We narrowed to 16,540 results for: puromycin

1. pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)

   Plasmid

   #214879

   Purpose

   Lentiviral vector encoding RfxCas13d targeting SURF2 guide array

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   hU6-crSURF2-EFS-PuroR-WPRE
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-p300

   Plasmid

   #191764

   Purpose

   Expression of EGFP fused to core p300 histone acetyltransferase

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   FRB-EGFP-p300 core (EP300 Human)
   

   Tags

   EGFP (N-terminal of p300 core) and FRB (N-termina…

   Expression

   Bacterial and Mammalian

   Promoter

   EF1alpha

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-N-Puro-mStayGold-LMNB1

   Plasmid

   #227329

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Puro-2A-mStayGold (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. paGFP-Omp25

   Plasmid

   #69598

   Purpose

   expresses GFP-labeled mitochondrial outer membrane protein 25

   Depositor

   David Sabatini

   Article

   Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

   Insert

   paGFP-Omp25 (Synj2bp Rat)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   photoactivatable GFP fused to rat Omp25 C-terminus

   Promoter

   CMV

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDB_054

   Plasmid

   #216096

   Purpose

   All-in-one, knockout, delivers Cas12a & gRNA

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas12a [EnAs]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TPC1-mCherry

   Plasmid

   #135182

   Purpose

   Expresses TPC1 with C-terminus mCherry tag in mammalian cells

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC1 (Tpcn1 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScalps_Puro_mCebpa

   Plasmid

   #79551

   Purpose

   Expression of mouse Cebpa

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Cebp alpha (Cebpa Mouse)
   

   Use

   Lentiviral

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMKO.1 puro p53 shRNA 1

   Plasmid

   #10671

   Depositor

   William Hahn

   Article

   Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

   Insert

   p53 shRNA (TP53 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSH-EFIRES-P-Seipin-miniIAA7-mCherry

   Plasmid

   #129720

   Purpose

   AAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged Seipin

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   Seipin (BSCL2 Human)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   miniIAA7-mCherry

   Expression

   Mammalian

   Mutation

   silent mutations

   Promoter

   EF1a

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2848

    Plasmid

    #144249

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF21 (TCF21 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX317-VRK1

    Plasmid

    #199643

    Purpose

    Expresses VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF0169

    Plasmid

    #141540

    Purpose

    Lentiviral vector for overexpressing the SIX3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SIX3 (SIX3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF2474

    Plasmid

    #144029

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ONECUT3 (ONECUT3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. GUT BuGZ FL

    Plasmid

    #84024

    Purpose

    Expression of GFP tagged BuGZ in human cells

    Depositor

    Patrick Paddison

    Article

    Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

    Insert

    BuGZ (ZNF207 Human)
    

    Use

    Lentiviral

    Tags

    TurboGFP

    Expression

    Mammalian

    Mutation

    Q394R

    Promoter

    UCOE / SFFV

    Available Since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-3FLAG-RAB11A

    Plasmid

    #176489

    Purpose

    For lentiviral transduction of 3FLAG-RAB11A

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. RNASEL gRNA (BRDN0001149212)

    Plasmid

    #77051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. RNASEL gRNA (BRDN0001149358)

    Plasmid

    #77052

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiRNAGuide_003

    Plasmid

    #192505

    Purpose

    For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42333

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF0098

    Plasmid

    #142460

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MSGN1 (MSGN1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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