We narrowed to 8,601 results for: Ott

1. pAB31 (punc-47::GtACR2::mCerulean::bHK::Chrimson)

   Plasmid

   #214891

   Purpose

   Expresses the optogenetic tool BiPOLES in GABAergic neurons of C. elegans.

   Depositor

   Alexander Gottschalk

   Article

   Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

   Insert

   GtACR2_mCerulean_bHK_Chrimson
   

   Use

   Tags

   mCerulean

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCA528 huPQBP1

   Plasmid

   #217449

   Purpose

   Expresses full-length human PQBP1 from a His-SUMO bacterial expression vector

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   PQBP1 (PQBP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HIV-1-GFP ΔEnv CA E45A

   Plasmid

   #217438

   Purpose

   HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   gag/pol
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   CA E45A

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HIV-1-GFP ΔEnv CA M66I

   Plasmid

   #217440

   Purpose

   HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   gag/pol
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   CA M66I

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SG3ΔENV CA Q67H/N74D

   Plasmid

   #217442

   Purpose

   Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA Q67H/N74D).

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   gag/pol
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   CA Q67H,N74D

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A

   Plasmid

   #204622

   Purpose

   Single-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Insert

   mRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. phABCA3-eGFP_CRISPR_Donor

   Plasmid

   #188540

   Purpose

   Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

   Depositor

   Darrell Kotton

   Article

   Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

   Insert

   EGFP
   

   Use

   CRISPR; Donor for homologous recombination

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLC-Flag-SENP8 sg2R-P2A-Hygro

   Plasmid

   #124300

   Purpose

   Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   SENP8 (SENP8 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   several silent mutations to disrupt sgRNA targeti…

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEMS2046

   Plasmid

   #79654

   Purpose

   ssAAV genome with Ple257 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

   Insert

   ssAAV Ple257-EmGFP WPRE
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   PAX6 Enhancer

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHCD1E

    Plasmid

    #102956

    Purpose

    gene cloning of human CD1E

    Depositor

    Gennaro De Libero, Lucia Mori

    Article

    Facciotti et al Proc Natl Acad Sci U S A. 2011 Aug 23;10

    Insert

    CD1E cDNA (CD1E Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEMS1930

    Plasmid

    #79779

    Purpose

    High copy number pUC vector containing Ple257 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple257
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple257 (PAX6 MiniPromoter)

    Available since

    Nov. 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pEMS2047

    Plasmid

    #79655

    Purpose

    ssAAV genome with Ple258 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    ssAAV Ple258-EmGFP WPRE
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    PAX6 Enhancer

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEMS1931

    Plasmid

    #79780

    Purpose

    High copy number pUC vector containing Ple258 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple258
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple258 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pEMS1929

    Plasmid

    #79778

    Purpose

    High copy number pUC vector containing Ple256 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple256
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple256 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pEMS1928

    Plasmid

    #79777

    Purpose

    High copy number pUC vector containing Ple255 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple255
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple255 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pEMS1927

    Plasmid

    #79776

    Purpose

    High copy number pUC vector containing Ple254 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple254
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple254 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. 213-petDuet1-GBD-LUMP--cdc42

    Plasmid

    #65973

    Purpose

    Dual expression of GBD-LUMP and cdc42

    Depositor

    Gerard Marriott

    Article

    Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

    Inserts

    LUMP
    cell division cycle 42 (CDC42 Human)
    

    Use

    Tags

    GBD (GTPase binding domain from ACK1; residues 44…

    Expression

    Bacterial

    Mutation

    constitutively active Cdc42 (Q61L)

    Promoter

    T7

    Available since

    July 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVSV-G

    Plasmid

    #138479

    Purpose

    Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    VSV-G envelop protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-AmCyan

    Plasmid

    #138481

    Purpose

    For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    AmCyan
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti6/V5-DEST-HSF1

    Plasmid

    #31210

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    HSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE

    Plasmid

    #236242

    Purpose

    Lentiviral expression of SpCas9

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    SpCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5-FRT/TO-eGFP-53BP1

    Plasmid

    #60813

    Purpose

    mammalian expression vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

    Plasmid

    #155305

    Purpose

    Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
    

    Use

    Lentiviral

    Tags

    2xNLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126706

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

    Plasmid

    #216477

    Purpose

    lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    dsRed
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1aL and UBC

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFB2E

    Plasmid

    #195288

    Purpose

    Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK EGFP 2A Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHAGE-FGFR3__TACC3

    Plasmid

    #116818

    Purpose

    Lentiviral expression of FGFR3__TACC3 fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR3__TACC3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFB2D

    Plasmid

    #195286

    Purpose

    Bicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR EGFP 2A Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti6/V5-DEST-FASCIN

    Plasmid

    #31207

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    FASCIN (FSCN1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FLAG-Rheb (Q64L)-pcw107

    Plasmid

    #64607

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    RHEB (RHEB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Q64L

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

31. FLAG-IKKbeta (S177E,S181E)-pcw107

    Plasmid

    #64609

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    IKBKB
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    S177E, S181E

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

    Plasmid

    #155307

    Purpose

    Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF1a

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHAGE-GTF2IRD1__ALK

    Plasmid

    #116821

    Purpose

    Lentiviral expression of GTF2IRD1__ALK fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    GTF2IRD1__ALK
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LCV2 IRF3 KO

    Plasmid

    #217446

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human IRF3

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    sgRNA targeting IRF3 (IRF3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HA-MDFIC

    Plasmid

    #207835

    Purpose

    Tagged MDFIC for western blotting and imaging

    Depositor

    Charles Cox

    Article

    Zhou et al Science. 2023 Aug 18;381(6659):799-804.

    Insert

    MDFIC (MDFIC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSEMS-Tom20-mEGFP-reHaloTagF

    Plasmid

    #200190

    Purpose

    Tom20 with reversible HaloTagF variant for fluorescent labeling

    Depositor

    Jacob Piehler

    Article

    Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

    Insert

    Tom20 (TOMM20 Human)
    

    Use

    Tags

    mEGFP and reHaloTagF

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. FLAG-Mkk7-JNK2 fusion-pcw107

    Plasmid

    #64618

    Purpose

    Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    Map2k7 linker MAPK9
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    mouse-human fusion

    Promoter

    PGK

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pLC-Flag-CSNK1A1 WT-Puro

    Plasmid

    #123319

    Purpose

    Lentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLenti6/V5-DEST-HMGB1

    Plasmid

    #31208

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pHAGE-EF1aL-WT_ABCA3-GFP

    Plasmid

    #188548

    Purpose

    Lentiviral vector allowing for EF1aL-driven constitutive expression of wildtype human ABCA3:GFP fusion protein

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    ABCA3:GFP (ABCA3 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1aL

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pHAGE-ALDH5A1__MAP3K5

    Plasmid

    #116807

    Purpose

    Lentiviral expression of ALDH5A1__MAP3K5 fusion

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ALDH5A1__MAP3K5
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLenti6/V5-DEST-MCM7

    Plasmid

    #31212

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    MCM7 (MCM7 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

43. psd44-iGFP-MFGE8-long

    Plasmid

    #46847

    Purpose

    expression of MFGE8 in mammalian cells

    Depositor

    Agnese Mariotti

    Article

    Carrascosa et al Oncogene. 2012 Mar 22;31(12):1521-32. do

    Insert

    MFGE8 (Mfge8 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK promoter

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

44. myr-FLAG-PIK3CA-pcw107

    Plasmid

    #64605

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    PIK3CA (PIK3CA Bovine)
    

    Use

    Lentiviral

    Tags

    FLAG and myristoylated

    Expression

    Mutation

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

45. pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE

    Plasmid

    #122056

    Purpose

    Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253P

    Depositor

    Stephen Tapscott

    Article

    Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

    Insert

    MD(ND2bHLH)WCS (Myod1, Neurod2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    MyoD chimeric protein substituted with the NeuroD…

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pOPINB-hRNF216(511-784)

    Plasmid

    #171923

    Purpose

    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.

    Depositor

    Bernhard Lechtenberg

    Article

    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

    Insert

    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    

    Use

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    codon optimised for expression in E. coli

    Promoter

    T7

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. PL-5LTR-GW-A

    Plasmid

    #138480

    Purpose

    For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. AP1muA single guide RNA

    Plasmid

    #230030

    Purpose

    guide RNA for AP1muA tagging at the C-terminus in pX459

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

    Insert

    AP1M1 guide (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126698

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

    Insert

    sgRNA targeting human SCGB3A2 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pLenti6/V5-DEST-ANLN

    Plasmid

    #31203

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    ANLN (ANLN Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only