We narrowed to 22,697 results for: Sis;

1. pHH0103_SLA2-1/1

   Plasmid

   #91450

   Purpose

   Protein expression and purification of human SH3 domain construct SLA2-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SLA2-1/1 (SLA2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_NCK2-2/3

   Plasmid

   #91362

   Purpose

   Protein expression and purification of human SH3 domain construct NCK2-2/3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   NCK2-2/3 (NCK2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-HA-RIPK3 S199A

   Plasmid

   #78806

   Purpose

   To overexpress RIPK3 S199A in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK3 (RIPK3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S199A

   Promoter

   CMV

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti_CRISPRi_sgNeg2

   Plasmid

   #166999

   Purpose

   Lentiviral expression of negative control sgRNA for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   Negative control sgRNA 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBGN155-MXMT

   Plasmid

   #79817

   Purpose

   GFP(S65T)-based BiFC vector for plants (Positive control: MXMT)

   Depositor

   Yutaka Kodama, Masamitsu Wada

   Article

   Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

   Insert

   CaMXMT1
   

   Use

   Reporter plasmid

   Tags

   GN155/GFP (1–155)

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter and CaMV35S

   Available Since

   Aug. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. px330-RPL26 sgRNA2

   Plasmid

   #134642

   Purpose

   contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   RPL26 sgRNA2 (RPL26 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. px330-RPL26 sgRNA1

   Plasmid

   #134641

   Purpose

   contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   RPL26 sgRNA2 (RPL26 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-hygro-hPGAP3-3HA

   Plasmid

   #50373

   Purpose

   Express C-terminally triple HA tagged human PGAP3 in mammmalian cells

   Depositor

   Reika Watanabe

   Article

   Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

   Insert

   PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Promoter

   SR alpha

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pDGE660

   Plasmid

   #153258

   Purpose

   M4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-HA-mIFITM3-K83,88,104A

    Plasmid

    #58405

    Purpose

    Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lysines 83, 88, and 104 to Alanine

    Promoter

    CMV IE

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-HA-mIFITM3-K83A

    Plasmid

    #58401

    Purpose

    Expresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lysine 83 to Alanine

    Promoter

    CMV IE

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-HA-mIFITM3-K24A

    Plasmid

    #58400

    Purpose

    Expresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lysine 24 to Alanine

    Promoter

    CMV IE

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pQUAST>stop>shibire (ts1)

    Plasmid

    #24363

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    Shibire (ts1) (shi Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HA-RIPK3 K55R

    Plasmid

    #78807

    Purpose

    To overexpress RIPK3 K55R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K55R

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTrichoGate-13

    Plasmid

    #160713

    Purpose

    For C-terminal tags with Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA) bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMHBAD14

    Plasmid

    #42304

    Depositor

    Hideo Iwai

    Article

    Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

    Insert

    NpuDnaE C-intein
    

    Use

    Tags

    GB1-H6

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet

    Plasmid

    #83286

    Purpose

    untargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    mTurquoise2-FHA2-AURKB substrate-YPet
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGFP-Ub-VPS13D_dC

    Plasmid

    #176466

    Purpose

    express VPS13D lacking C-terminal 407 aa in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    C-terminal 407 aa is deleted.

    Promoter

    CMV

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEYFP-N1-hRIP3-FL V460P

    Plasmid

    #61379

    Purpose

    Expresses Human full length RIPK3 with V460P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP3 with V460Pmutation (RIPK3 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed V460 to P

    Promoter

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pEYFP-N1-hRIP3-FL V458P

    Plasmid

    #61377

    Purpose

    Expresses Human full length RIPK3 with V458P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP3 with V458Pmutation (RIPK3 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed V458 to P

    Promoter

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

21. kank2_pJet1.2

    Plasmid

    #121980

    Purpose

    for generating kank2 riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

    Insert

    kank2 (LOC571549 Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EGFP-bSV1_1-1009

    Plasmid

    #129471

    Purpose

    Mammalian expression of the N-terminus + central domain of bovine SV1

    Depositor

    Elizabeth Luna

    Article

    Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

    Insert

    Supervillin (SVIL Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains amino acids 1-1009

    Promoter

    CMV

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJK526

    Plasmid

    #155387

    Purpose

    PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA4 in dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)

    Plasmid

    #61939

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

25. UKNP4.3.1

    Plasmid

    #98269

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. UKNP3.2.2

    Plasmid

    #98247

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHH0103_NCK1-1/3

    Plasmid

    #91472

    Purpose

    Protein expression and purification of human SH3 domain construct NCK1-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCK1-1/3 (NCK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRSET FLIPglu-600uDelta11 Ares-Aphrodite

    Plasmid

    #13571

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta11 Aphrodite
    

    Use

    Tags

    Aphrodite and Ares

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA5/FRT/TO DNAJB7

    Plasmid

    #19476

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB7 (DNAJB7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEG302 22aa SunTag NtDRMcd SUP

    Plasmid

    #115489

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge