2,225 results

1. NF-155 pcDNA3

   Plasmid

   #197290

   Purpose

   Plasmid expressing an antigen targeting the Plasmid expressing an antigen targeting Neurofascin-155

   Depositor

   Peter Brophy

   Article

   Collinson et al Glia. 1998 May;23(1):11-23

   Insert

   Pan-Neurofascin (extracellular) (Nfasc Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. tdTomato-Lysosomes-20

   Plasmid

   #58109

   Purpose

   Localization: Lysosome Membrane, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Insert

   LAMP1
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pAcGP67A-rCL1-HormR-GAIN

   Plasmid

   #37847

   Depositor

   Axel Brunger

   Article

   Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

   Insert

   CL1 (Adgrl1 Rat)
   

   Use

   Baculovirus transfer vectors

   Tags

   8xHIS

   Expression

   Mutation

   HormR and GAIN domains only (aa460–849

   Promoter

   Polyhedrin

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

4. mVenus-RnBarr2

   Plasmid

   #137799

   Purpose

   Visualization of beta-arrestin2

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat beta-arrestin2 (Arrb2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenLox_U6 Nlgn2

   Plasmid

   #59358

   Purpose

   Lentiviral expression vector: Neuroligin 2 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

   Depositor

   Peter Scheiffele

   Article

   Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

   Inserts

   Neuroligin 2 shRNA (rat and mouse) (Nlgn2, Nlgn2 Rat, Mouse)
   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and mouse U6

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. LUC45 (L/Sec/mutP)

   Plasmid

   #112148

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U) and SE…

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOC1 Gria1-GFP-FRB KI

   Plasmid

   #183428

   Purpose

   CreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and GFP-FRB donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOC2 Dlg4-Halo-FKBP KI

   Plasmid

   #183435

   Purpose

   CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and Halo-FKBP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCI.neo-rPAM1-M314I

   Plasmid

   #145802

   Purpose

   expresses catalytically inactive rat PAM-1 in mammalian cells

   Depositor

   Betty Eipper, Richard Mains

   Article

   Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

   Insert

   rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed methionine 314 to isoleucine to inactivat…

   Promoter

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX-SV40-Galpha-i1 ONE-GO

    Plasmid

    #204328

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    SV40 Galpha-i1 ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. Dronpa-GluR2

    Plasmid

    #62735

    Purpose

    Excitation:503, Emission:518

    Depositor

    Michael Davidson

    Insert

    GluR2
    

    Use

    Tags

    Dronpa

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pEGFP-Sec22b-P33-shR

    Plasmid

    #208359

    Purpose

    Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutant

    Depositor

    Paula Nunes-Hasler

    Article

    Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

    Insert

    Sec22b (Sec22b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    four silent mutations (shRNA resistance), 33 aa p…

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEF Doc2alpha WT GFP

    Plasmid

    #128819

    Purpose

    To visualize Doc2alpha WT in PC12 cells

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA 3.1 AnxA6

    Plasmid

    #20407

    Depositor

    Carlos Enrich

    Article

    Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

    Insert

    AnxA6 (Anxa6 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

15. RSV CREB

    Plasmid

    #22394

    Depositor

    Marc Montminy

    Article

    Gonzalez et al Cell. 1989 Nov 17. 59(4):675-80.

    Insert

    CREB (Creb1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pR2Q347y

    Plasmid

    #112680

    Purpose

    functional, fluorescent, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    Glutamate receptor subunit (Gria2 Rat)
    

    Use

    Tags

    fluorescent protein

    Expression

    Mammalian

    Mutation

    tagged with fluorescent protein

    Promoter

    cmV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCB6HindIII-rat FcRn

    Plasmid

    #121671

    Purpose

    Expresses rat neonatal Fc receptor in mammalian cells

    Depositor

    Pamela Bjorkman

    Article

    Tesar et al Traffic. 2006 Sep;7(9):1127-42.

    Insert

    rat neonatal Fc receptor (Fcgrt Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3 Flag p38 gamma (agf)

    Plasmid

    #20354

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 gamma(agf) (Mapk12 Rat)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Replace Thr 183 and Tyr 185 with Ala and Phe resp…

    Promoter

    Available since

    April 29, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pCS2+-H2B-pr-mEosFP

    Plasmid

    #141101

    Purpose

    H2B fused to pr-mEosFP for chromatin visualization and primed conversion

    Depositor

    Periklis Pantazis

    Article

    Welling et al Bio Protoc. 2020 Jun 5;10(11):e3645. doi

    Insert

    Histone 2B
    

    Use

    Tags

    pr-mEosFP

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5FRT/TO-p97wt-mycStrep

    Plasmid

    #31837

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Use

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

21. pSIL-eGFP-shACTN2

    Plasmid

    #52677

    Purpose

    shRNA against α-actinin-2 with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Insert

    ACTN2 (Actn2 Rat)
    

    Use

    RNAi

    Tags

    CMV-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-hPGK-mCherry-SynaptoZip

    Plasmid

    #177317

    Purpose

    Lentiviral expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by hPGK promoter

    Depositor

    Antonio Malgaroli

    Article

    Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

    Insert

    mCherry-SynaptoZip (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    Myc and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Stx3-pmCitrine

    Plasmid

    #92421

    Purpose

    Expression of syntaxin-3 C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx3 (Stx3 Rat)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pORANGE Cplx2-GFP KI

    Plasmid

    #131476

    Purpose

    Endogenous tagging of Complexin2: C-terminal (amino acid position: L128)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cplx2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCI-neo-myc-LC3(G120A)-HA

    Plasmid

    #45245

    Depositor

    Tamotsu Yoshimori

    Article

    Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Glycine at position 120 mutated to Alanine

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

26. mTFP1-CathespinB-6

    Plasmid

    #55467

    Purpose

    Localization: Lysosomes, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    CathespinB
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

27. AAV Synapsin Intron H2B Gcamp 6f wpre Pzac2.1

    Plasmid

    #74144

    Purpose

    Calcium sensor

    Depositor

    Loren Looger

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    H2B

    Expression

    Mutation

    Promoter

    synapsin

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pXL-CAG-3XAP-NLG1

    Plasmid

    #43923

    Depositor

    Alice Ting

    Article

    Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

    Insert

    3XAP-NLG1
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta Actin

    Available since

    April 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBactin-Nedd1-mNeonGreen

    Plasmid

    #196864

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mNeonGreen (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBactin-td-mCherry-Cdk5rap2

    Plasmid

    #196859

    Purpose

    Expression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    td-mCherry-Cdk5rap2 (Cdk5rap2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge