19,755 results

1. SepRS(2)/pSertRNA(B4)/EF-Sep

   Plasmid

   #173897

   Purpose

   The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

   Depositor

   Jason W Chin

   Article

   Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

   Inserts

   SepRS(2)
   pSertRNA
   EF-Sep
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCVFLAG-ZFP423

   Plasmid

   #24764

   Depositor

   Bruce Spiegelman

   Article

   Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

   Insert

   Zinc Finger Protein 423 (Zfp423 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 14, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pDEST-eGFP-C1-VPS35

   Plasmid

   #163622

   Purpose

   human VPS35 cDNA C-terminally tagged with eGFP

   Depositor

   Josef Kittler

   Article

   Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

   Insert

   human VPS35 (VPS35 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PDX1-2A-eGFP

   Plasmid

   #66964

   Purpose

   PDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locus

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

   Insert

   pancreatic and duodenal homeobox 1 (PDX1 Human)
   

   Use

   Unspecified

   Tags

   2A-eGFP

   Available Since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIMAY

   Plasmid

   #68939

   Purpose

   E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

   Depositor

   Tim Foster

   Article

   Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

   Type

   Empty backbone

   Use

   Staphylococcal allelic exchange vector

   Available Since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-HypaCas9

   Plasmid

   #108302

   Purpose

   pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti.pFOXA2.GFP

   Plasmid

   #59404

   Purpose

   expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

   Depositor

   Kai-Christian Sonntag

   Article

   Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

   Insert

   400 bp FOXA2 promoter, eGFP (FOXA2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   pFOXA2

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGHYB

   Plasmid

   #87447

   Purpose

   Pichia pastoris expression plasmid

   Depositor

   Sheng Yang

   Article

   Yang et al Yeast. 2014 Apr;31(4):115-25.

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   GAP

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMaCTag-P05

   Plasmid

   #120016

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   eGFP
   

   Use

   Pcr template

   Tags

   eGFP

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW-Cas9

    Plasmid

    #50661

    Purpose

    Doxycycline-inducible lentiviral expression of SpCas9

    Depositor

    Eric Lander, David Sabatini

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    Tet ON

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-Nck1

    Plasmid

    #45903

    Depositor

    Louise Larose

    Article

    Latreille et al J Biol Chem. 2006 Sep 8;281(36):26633-44

    Insert

    Nck-1 (NCK1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-TRAIL-R2

    Plasmid

    #61383

    Purpose

    Expresses TRAIL receptor 2

    Depositor

    Francis Chan

    Article

    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

    Insert

    TRAIL receptor 2 (TNFRSF10B Human)
    

    Tags

    none

    Expression

    Mammalian

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMA7-SacB

    Plasmid

    #79968

    Purpose

    TM-MAGE strain

    Depositor

    Morten Sommer

    Article

    Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

    Inserts

    Lambda Red recombinase beta subunit
    DNA adenine methylase
    Levansucrase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYES2-Ub-G76V-GFP

    Plasmid

    #11954

    Depositor

    Nico Dantuma

    Article

    Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

    Insert

    Ub-G76V-GFP
    

    Expression

    Yeast

    Mutation

    Ubiquitin fused to N-terminus of GFP. Glycine 76 …

    Available Since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAD-LyseR (in lacZ deficient strain)

    Plasmid

    #99245

    Purpose

    For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

    Depositor

    Jeff Hasty

    Article

    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

    Insert

    phage lambda gene R (R phage lambda)
    

    Expression

    Bacterial

    Promoter

    beta-lactamase promoter

    Available Since

    Oct. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ220 (AsCpf1)

    Plasmid

    #86208

    Purpose

    AsCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    AsCpf1
    

    Use

    Gateway compatible cpf1 vectors

    Tags

    5' and 3' NLS

    Expression

    Plant

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSRK2721

    Plasmid

    #78572

    Purpose

    Gateway destination vector for production of MBP-3C site-His6-TEV site-passenger protein fusions

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

    Insert

    Gateway cloning cassette
    

    Expression

    Bacterial

    Available Since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL1142 (pSPIN, pBBR1 backbone)

    Plasmid

    #160730

    Purpose

    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ptc1-YFP

    Plasmid

    #58456

    Purpose

    expression of mouse Ptc1-YFP

    Depositor

    Matthew Scott

    Article

    Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

    Insert

    Ptc1 (Ptch1 Mouse)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pKHR4

    Plasmid

    #74592

    Purpose

    an ampR plasmid built from pBluescript: the multiple cloning site (mcs) flanked by inverted head-to-head oriented I-SceI sites. Genbank accession number is KU144822.

    Depositor

    David Grunwald

    Article

    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

    Type

    Empty backbone

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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