178,769 results
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Plasmid#211578PurposeCMV driven PARP1-eGFP expressing construct with IRES-Neomycin selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPARP1 (PARP1 Human)
TagseGFPExpressionMammalianPromoterCMVAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-CMV-Galpha-s ONE-GO
Plasmid#189731PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable sinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHCMV_COCVmut_G
Plasmid#207262PurposeMammalian expression of a mutant Cocal virus G proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCOCVmut_G
ExpressionMammalianMutationK47A/Y209A/R354A; mammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.10_Gag-MCP-P2A-eGFP
Plasmid#205525PurposeExpress HIV Gag-MCP-P2A-eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGag-MCP
ExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1346 U6-B2M sgRNA Gag-pol v2
Plasmid#201917PurposeCas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsGag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
ExpressionMammalianPromoterCAG and Human U6Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
FSW_jGCaMP8f
Plasmid#197034PurposeExpress GCaMP8f from hSyn1 promoterDepositorInsertGCaMP8f
UseLentiviralExpressionMammalianAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
v1em-Cterm-PE2max-U6-pegRNA
Plasmid#198733PurposeAAV genome encoding C-terminal PE2max and U6 expression cassetteDepositorInsertNpuC-CtermPE2max
UseAAVPromoterEFSAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (LaG17)
Plasmid#188380PurposeLentiviral vector - constitutive expression of an anti-GFP SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiGFP_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
UseLentiviralAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE3g-mNeon-WPRE-pA
Plasmid#192064PurposemNeonGreen expression with TRE3g promoterDepositorInsertmNeonGreen
UseAAVExpressionMammalianPromoterTRE3gAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CLYBL-Bxb1-LP-v2-TC
Plasmid#194327PurposeCLYBL targeting construct with the Bxb1 landing pad version 2 cassette.DepositorInsertloxP-EBFP2-attP-BleoR-lox251
UseCre/Lox and Synthetic BiologyExpressionMammalianAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-NEMOc
Plasmid#189933PurposeMammalian expression of calcium sensor with ultra-high dynamics and sensitivityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNEMOc
ExpressionMammalianPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLVX-TetOne-Puro-p21
Plasmid#171122PurposeDoxycycline inducible expression of p21DepositorAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS395_PhCMV-SB100
Plasmid#169633PurposeExpression vector encoding PhCMV driven SB100 sleeping beauty transposase (PhCMV-SB100-pA)DepositorInsertPCMV-driven SB100
ExpressionMammalianPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)
Plasmid#182287PurposeEncodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
Tagsnuclear export signal (NES)ExpressionMammalianMutationY306A/Y384F (AF) double mutant of Methanosarcina …PromoterCMV and U6Available sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GPI-mEGFP
Plasmid#182866PurposeMonomer expression vector with plasma membrane localization signalDepositorInsertGPI-mEGFP
ExpressionMammalianAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RpHLuorin2
Plasmid#180227Purposefor bacterial expression of recombinant ratiometric pHluorin2DepositorInsertRatiometric pHLuorin2
TagsHis-tag followed by thrombin cleavage siteExpressionBacterialPromoterT7Available sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX4T3-GST-Tev
Plasmid#177142PurposeBacterial vector for expression of an N-terminal GST fusion of the Tev proteaseDepositorInsertTev
TagsGSTExpressionBacterialPromoterTacAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
bact2-mKate2-P2A-TurboID-dVHH[GFP]
Plasmid#163857PurposeZebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBHDepositorInsertTurboID-VHH[EGFP]
UseZebrafish expressionTagsmKate2-P2A-TurboIDAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRU295
Plasmid#167694PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU41
Plasmid#167663PurposeENTRY vector for gRNA1 cloning under pAtU6 promoterDepositorInsertpAtU6
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONRp5-p2 SspB-mCherry-CShroom3
Plasmid#170977PurposeC-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCShroom3 (Shroom3 Mouse)
UseGateway CloningTagsSspB and mCherryMutationIsoform X2, deleted aminoacids 1 - 1388Available sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
AiP1010-pAAV-mscRE4-minBGpromoter-iCre-WPRE-hGHpA
Plasmid#163476PurposeDirect-expressing iCre AAV Virus. Alias: AiP1010 - pAAV-AiE2004m-minBG-iCre-WPRE-HGHpADepositorInsertiCre
UseAAVPromoterBeta Globin minimal promoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
OMMGFP 1-10
Plasmid#164119PurposeExpresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMMDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOMMGFP 1-10
ExpressionMammalianAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
H2B-mGreenLantern
Plasmid#164464PurposeLocalization of mGreenLantern fluorescent protein to histone 2BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmGreenLantern
TagsHuman Histone 2B (D26G and V119I mutations)ExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eMagAF-EGFP-PM
Plasmid#162247PurposeBlue-light dependent heterodimerization systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteMagAF-EGFP-CAAX
ExpressionMammalianPromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CTCF-mAC donor (Hygro)
Plasmid#140646PurposeCTCF tagging with mAID-cloverDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCTCF (CTCF Human)
UseCRISPRExpressionMammalianAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiGuide-hygro
Plasmid#160090PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance markerDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLEX307-SARS-CoV-2-3CL
Plasmid#160278PurposeAllows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralExpressionMammalianPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by