We narrowed to 4,347 results for: ara-2

1. pVH015

   Plasmid

   #80398

   Purpose

   Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots

   Depositor

   Richard Murray

   Article

   Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

   Inserts

   Anti-scaffold-ffGFP-3xFLAG
   CusR (cusR )
   AraC (araC )
   

   Use

   Synthetic Biology

   Tags

   C-terminal 3xFLAG tag and C-terminal LZx domain

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDGB1alpha11_SulR

   Plasmid

   #186424

   Purpose

   transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assembly

   Depositor

   Lukas Fischer

   Article

   Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

   Insert

   SulR
   

   Use

   Synthetic Biology; Binary vector for escherichia …

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDGB1alpha2_KanR_BastaR_Rb7_GFP

   Plasmid

   #186427

   Purpose

   optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

   Depositor

   Lukas Fischer

   Article

   Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

   Inserts

   KanR
   BastaR
   Rb7
   eGFP
   

   Use

   Synthetic Biology; Binary vector for escherichia …

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   CaMV 35S and Pnos

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-synP-FLEX-splitTVA-EGFP

   Plasmid

   #52474

   Purpose

   Expresses splitTVA-EGFP

   Depositor

   Ian Wickersham

   Article

   Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

   Insert

   splitTVA-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin-1

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBG-ttLbUV2

   Plasmid

   #231386

   Purpose

   LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.

   Depositor

   Qi-Jun Chen

   Article

   Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

   Insert

   ttLbCas12a Ultra V2
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D156R and E795L

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-synP-FLEX-splitTVA-EGFP-B19G

   Plasmid

   #52473

   Purpose

   Expresses splitTVA-EGFP-B19G

   Depositor

   Ian Wickersham

   Article

   Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

   Has Service

   AAV1

   Insert

   TVA-P2A-EGFP-P2A-B19G
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin-1

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCherry-7aa-ActA-pEGFP-C1

   Plasmid

   #177406

   Purpose

   To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.

   Depositor

   David Andrews

   Article

   Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

   Insert

   ActA tail anchor (actA Listeria monocytogenes)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUBQ10:AtFLS2

   Plasmid

   #202186

   Purpose

   Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter

   Depositor

   Gitta Coaker

   Article

   Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

   Insert

   Flagellin sensing 2 (FLS2 Mustard Weed)
   

   Use

   Tags

   HA

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 7M8

   Plasmid

   #64839

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

   Depositor

   John Flannery, David Schaffer

   Article

   Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

   Insert

   7M8 cap
   

   Use

   AAV

   Tags

   Expression

   Mutation

   7mer insertion in the AAV2 cap

   Promoter

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3 HRPT2 136X

    Plasmid

    #11049

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 136X (CDC73 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncation mutant with a stop codon introduced at…

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3 HRPT2 227X

    Plasmid

    #11050

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 227X (CDC73 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncation mutant with a stop codon introduced at…

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pCAGGS-RaichuEV-Rac1(T17N)

    Plasmid

    #164902

    Purpose

    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    RaichuEV-Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T17N mutation in the Rac1 domain which make the s…

    Promoter

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28-His8-aCat(660-906)

    Plasmid

    #203333

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-906 only

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28-His8-aCat(660-865)

    Plasmid

    #203335

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-865 only

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-His8-aCat(660-885)

    Plasmid

    #203334

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-885 only

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pROS13

    Plasmid

    #107927

    Purpose

    kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DNMT3ACD-CRY2-EGFP

    Plasmid

    #82556

    Purpose

    Encodes DMNT3 under LITE2.0 system

    Depositor

    Joseph Irudayaraj

    Article

    Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

    Inserts

    DNMT3A (DNMT3A Human)
    CRY2 (CRY2 Mustard Weed)
    EGFP
    

    Use

    AAV

    Tags

    2A, NLS(alpha-imp), and NLS-VP64

    Expression

    Mammalian

    Mutation

    CRY2PHR (N-terminal photolyase homology region do…

    Promoter

    EF-1alpha

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXs-Puro-ARHGAP26

    Plasmid

    #69467

    Purpose

    Expresses ARHGAP26

    Depositor

    Axel Hillmer

    Article

    Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

    Insert

    ARHGAP26 (ARHGAP26 Human)
    

    Use

    Retroviral

    Tags

    HA TAG

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET15b-At 14-3-3 nu

    Plasmid

    #44940

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 nu (GRF7 Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pET15b-At 14-3-3 epsilon

    Plasmid

    #44936

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 epsilon (GRF10 Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

21. H6_fold-Z _Gogy

    Plasmid

    #201828

    Purpose

    Expresses H6_fold-Z _Gogy in bacterial cells

    Depositor

    Nobuyasu Koga

    Article

    Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

    Insert

    H6_fold-Z _Gogy
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. H7_fold-K _Mussoc

    Plasmid

    #201830

    Purpose

    Expresses H7_fold-K _Mussoc in bacterial cells

    Depositor

    Nobuyasu Koga

    Article

    Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

    Insert

    H7_fold-K _Mussoc
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. H6_fold-U _Nomur

    Plasmid

    #201829

    Purpose

    Expresses H6_fold-U _Nomur in bacterial cells

    Depositor

    Nobuyasu Koga

    Article

    Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

    Insert

    H6_fold-U _Nomur
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. H6_fold-C _Rei

    Plasmid

    #201827

    Purpose

    Expresses H6_fold-C _Rei in bacterial cells

    Depositor

    Nobuyasu Koga

    Article

    Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

    Insert

    H6_fold-C _Rei
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. KA1153_pPBCAG-MerCreMer-IN

    Plasmid

    #124183

    Purpose

    piggyBac-based expression of the tamoxifen-inducible Cre

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    MerCreMer-IRES-Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-SARS-Spike

    Plasmid

    #145031

    Purpose

    Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV Spike
    

    Use

    Tags

    C9 and CD5 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pSLS.492

    Plasmid

    #184957

    Purpose

    Test effect of a1/a2 length on editing rpoB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    rpoB donor T1534C, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pK031.TRE-Cre (Supernova)

    Plasmid

    #69136

    Purpose

    For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

    Depositor

    Takuji Iwasato

    Article

    Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

    Insert

    NLS-Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pENT-L1-Kozak-lacZ-stop-L2

    Plasmid

    #186359

    Purpose

    Entry clone with ORF encoding lacZ flanked by Gateway recombination sequences.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ Synthetic, E. coli)
    

    Use

    Expression of lacz reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHAGE-CMV-Rheb-IRES-eGFP-W

    Plasmid

    #32519

    Depositor

    Mustafa Sahin

    Article

    Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

    Insert

    Rheb (Rheb Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pCI pHluorin Syt2

    Plasmid

    #184503

    Purpose

    Mammalian expression of Synaptotagmin 2 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 2 (Syt2 Mouse)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. UMG-SP-2_pET29b

    Plasmid

    #215434

    Purpose

    Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. KA2628_pGL4-UBCmin-luc2/Tbx3-B'

    Plasmid

    #124201

    Purpose

    Luciferase reporter driven by the Tbx3 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Tbx3 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pZ001 (AacC2c1 locus)

    Plasmid

    #70166

    Purpose

    Expresses AacC2c1 full locus

    Depositor

    Feng Zhang

    Article

    Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

    Insert

    AacC2c1 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pZ003 (LshC2c2 locus)

    Plasmid

    #70168

    Purpose

    Expresses LshC2c2 full locus

    Depositor

    Feng Zhang

    Article

    Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

    Insert

    LshC2c2 full locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSCL.028

    Plasmid

    #184965

    Purpose

    Test effect of extending -Eco1 a1/a2 in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(extended), a1/a2 length: 27
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    human codon optimized RT, a1/a2 length extended t…

    Promoter

    Gal7

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Dapp1-PH pET11a

    Plasmid

    #71376

    Purpose

    Phosphoinositide Binding

    Depositor

    Mark Lemmon

    Article

    Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

    Insert

    Dapp1-PH (DAPP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 148-273

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

38. KA1158_pPBCAG-MerCreMer-IZ

    Plasmid

    #124184

    Purpose

    piggyBac-based expression of the tamoxifen-inducible Cre

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    MerCreMer-IRES-Zeo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pmPB3-VSV

    Plasmid

    #68039

    Purpose

    Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B3 (Plxnb3 Mouse)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. KA1875_pGL4-UBCmin-luc2/Sox2_SRR2

    Plasmid

    #124198

    Purpose

    Luciferase reporter driven by the Sox2 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Sox2 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge