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We narrowed to 16,118 results for: form

Showing: 1751 - 1800 of 16118 results

  6. pJM656

    Plasmid
    #53086
    Purpose
    A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control
    Depositor
    Ding Xue
    Article
    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d
    Insert
    Lactaherin (Mfge8 Mouse)
    Tags
    GFP (w/ introns) and signal sequence
    Expression
    Worm
    Mutation
    deleted native signal sequence, deleted C-termina…
    Promoter
    hsp-16.41
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    53086-plasmid-map-sequence-id-82567

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  7. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

    Plasmid
    #55202
    Purpose
    Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Article
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55202-plasmid-map-sequence-id-85589

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  10. pDest-Sumo

    Plasmid
    #106980
    Purpose
    LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag
    Depositor
    Han Choe
    Article
    Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10
    Type
    Empty backbone
    Tags
    His8-Sumo
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    106980-plasmid-map-sequence-id-204719

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  14. pSCALPs-HA-NFAT1 (4-460)-GFP

    Plasmid
    #88879
    Purpose
    Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP
    Depositor
    Silvia Monticelli
    Article
    Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi
    Insert
    HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
    Use
    Lentiviral
    Tags
    GFP fusion protein and HA tag
    Expression
    Mammalian
    Mutation
    amino acids 4-460 only; L78P & L287P *see bel…
    Promoter
    SFFV (spleen focus forming virus)
    Available Since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    88879-plasmid-map-sequence-id-177202

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  15. pQE80L-SpyCatcher-ELP-GFP

    Plasmid
    #69835
    Purpose
    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyCatcher-ELP-GFP
    Tags
    6x His Tag, GFP, and TEV Tag
    Expression
    Bacterial
    Promoter
    T5 promoter/lac operator element
    Available Since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69835-plasmid-map-sequence-id-183106

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  18. beta catenin binding mutant of alpha catenin sensor

    Plasmid
    #71711
    Purpose
    This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions
    Depositor
    Deborah Leckband
    Article
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    alpha-catenin Δβ-cat mutant FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 48- 163
    Promoter
    CMV
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71711-plasmid-map-sequence-id-142367

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  19. VBS mutant of alpha catenin sensor

    Plasmid
    #71710
    Purpose
    This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.
    Depositor
    Deborah Leckband
    Article
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    alpha-catenin ΔVBS FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 316-405 in α -catenin…
    Promoter
    CMV
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71710-plasmid-map-sequence-id-142366

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  32. pJH063

    Plasmid
    #243723
    Purpose
    Expresses a chimeric form of SUPV3L1, where the region between residue 114 and 351 is replaced by a 250-residue XTEN linker
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    SUPV3L1(1-114aa)-XTEN250-SUPV3L1(351aa-stop) (SUPV3L1 Human)
    Use
    Lentiviral
    Mutation
    The region between residue 114 and 351 of SUPV3L1…
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    243723-plasmid-map-sequence-id-488887

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  33. pJH106

    Plasmid
    #243727
    Purpose
    Expresses a chimeric form of PMPCA, where the region between residue 100 and 351 is replaced by a 250-residue XTEN linker
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    PMPCA(1-100aa)-XTEN250-PMPCA(351aa-stop) (PMPCA Human)
    Use
    Lentiviral
    Mutation
    The region between residue 100 and 351 of PMPCA i…
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    243727-plasmid-map-sequence-id-488884

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  34. pLV-SFFV-CKAP4(1-131)-WPRE-UbC-Emerald

    Plasmid
    #225955
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutant lacking the luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Evan Reid, Bart Nieuwenhuis
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Truncated CKAP4 (1-131) lacking the luminal domain
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225955-plasmid-map-sequence-id-446861

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  35. pLV-SFFV-RTN4-WPRE-UbC-Emerald

    Plasmid
    #225938
    Purpose
    Lentiviral vector plasmid expressing human reticulon 4 (RTN4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Evan Reid, Bart Nieuwenhuis
    Inserts
    RTN4 (RTN4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225938-plasmid-map-sequence-id-446791

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  36. pcs2-cDELE1(142-515)-sumo

    Plasmid
    #226107
    Purpose
    cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    DELE1 (DELE1 Human)
    Tags
    Sumo
    Expression
    Mammalian
    Mutation
    encodes only residues 142-515 of DELE1 (represent…
    Available Since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    226107-plasmid-map-sequence-id-449375

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  37. pCAG_SRGAP2A_GAP(R527L_K566A)

    Plasmid
    #222617
    Purpose
    Expresses full length SRGAP2 with R527L, K566A mt in the Rac1-specific GAP domain that abrogates the GTPase activating function of SRGAP2 without locking the GAP-domain in a form bound to Rac1-GDP.
    Depositor
    Franck Polleux
    Article
    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1
    Insert
    SRGAP2 (SRGAP2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Point mutations R527L and K566A (Rac1-specific GA…
    Available Since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222617-plasmid-map-sequence-id-448408

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  42. T40A+Q54A

    Plasmid
    #217913
    Purpose
    A plasmid encoding the hydrogel-forming protein PXP with both the T40A and Q54A coil mutations
    Depositor
    Cole DeForest
    Article
    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d
    Insert
    PXP T40A+Q54A
    Tags
    6x His (N and C term)
    Expression
    Bacterial
    Mutation
    Threonine at position 41 and 253 mutated to Alani…
    Promoter
    T5
    Available Since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    217913-plasmid-map-sequence-id-437015

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  43. pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)

    Plasmid
    #201545
    Purpose
    Expresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog B (RAD23B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks N-terminal residues 1-79
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201545-plasmid-map-sequence-id-395054

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  44. pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)

    Plasmid
    #201546
    Purpose
    Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog B (RAD23B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks residues 188-228
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201546-plasmid-map-sequence-id-395053

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  45. pCMV6-AN-DDK_WT RAD23B_delta aa 276-339 (delta XPC-binding domain)

    Plasmid
    #201547
    Purpose
    Expresses a mutant form of human RAD23B that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog B (RAD23B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks residues 276-339
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201547-plasmid-map-sequence-id-395051

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  46. pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)

    Plasmid
    #201548
    Purpose
    Expresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog B (RAD23B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks C-terminal residues 364-409
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201548-plasmid-map-sequence-id-395045

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  47. pHW539

    Plasmid
    #198811
    Purpose
    Dominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons.
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::PKA(DN)-SL2-GFP::let-858 3'UTR
    Expression
    Worm
    Mutation
    G310D mutation to abolish the binding of cAMP of …
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198811-plasmid-map-sequence-id-391700

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  48. rd

    Plasmid
    #112912
    Purpose
    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag
    Depositor
    C. James McKnight
    Article
    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20
    Insert
    dematin (DMTN Human)
    Tags
    Glutatione-S-Tranferase and precision protease si…
    Expression
    Bacterial
    Mutation
    PEST sequence near the N-terminal removed. 5′-GCG…
    Promoter
    T7
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112912-plasmid-map-sequence-id-220213

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Showing: 1751 - 1800 of 16118 results