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pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Plasmid

#92392

Purpose

produces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

Human Synapsin

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-NEMOs

Plasmid

#189931

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOs

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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h-gamma-ENaC Myc

Plasmid

#83428

Purpose

full length human gamma ENaC with myc tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2008 May;294

Insert

human gamma ENaC (SCNN1G Human)

Tags

Myc

Expression

Mammalian

Mutation

P502A

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSmac-GFP

Plasmid

#40881

Depositor

Douglas Green

Article

Tait et al Dev Cell. 2010 May 18;18(5):802-13.

Insert

Smac-GFP (DIABLO Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pLJM60 FLAG NUFIP1 W40A

Plasmid

#110951

Purpose

expresses mammalian FLAG-NUFIP1 W40A

Depositor

David Sabatini

Article

Wyant et al Science. 2018 May 18;360(6390):751-758.

Insert

NUFIP1 W40A (NUFIP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed Tryptophan 40 to alanine

Promoter

CMV

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFYF1548 EMX1

Plasmid

#47508

Purpose

human gRNA expression vector targeting EMX1

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EMX1 (EMX1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pBOB-CARMIL2 BH domain-GFP

Plasmid

#118736

Purpose

To express CARMIL2 BH tagged with GFP on C-terminus

Depositor

John Cooper

Article

Lanier et al J Biol Chem. 2016 Jan 15;291(3):1076-91.

Insert

CARMIL2 BH domain (CARMIL2 Human)

Use

Lentiviral

Tags

GFP

Promoter

CMV

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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Green Pegassos

Plasmid

#163114

Purpose

Expresses biosensors for pyruvate in mammalian cells

Depositor

Takashi Tsuboi

Article

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Pegassos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-SEP GluR2(R)

Plasmid

#24001

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

GluR2 (Gria2 Rat)

Tags

GluR1 signal peptide (aa1-22) and SEP (supereclip…

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pMK286 (mAID-NeoR)

Plasmid

#72824

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Neo

Use

CRISPR

Tags

mAID

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA11-mRuby3

Plasmid

#184894

Purpose

Stable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA11 (ANXA11 Human)

Tags

mRuby3

Expression

Mammalian

Mutation

R230C relative to NP_001148.1

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-AGG-SpCas9

Plasmid

#214603

Purpose

encode SpCas9; in vitro transcribed modified RNA vector

Depositor

Yuxuan Guo

Article

Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

Insert

SpCas9

Use

CRISPR and Unspecified

Promoter

T7

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

Plasmid

#223160

Purpose

Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

dGFP-P2A-NanoLuc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MEKK1 WT

Plasmid

#12181

Depositor

Gary Johnson

Article

Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

Insert

MEKK1 (Map3k1 Mouse)

Tags

His and Xpress

Expression

Mammalian

Mutation

V1467L

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-Pc-DsRed-Express2

Plasmid

#111260

Purpose

mini-Tn7 vector for insertion of DsRed-Express2 at bacterial attTn7 site

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

DsRed-Express2

Expression

Bacterial

Promoter

Pc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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FLHKI-pGFPN3

Plasmid

#21917

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Full length human HKI in pGFP-N3 (HK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is full length human HKI in pGFP-N3

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pETM33_YES1_SH3

Plasmid

#178492

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

YES1_SH3 (YES1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6/UbC/mSlc7a1

Plasmid

#17224

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

Slc7a1 (Slc7a1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-NEMOb

Plasmid

#189934

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOb

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT-EF-Pdgfralpha-EGFPN

Plasmid

#66787

Purpose

Expression of murine PDGFRalpha tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Article

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Insert

PDGFRalpha (Pdgfra Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3 NSE promoter

Plasmid

#11606

Depositor

Rosalyn Adam

Article

Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

Insert

NSE promoter (ENO2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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Cas9-2A-Cre

Plasmid

#68468

Purpose

co-expressing Cas9 and Cre recombinase

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

Cas9 and Cre recombinase

Use

CRISPR

Tags

P2A-Cre-Myc

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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FU-tet-o-hc-myc

Plasmid

#19775

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

c-myc (MYC Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-GR11

Plasmid

#89105

Purpose

mammalian expression of full-length human glucocorticoid receptor in pCMV5

Depositor

Elizabeth Wilson

Article

Kupfer et al J Biol Chem. 1993 Aug 15;268(23):17519-2

Insert

human glucocorticoid receptor (NR3C1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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AAV sgRNA Expression Plasmid

Plasmid

#174540

Purpose

Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

Depositor

Ormond MacDougald

Article

Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

Type

Empty backbone

Use

AAV

Tags

None

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pTiGc

Plasmid

#78314

Purpose

Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).

Depositor

Samantha Sampson

Article

Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/

Inserts

TurboFP635
GFP

Use

Synthetic Biology; Mycobacterial replicating vect…

Expression

Bacterial

Promoter

hsp60 and hsp60(ribo)

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
CMV-mEGFP(A206K)-paCaMKII(K42M)

Plasmid

#165440

Purpose

Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(K42M)

Tags

mEGFP(A206K)

Expression

Mammalian

Mutation

K42M: Kinase dead mutation

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-eCas9-sgNT

Plasmid

#140238

Purpose

Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

non-targeting sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pmiCas9

Plasmid

#162758

Purpose

express miCas9

Depositor

Eugene Chen

Article

Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

Insert

miCas9

Use

CRISPR

Tags

FLAG and NLS

Expression

Mammalian

Mutation

Contains Brex27 sequence range is 4958-5065

Promoter

CMV

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor ORF2

Plasmid

#200382

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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