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We narrowed to 6,995 results for: tet

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Showing: 1801 - 1850 of 6995 results

  1. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Article
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  4. pTET-IUPi

    Plasmid
    #122019
    Purpose
    Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.
    Depositor
    Gregory Stephanopoulos
    Article
    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p
    Inserts
    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    pTET
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    122019-plasmid-map-sequence-id-238972

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  5. pFGL1252_TetOFF(Hyg)

    Plasmid
    #118992
    Purpose
    TetOFF (promoter-less) cassette with Hygromycin selection
    Depositor
    Naweed Naqvi
    Article
    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581
    Inserts
    tTA+Tnos
    Hygromycin cassette
    Ttub+PtetO
    Use
    Fungal expression (in magnaporthe oryzae)
    Mutation
    Codon optimized for Magnaporthe oryzae
    Promoter
    Aspergillus nidulans TrpC promoter, Ustilago mayd…
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    118992-plasmid-map-sequence-id-232118

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  7. pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

    Plasmid
    #117005
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    117005-plasmid-map-sequence-id-227039

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  10. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid
    #218629
    Purpose
    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
    Depositor
    Valentin Cracan
    Article
    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038
    Insert
    mitoEcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and MTS
    Expression
    Mammalian
    Available Since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218629-plasmid-map-sequence-id-432199

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  11. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid
    #129025
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129025-plasmid-map-sequence-id-454365

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  12. pUS212

    Plasmid
    #84580
    Purpose
    E.coli cloning and expression vector. Has cloning sites downstream of Tet promoter.
    Depositor
    Nicholas Coleman, Mark Somerville
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Nov. 21, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    84580-plasmid-map-sequence-id-166638

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  16. SIN40C.TRE.dTomato.miR30n.PGK.sfGFP.P2A.Tet3G

    Plasmid
    #169282
    Purpose
    Doxycycline-inducible overexpression of miRNA or shRNA with dTomato as a reporter fluorescent protein
    Depositor
    Dirk Heckl
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169282-plasmid-map-sequence-id-337922

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  22. pUltraI-Tet3.0[TAA]

    Plasmid
    #164580
    Purpose
    Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons
    Depositor
    Ryan Mehl
    Article
    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2
    Inserts
    Mb Tet3.0RS (R2-3)
    Mm tRNA[TAA]
    Expression
    Bacterial
    Mutation
    L170G, N311G, C313A
    Promoter
    ProK and TacI
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    164580-plasmid-map-sequence-id-392917

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  40. pLV-UbC-Tet1v4-dCas9

    Plasmid
    #232180
    Purpose
    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells
    Depositor
    Charles Gersbach
    Article
    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi
    Insert
    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    hUbC
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232180-plasmid-map-sequence-id-462961

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  42. pENTR-THT III

    Plasmid
    #55791
    Purpose
    GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistance
    Depositor
    Stephan Geley
    Article
    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:
    Type
    Empty backbone
    Use
    Gateway Cloning and RNAi
    Expression
    Mammalian
    Promoter
    modified H1 RNA gene
    Available Since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    55791-plasmid-map-sequence-id-89573

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  43. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

    Plasmid
    #184398
    Purpose
    Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    Renilla luciferase beta-globin-PTC39
    Use
    CRISPR; Donor plasmid for hdr
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    PTC in beta-globin at amino acid position 39
    Promoter
    Tet-On 3G
    Available Since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184398-plasmid-map-sequence-id-362369

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  45. pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG

    Plasmid
    #129758
    Purpose
    Dox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.
    Insert
    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    changed Glutamic acid 36 to Alanine and Leucine 3…
    Promoter
    TRE3Gs and Tet inducible promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129758-plasmid-map-sequence-id-257095

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  50. Tet-O-FUW-OVOL2-3xFLAG-PURO

    Plasmid
    #251740
    Purpose
    Overexpression construct expressing OVOL2-3xFLAG-PURO and ORF-BC
    Depositor
    Andrea Califano
    Insert
    OVOL2-3xFLAG (OVOL2 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251740-plasmid-map-sequence-id-501484

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Showing: 1801 - 1850 of 6995 results