag-Dio-mTdt
Plasmid#87665Purposeretroviral vector encoding cre dependent expression of membrane TdtomatoDepositorInsertMem-Tdtomato
UseRetroviralTagsExpressionMutationPromoterCAGAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only
