Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 22,696 results for: Sis;

Showing: 10651 - 10680 of 22696 results

  1. pBAD24-rnc-sfGFP

    Plasmid
    #51560
    Purpose
    Gene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).
    Depositor
    Francisco Malagon, Sankar Adhya
    Article
    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261
    Insert
    rnc (rnc E. coli)
    Use
    Tags
    GLESTCRHASLAVLADERRFSA and superfolder GFP
    Expression
    Bacterial
    Mutation
    Contains additonal aa at the end of the sfGFP in …
    Promoter
    Available Since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    51560-plasmid-map-sequence-id-78838

    Enlarge

  2. pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)

    Plasmid
    #47387
    Purpose
    allow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectin
    Depositor
    Henry Lester
    Article
    Frazier et al J Biol Chem. 2013 May 29.
    Insert
    glutamate-gated chloride Channels
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Leu9 prime mutated to F (Phenylalanine in M3-M4 l…
    Promoter
    CMV
    Available Since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    47387-plasmid-map-sequence-id-67454

    Enlarge

  5. pCGN-ELK-1 S383A

    Plasmid
    #27160
    Purpose
    For mammalian expression of human Elk-1 with an HA tag and a mutation in the MAPK phosphorylation site of the transcriptional activation domain
    Depositor
    Ron Prywes
    Article
    Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-
    Insert
    ELK1 (ELK1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    TAD mutant: S383A
    Promoter
    Available Since
    Jan. 25, 2011
    Availability
    Academic Institutions and Nonprofits only
    27160-plasmid-map-sequence-id-322018

    Enlarge

  8. pCB-CBEv2

    Plasmid
    #221139
    Purpose
    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    lacIq-Ptac
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221139-plasmid-map-sequence-id-437031

    Enlarge

  10. pDGE479

    Plasmid
    #153270
    Purpose
    M4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153270-plasmid-map-sequence-id-307108

    Enlarge

  20. pHelper-CBE-sgRNA

    Plasmid
    #221137
    Purpose
    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with Cas9 handle
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221137-plasmid-map-sequence-id-436076

    Enlarge

  22. pTRIPZ-Myr-PTEN

    Plasmid
    #206983
    Purpose
    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signal
    Depositor
    Ming Chen
    Article
    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:
    Insert
    PTEN (PTEN Human)
    Use
    Lentiviral
    Tags
    N-terminal myristoylation site
    Expression
    Mammalian
    Mutation
    Promoter
    tet-on promoter
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206983-plasmid-map-sequence-id-408428

    Enlarge

  24. ABI_cPPVp

    Plasmid
    #119212
    Purpose
    Expresses C-terminus of plum pox virus protease (PPVp) fused to the ABI-abscisic acid responsive protein domain
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cPPVp in fusion with ABI
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119212-plasmid-map-sequence-id-231816

    Enlarge

  25. PYL1_nPPVp

    Plasmid
    #119211
    Purpose
    Expresses N-terminus of plum pox virus protease (PPVp) fused to PYL1-abscisic acid responsive protein domain
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nPPVp in fusion with PYL1
    Use
    Tags
    AU1 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119211-plasmid-map-sequence-id-232035

    Enlarge

  30. pLenti-gRNA-puro

    Plasmid
    #180426
    Purpose
    Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.
    Depositor
    Moshe Szyf
    Article
    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:
    Insert
    gRNAscr
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    180426-plasmid-map-sequence-id-354805

    Enlarge

Showing: 10651 - 10680 of 22696 results