We narrowed to 16,821 results for: IGH@

1. pEVOL-NBK-1

   Plasmid

   #207639

   Purpose

   A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The evolved synthetase NBK-1 contains mutations Y…

   Promoter

   araBAD

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR-p38KTRmCerulean3

   Plasmid

   #59149

   Purpose

   ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HPF1-c004

   Plasmid

   #216874

   Purpose

   Bacterial expression vector for E. coli producing full length HPF1 protein with N-terminal YFP fusion

   Depositor

   Lari Lehtiö

   Article

   Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

   Insert

   Full length HPF1 (HPF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CRISPR-StAR1 (Retro)

   Plasmid

   #222687

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   PGK without BbsI cutsite

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CRISPR-StAR2 (Retro)

   Plasmid

   #222688

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   PGK without BbsI cutsite

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CRISPR-StAR3 (Retro)

   Plasmid

   #222689

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   PGK without BbsI cutsite

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRExT2A-CT-PmSc

   Plasmid

   #232549

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-PAC-3xTy

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV CAG-DO-TC66T-mCherry

   Plasmid

   #175178

   Purpose

   Cre-off TVA-mCherry fusion protein with 66T mutation

   Depositor

   Bernardo Sabatini

   Article

   Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

   Insert

   TVA
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   E66T

   Promoter

   CAG

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mTagBFP2-MAPTau-N-10

   Plasmid

   #55312

   Purpose

   Localization: Microtubules, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   MAPTau (MAPT Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. TRE3G-CTCF-dRBR-HA-MND-zeo

    Plasmid

    #232802

    Purpose

    Inducible over expression of RNA-binding domain deficient form of CTCF.

    Depositor

    Chunliang Li

    Article

    Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

    Insert

    Ctcf-dRBR (Ctcf Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PresentER-NLVPMVATV (GFP)

    Plasmid

    #102947

    Purpose

    PresentER minigene encoding HLA-A*02:01 ligand NLVPMVATV (GFP)

    Depositor

    David Scheinberg

    Article

    Gejman et al Cancer Immunol Res. 2020 May;8(5):672-68

    Insert

    NLVPMVATV
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mTagBFP2-CD151-7

    Plasmid

    #55282

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    CD151 (CD151 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pTPH413

    Plasmid

    #185728

    Purpose

    Bacterial PAM profiling of adenine base editor variants

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Inserts

    ecTadA(8e)-neNme2-C
    Nme2Cas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. B2342_BTB-mCherry

    Plasmid

    #215095

    Purpose

    Expresses BCL6 BTB(1-129)-mCherry in mammalian cells

    Depositor

    Chandra Tucker

    Article

    Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

    Insert

    BCL6 BTB (BCL6 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)

    Plasmid

    #193854

    Purpose

    Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    5' Cbr-unc-119(+) + 5' mNeonGreen
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Cbr-unc-119p

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-mNeptune2

    Plasmid

    #51309

    Purpose

    Expresses far-red fluorescent protein mNeptune2 in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mNeptune2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJ609-dCas9-KR-puro

    Plasmid

    #158478

    Purpose

    Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells

    Depositor

    Brendan Price

    Article

    House et al PLoS One. 2020 Dec 17;15(12):e0237759. d

    Insert

    dCas9-KR
    

    Use

    CRISPR

    Tags

    KillerRed

    Expression

    Mammalian

    Mutation

    Cas9m4 (D10A+D839A+H840A+N863A)

    Promoter

    CMV

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tdp43-EGFP construct8

    Plasmid

    #28201

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa275-313 deleted

    Promoter

    Available since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pGL3-MTS-G3635A-L17-Q2L7-T2176C-UGI-Puro

    Plasmid

    #209646

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-Q2L7-T2176C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-MTS-G3635A-L17-FZY2-S100N-UGI-Puro

    Plasmid

    #209642

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-FZY2-S100N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL3-MTS-G3635A-L17-FZY2-S100C-UGI-Puro

    Plasmid

    #209641

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-FZY2-S100C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAVS1-SA-neo-CAGGS-PE2-2A-GFP

    Plasmid

    #180014

    Purpose

    Targeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locus

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    PE2-2A-GFP
    

    Use

    CRISPR; Human genome engineering

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-DIO-Ef1a-TVA-2A-oG

    Plasmid

    #172359

    Purpose

    Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling specifically from cre-expressing neurons

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-oG
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Human HUWE1 (3993-4374)

    Plasmid

    #110754

    Purpose

    Bacterial Expression of HUWE1 HECT domain

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lenti-LbCpf1-Blast

    Plasmid

    #84751

    Purpose

    Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    LbCpf1
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-3xHA (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mTagRFP-T-Clathrin-15

    Plasmid

    #58005

    Purpose

    Localization: Clathrin Vesicles, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Clathrin (CLTB Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pJT130_GalL_nCDA1-NGBE3

    Plasmid

    #145094

    Purpose

    Expressing base editor nCDA1-NGBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-NGBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-D2s-L-Venus

    Plasmid

    #19966

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-monomeric Venus (DRD2 Aequorea Victoria, Human)
    

    Use

    Tags

    Flag and monomeric Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-FRT-EF1a-TVA-2A-N2cG

    Plasmid

    #172361

    Purpose

    Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-N2cG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pProEx M-Ras(G22C) CL

    Plasmid

    #224268

    Purpose

    Protein purification of M-Ras (G22C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    M-Ras (MRAS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G22C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge