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pTns(Ptr)

Plasmid

#175576

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTnsABC

Expression

Bacterial

Mutation

NO

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP-T-Kan

Plasmid

#44906

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP-T

Expression

Yeast

Available Since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only

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BiDi-[mp-mCherry2]mp-mEGFP

Plasmid

#182871

Purpose

Bi-directional expression vector of monomers with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

Insert

myr-palm-mCherry2, myr-palm-mEGFP

Expression

Mammalian

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-3Myc

Plasmid

#221046

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xMyc epitopes

Use

Unspecified

Promoter

read through

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pICSL50009

Plasmid

#50309

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal HA tag (6x Human influenza hemagglutinin)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAS3-5xQUAS+Δpes-10P::AI::eTsChR

Plasmid

#232612

Purpose

Expression of opsin 'eTsChR' on activation via 'QF+hGR' chimeric protein

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

eTsChR

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBT317_pET-PhiC31o-pA

Plasmid

#52491

Purpose

for preparation of PhiC31o mRNA

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PhiC31o integrase

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mCardinal

Plasmid

#179796

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mCardinal

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG

Plasmid

#179817

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSFFV_CloGFP(1-10)

Plasmid

#157994

Purpose

Lentiviral vector of constitutive expression of CloGFP(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

CloGFP(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

G32Q, F46L, K101R, S202L

Promoter

SFFV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-10FLAG

Plasmid

#181940

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xFLAG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBx-Spas-sgRNA-Kan

Plasmid

#105233

Purpose

Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescens

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCFD5-NS

Plasmid

#149546

Purpose

Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pME-BrainbowTEC

Plasmid

#82405

Purpose

Gateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HA

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Inserts

tdTomatoMYC fusion
EGFP
E2CrimsonHA

Tags

HA and MYC

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-CLIPtag

Plasmid

#179787

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

CLIP

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKate2-SpHis5

Plasmid

#44852

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKate2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKate2

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-10HA

Plasmid

#181941

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xHA

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCSCre2

Plasmid

#31308

Depositor

Gerhart Ryffel

Article

Ryffel et al Nucleic Acids Res. 2003 Apr 15. 31(8):e4

Insert

Cre recombinase (cre bacteriophage P1)

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pGST-EGFP-GPIBY55

Plasmid

#213714

Purpose

Express the affinity sorting tag GST-EGFP- GPIBY55.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGST-EGFP-GPIDAF

Plasmid

#213713

Purpose

Express the affinity sorting tag GST-EGFP- GPIDAF.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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