171,827 results
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Plasmid#59409PurposeExpression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]DepositorAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only
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pSelAct-KO
Plasmid#174374PurposePlasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatibleDepositorTypeEmpty backboneUseVector for 2-step knockout by homologous recombin…PromoterN/AAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA flag-Pcbp2
Plasmid#184028PurposeCodon optimized Pcbp2 clone with N-terminal flag tagDepositorAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR TDP43 WT YFP
Plasmid#27470DepositorAvailable SinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLEX304-mNeonGreen
Plasmid#162034PurposeTagged vector controlDepositorTypeEmpty backboneUseLentiviralTagsNeon GreenAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-pegRNA-HEK3_CTT_ins
Plasmid#132778PurposeS. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editingDepositorInsertHEK3_CTT_ins pegRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Prham-M.EcoRI-p15A-aadA
Plasmid#149341Purposeexpresses M.EcoRI DNA methyltransferase in E. coliDepositorInsertM.EcoRI
ExpressionBacterialPromoterrhamnoseAvailable SinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS415-GPD
Plasmid#183940PurposeControl vector that has a GPD promoter with no insertDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-SDHB-Halo7Tag
Plasmid#108973PurposeSelflabeling tag at succinate dehydrogenase complex subunit B (SDHB)DepositorInsertsuccinate dehydrogenase complex iron sulfur subunit B (SDHB Human)
TagsHalo7-tagExpressionMammalianPromoterCMVAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
G13-CASE
Plasmid#168127PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F125/D126 within GNA13Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
OmpF
Plasmid#213135PurposeExpresses outer membrane protein FDepositorAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_hACE2_PURO
Plasmid#155295PurposeLentiviral vector to generate hACE2 stable expressing cell lineDepositorAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-HA-MAVS-M142A(KaganF61)
Plasmid#51999PurposeExpresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"DepositorInsertMAVS
TagsHAExpressionMammalianMutationMet 142 to AlaAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE hygro MEN1 WT
Plasmid#11024DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-PuroR-2A-MLH1-SB
Plasmid#228871PurposePlasmid for MLH1-SB overexpressionDepositorInsertPuroR-2A-MLH1-SB
UseCRISPRAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6f.WPRE.SV40
Plasmid#100837PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterSynapsinAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF2703
Plasmid#144151PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETZZtevPP7CP(C68A,C73A)
Plasmid#27548DepositorInsertPseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
TagsTEV protease cleavage site, six histidine tag, an…ExpressionBacterialMutationchanged two cysteine to alanine (C68A,C73A) to in…Available SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-GFP
Plasmid#187840PurposeConstitutive GFP-expression vector for Fusobacterium nucleatumDepositorInsertsfGFP
ExpressionBacterialAvailable SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL-3F-BirA
Plasmid#220147PurposeExpresses VHL-BirA for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationcodon optimizedPromoterEF-1aAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-GAL4-ERT2-VP16
Plasmid#82588PurposeMiddle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expressionDepositorInsertGAL4-ERT2-VP16
PromoterNoneAvailable SinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-cMyc
Plasmid#46970Purposelentiviral expression of c-MycDepositorAvailable SinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
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non-targeting control gRNA (BRDN0001148862)
Plasmid#80263Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pB-TREtight-EGFP
Plasmid#182967PurposeFor making cell lines for titering pRV∆L-5tTA virusDepositorInsertEGFP
UseUnspecifiedAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-tRNA2.0
Plasmid#158394PurposeGolden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Po-Mt Short P2A
Plasmid#164116PurposeDetect the short Peroxisomes-mitochondria contactDepositorInsertSplit GFP Po-Mt Short P2A
ExpressionMammalianAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCX-DI-2A
Plasmid#74671PurposeMammalian expression of DI-2A (hCD73/F2A/hCD39)DepositorAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-CalfluxVTN
Plasmid#83926PurposeBioluminescent Reporter of Intracellular CalciumDepositorInsertCalfluxVTN
ExpressionBacterialMutationNonePromoterT7Available SinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only