We narrowed to 9,109 results for: control

1. pSTAP-seq_fly-zfh1_spikeIn-1

   Plasmid

   #86387

   Purpose

   Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter
   

   Use

   Stap-seq spike in vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAM-PAT-ProCDKA;1::CDKA;1:YFP

   Plasmid

   #80685

   Purpose

   binary Expression vector for plant expression

   Depositor

   Arp Schnittger, Nico Dissmeyer

   Article

   Nowack et al Nature. 2007 May 17;447(7142):312-5. Epu

   Insert

   CDKA;1 (CDC2 Mustard Weed)
   

   Use

   Tags

   YFP

   Expression

   Plant

   Mutation

   Promoter

   ProCDKA;1

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSTAP-seq_fly-zfh1_spikeIn-2

   Plasmid

   #86388

   Purpose

   Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer & D. pseudoobscura CG32369 core promoter
   

   Use

   Stap-seq spike in vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Banshee-ShCit-A

   Plasmid

   #85216

   Purpose

   Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

   Depositor

   Alex Minella

   Article

   Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

   Insert

   shCit-A
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Banshee-ShCit-B

   Plasmid

   #85217

   Purpose

   Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

   Depositor

   Alex Minella

   Article

   Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

   Insert

   shCit-B
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVH009

   Plasmid

   #80399

   Purpose

   Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.

   Depositor

   Richard Murray

   Article

   Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

   Inserts

   3xFLAG-Venus YFP-AAV
   CusR (cusR )
   AraC (araC )
   

   Use

   Synthetic Biology

   Tags

   C-terminal LZx domain and N-terminal 3xFLAG tag, …

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. C0080 (araC)_CD

   Plasmid

   #66029

   Purpose

   MoClo Basic Part: CDS - Controller protein, araC repressor/activator (activates pBAD, I13453) [C:C0080:D]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Transcription factor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. miR-451 Trap

   Plasmid

   #61487

   Purpose

   to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter

   Depositor

   Curt Civin

   Article

   Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

   Insert

   miR-451 Trap
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   bidirectional phosphoglycerate kinase promoter (b…

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

9. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

   Plasmid

   #68356

   Purpose

   -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter

   Depositor

   Jannik Elverløv-Jakobsen

   Inserts

   4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
   FlpO recombinase
   

   Use

   CRISPR

   Tags

   mTurquoise2 (BFP)

   Expression

   Mammalian

   Mutation

   Promoter

   Synthetic Probasin ARRx2 and U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. RFN_EGFP_84

    Plasmid

    #60076

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

11. RFN_EGFP_83

    Plasmid

    #60075

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

12. RFN_EGFP_82

    Plasmid

    #60074

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

13. RFN_EGFP_80

    Plasmid

    #60072

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pHCMM3

    Plasmid

    #167454

    Purpose

    A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PSEC63_yeGFP_cePUP-2_URA3_TADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PSEC63

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBIG2abc_hsHAUScomplex_FL

    Plasmid

    #202203

    Purpose

    Baculoviral transfer vector to co-express 8 subunits of human augmin complex

    Depositor

    Thomas Surrey

    Article

    Ustinova et al J Cell Sci. 2023 Jun 15;136(12):jcs26109

    Inserts

    HAUS1 (HAUS1 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS3 (HAUS3 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS2 (HAUS2 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS6 (HAUS6 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS7 (HAUS7 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS4 (HAUS4 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS5 (HAUS5 codon-optimized form for expression in Trichoplusia ni cells, Human)
    HAUS8 (HAUS8 codon-optimized form for expression in Trichoplusia ni cells, Human)
    

    Use

    Tags

    -mGFP-TEV-TwinStrep and His-tag (6x)

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-ExRai-AMPKAR(T/A)

    Plasmid

    #192447

    Purpose

    Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR(T/A)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Thr 6 in AMPK substrate mutated to Ala.

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPolI_SARS-CoV-2_NLuc

    Plasmid

    #183953

    Purpose

    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

    Depositor

    AJ te Velthuis

    Article

    Vial et al bioRxiv 2021.06.28.450211

    Insert

    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mammalian Pol I

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAD-KPS12-Pman

    Plasmid

    #133832

    Purpose

    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    mKate(KPS12)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pman

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. SF-U7snRNA-CypA-E4

    Plasmid

    #190695

    Purpose

    Control vector expressing a U7-based antisense oligonucleotide targeting CypA exon 4

    Depositor

    Pim Pijnappel

    Article

    van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

    Insert

    U7 snRNA antisense CypA Exon 4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U7 promoter

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmVenus(L68V)-mTurquoise2

    Plasmid

    #60493

    Purpose

    Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    SYFP2-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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