159,557 results

1. PLKO.1-Scrambled

   Plasmid

   #136035

   Purpose

   Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   None (Scrambled)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZac2.1-GfaABC1D -IgK-TurboID-HA

   Plasmid

   #219810

   Purpose

   Secreted HA-tagged TurboID for extracellular proximity labeling

   Depositor

   Cagla Eroglu

   Article

   Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

   Insert

   IgK (Igk Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLAMP1-miRFP670nano

   Plasmid

   #127435

   Purpose

   LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   LAMP1-miRFP670nano
   

   Use

   Tags

   miRFP670nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-CD80

   Plasmid

   #223603

   Purpose

   Lentiviral expression of human CD80 in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD80 (CD80 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_hSyn_WiChR_TS_mScarlet_ER

   Plasmid

   #205997

   Purpose

   High efficient, K+ selective channelrhodopsin for the inhibition of neurons

   Depositor

   Peter Hegemann, Johannes Vierock

   Article

   Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

   Insert

   Wobblia Inhibitory Channelrhodopsin
   

   Use

   AAV

   Tags

   TS, mScarlet, ER

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. lvl0 pOp6-35S

   Plasmid

   #153409

   Purpose

   Dex system inducible promoter

   Depositor

   Naomi Nakayama

   Article

   Andreou et al bioRxiv, March 31, 2021

   Insert

   pOp6-35S promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGTR

   Plasmid

   #63143

   Purpose

   PCR template to construct polycistronic tRNA-gRNA

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Insert

   gRNA-tRNA fusion
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cys-3HB-SPYcatcher

   Plasmid

   #214838

   Purpose

   This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   Cys-3HB-SPYcatcher
   

   Use

   Tags

   His-TEV-SUMO-

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF1906

   Plasmid

   #141883

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TCF7L2 (TCF7L2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRetroX SgK269 WT

    Plasmid

    #68028

    Purpose

    expression of Sgk269 in mammalian cells

    Depositor

    Roger Daly

    Article

    Croucher et al Cancer Res. 2013 Mar 15;73(6):1969-80. d

    Insert

    PEAK1 (PEAK1 Human)
    

    Use

    Retroviral

    Tags

    FLAG/myc, HA, and IRES-DsRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-HA2-ROC1

    Plasmid

    #19897

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    RING finger protein 1 (ROC1) (RBX1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG mito-GCaMP5G

    Plasmid

    #105009

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    GCaMP5G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG/ATP8B4-HA

    Plasmid

    #209212

    Purpose

    Mammalian expression of ATP8B4

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP8B4 (ATP8B4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. RCAS-Flag-BRAFV600E

    Plasmid

    #126615

    Purpose

    Retroviral expression of Flag tagged human oncogenic BRAFV600E

    Depositor

    Yi Li

    Article

    Bu et al Cancer Res. 2019 Jan 1;79(1):61-71. doi:

    Insert

    BRAF (BRAF Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    V600E

    Promoter

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCBB-eGFP

    Plasmid

    #32548

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. paavCAG-iCre

    Plasmid

    #51904

    Purpose

    For AAV expression of iCre recombinase

    Depositor

    Jinhyun Kim

    Article

    Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

    Insert

    improved Cre recombinase
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ai6

    Plasmid

    #22798

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

    Insert

    CAG-Floxed ZsGreen
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. HiBiT-FZD8

    Plasmid

    #195846

    Purpose

    Expresses human FZD8 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD8 (FZD8 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AiP13999 - pAAV-AiE0387m-minBG-iCre(R297T)-BGHpA (Alias: CN3999)

    Plasmid

    #208166

    Purpose

    AiE0387m is an enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Insert

    iCreR297T
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

    Plasmid

    #111393

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis, Myc, T7, and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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