We narrowed to 22,696 results for: Sis;

1. pGWB529

   Plasmid

   #74871

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p413TEF-SirT1

   Plasmid

   #51746

   Purpose

   overexpression of human SirT1 in S. cerevisiae, HIS3 marker

   Depositor

   Markus Ralser

   Article

   Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

   Insert

   SirT1 (SIRT1 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 RL3Sc

   Plasmid

   #109365

   Purpose

   Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA6-LRRC55-V5-Flag-His

   Plasmid

   #127819

   Purpose

   Expression of human LRRC55 in mammalian cells

   Depositor

   Jiusheng Yan

   Article

   Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

   Insert

   LRRC55 (LRRC55 Human)
   

   Use

   Tags

   V5, FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5

   Plasmid

   #52539

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attPx3 (70 bp each)
   Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. p045

   Plasmid

   #70057

   Purpose

   Co-expresses Syt1-SNARE37aa-linker complex with pDC184

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

   Inserts

   C2AB-linker-SNAP-25_C
   SNAP-25_N
   

   Use

   Tags

   No tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO HSPH1

   Plasmid

   #19453

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   HSPH1 (HSPH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRPL28(K55M) SpHIS5 (pNTI666)

   Plasmid

   #115436

   Purpose

   Expresses K55M RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K55M; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise

   Plasmid

   #129650

   Purpose

   Rieske FeS overexpression

   Depositor

   Susanne von Caemmerer

   Article

   Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

   Insert

   CDS of PetC gene encoding for the Rieske FeS subunit
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Codon modified for Golden Gate

   Promoter

   pZmUbi

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_SH3GLB1-1/1

    Plasmid

    #91346

    Purpose

    Protein expression and purification of human SH3 domain construct SH3GLB1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3GLB1-1/1 (SH3GLB1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFRTpsiCHECK

    Plasmid

    #154454

    Purpose

    Expression of renilla luciferase and firefly luciferase

    Depositor

    Markus Landthaler

    Article

    Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

    Insert

    Renilla luciferase, firefly luciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSR-puro-Mff1 shRNA

    Plasmid

    #37247

    Depositor

    Christopher Counter, David Kashatus

    Article

    Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

    Insert

    Mff1 (MFF Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pMRX-IP/Venus-mULK1

    Plasmid

    #58743

    Purpose

    Expresses Venus-mULK1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Retroviral

    Tags

    Venus

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pEPQD2KN0897

    Plasmid

    #177090

    Purpose

    MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H driven by 4x opaatB

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGWB443

    Plasmid

    #74829

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVPS13D-I-EGFP

    Plasmid

    #176464

    Purpose

    express internally GFP-tagged VPS13D in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    EYFP is internally inserted

    Promoter

    CMV

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDF-NtXNtS1R1AtR2B2

    Plasmid

    #160880

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcS-S1, rbcX, raf1, raf2, bsd2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-S1: T7, chaperones: T7

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-Arl13b2

    Plasmid

    #40871

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available Since

    Nov. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

19. dsRedm-Rab21

    Plasmid

    #83425

    Purpose

    Full-length mouse Rab21 (wildtype)

    Depositor

    Johanna Ivaska

    Article

    Pellinen et al Dev Cell. 2008 Sep;15(3):371-85. doi: 10

    Insert

    Rab21 (Rab21 Mouse)
    

    Use

    Tags

    dsRed-monomer

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FKBP_cPPVp

    Plasmid

    #118967

    Purpose

    Expresses C-terminus of plum pox virus protease (PPVp) fused to the FK-506 binding protein (FKBP)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cPPVp in fusion with FKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FRB_nPPVp

    Plasmid

    #118966

    Purpose

    Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nPPVp in fusion with FRB
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHH0103_RUSC1-1/1

    Plasmid

    #91379

    Purpose

    Protein expression and purification of human SH3 domain construct RUSC1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    RUSC1-1/1 (RUSC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-h-mmPtch1-FL-TagBFP

    Plasmid

    #120921

    Purpose

    Expresses BFP tagged Ptch1

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAGGS-Flag-hsDicer (E1564A)

    Plasmid

    #41587

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E1564A

    Promoter

    CMV

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

25. RCAS-HA-b-actin

    Plasmid

    #125834

    Purpose

    Retroviral expression of HA-tagged human beta-actin

    Depositor

    Yi Li

    Article

    Toneff et al Neoplasia. 2010 Sep;12(9):718-26.

    Insert

    Beta-actin (ACTB Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAcGP67A-rCL1-HormR-GAIN

    Plasmid

    #37847

    Depositor

    Axel Brunger

    Article

    Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

    Insert

    CL1 (Adgrl1 Rat)
    

    Use

    Baculovirus transfer vectors

    Tags

    8xHIS

    Expression

    Mutation

    HormR and GAIN domains only (aa460–849

    Promoter

    Polyhedrin

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pCDF-NtXR1AtR2B2NtS1

    Plasmid

    #160879

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcS-S1, rbcX, raf1, raf2, bsd2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rbcS-S1: pBAD, chaperones: T7

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-TO-HA-SH3BP1 (RNAi-res)

    Plasmid

    #136645

    Purpose

    Expression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

    Depositor

    Maria Balda, Karl Matter

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1 (RNAi resistant) (SH3BP1 Human)
    

    Use

    Tags

    HA (MQDLPGNDNSTAG)

    Expression

    Mammalian

    Mutation

    GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC

    Promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p3xFLAG-CMV-14-Mfn1-T562A

    Plasmid

    #191976

    Purpose

    To produce mutants mimicking dephosphorylated Mfn1

    Depositor

    Luca Scorrano

    Article

    Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

    Insert

    Mfn1 T562A (Mfn1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T562A

    Promoter

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p3xFLAG-CMV-14-Mfn1-T562D

    Plasmid

    #191977

    Purpose

    To produce mutants mimicking phosphorylated Mfn1

    Depositor

    Luca Scorrano

    Article

    Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

    Insert

    Mfn1 T562D (Mfn1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T562D

    Promoter

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge