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167,150 results

Showing: 1051 - 1080 of 167150 results

  3. pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4

    Plasmid
    #193294
    Purpose
    episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    mCherry-t2a-SOX2-17-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available Since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    193294-plasmid-map-sequence-id-379650

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  12. pSpCas9(BB)-2A-Hygro

    Plasmid
    #127763
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127763-plasmid-map-sequence-id-247560

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  13. pT4-CMV-GFP

    Plasmid
    #117046
    Purpose
    Optimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoter
    Depositor
    Wolfgang Uckert
    Insert
    GFP
    Use
    Transposon
    Promoter
    CMV
    Available Since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    117046-plasmid-map-sequence-id-258678

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  15. pAAV-hSyn-hM3D(Gq)-mCherry

    Plasmid
    #50474
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50474-plasmid-map-sequence-id-471606

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  17. pcDNA3.1-(-)-Pur

    Plasmid
    #200458
    Purpose
    Eukaryotic expression vector
    Depositor
    Bertrand Collet
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200458-plasmid-map-sequence-id-394118

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  20. MCS-13X Linker-BioID2-HA

    Plasmid
    #80899
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    80899-plasmid-map-sequence-id-181185

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  22. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid
    #185911
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185911-plasmid-map-sequence-id-367364

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  23. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid
    #73497
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
    Depositor
    Bruce Conklin
    Article
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Inserts
    dCas9-KRAB-P2A-mCherry
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available Since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73497-plasmid-map-sequence-id-247304

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  25. pET23b-2E2-HA-mEGFP-6xHis

    Plasmid
    #129594
    Purpose
    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    Tags
    6xHis-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 28, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129594-plasmid-map-sequence-id-261157

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  27. pNP3386 [For use in genome insertion and off-target assessment]

    Plasmid
    #247258
    Purpose
    Example plasmid for bridge recombinase mediated insertion and off-target assessment with puromycin selection — must clone in UMI
    Depositor
    Patrick Hsu
    Article
    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1
    Insert
    ISCro4 Recognition Site-UMI-Ef1a-PuroR-P2A-mCherry (Plasmid for Insertion Site Mapping [Example, Clone in Sequence + UMI])
    Expression
    Mammalian
    Available Since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    247258-plasmid-map-sequence-id-490391

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  29. pENTR4-HaloTag (w876-1)

    Plasmid
    #29644
    Depositor
    Eric Campeau
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Entry vector
    Tags
    HaloTag
    Available Since
    May 11, 2011
    Availability
    Academic Institutions and Nonprofits only
    29644-plasmid-map-sequence-id-220459

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Showing: 1051 - 1080 of 167150 results