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Showing: 1481 - 1520 of 2028 results

  4. pMS1251

    Plasmid
    #80163
    Purpose
    pET-Duet for bacterial expression of Ald-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme
    Depositor
    Marcelo Sousa
    Article
    Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/
    Insert
    HisSUMO-Ald-4xNuG2-Ald
    Use
    Tags
    HisSUMO
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    80163-plasmid-map-sequence-id-150737

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  6. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid
    #227275
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227275-plasmid-map-sequence-id-452039

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  7. pBIG1a_StrepII-Hook3[451-718]_FTS_FHIP1B

    Plasmid
    #222300
    Purpose
    Co-expresses truncated human FHF (FTS, StrepII-HOOK3[451-718] and FHIP1B) in a pBIG1a vector
    Depositor
    Andrew Carter
    Inserts
    HOOK3[451-718] (HOOK3 Human)
    FTS (AKTIP Human)
    FHIP1B (FHIP1B Human)
    Use
    Tags
    StrepII-Psc
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222300-plasmid-map-sequence-id-443587

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  8. pBIG1a_StrepII-Hook3[571-718]_FTS_FHIP1B

    Plasmid
    #222301
    Purpose
    Co-expresses truncated human FHF (FTS, StrepII-HOOK3[571-718] and FHIP1B) in a pBIG1a vector
    Depositor
    Andrew Carter
    Inserts
    HOOK3[571-718] (HOOK3 Human)
    FTS (AKTIP Human)
    FHIP1B (FHIP1B Human)
    Use
    Tags
    StrepII-Psc
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222301-plasmid-map-sequence-id-443588

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  10. pAAV-sCAG-GluClα.CreON

    Plasmid
    #196995
    Purpose
    Cre recombinase dependent expression of GluClv2.0 alpha subunit. GluClα contains a Cerulean tag. When co-expressed with GluClv2.0 beta subunit, agonist (Ivermectin) induces neuronal silencing.
    Depositor
    David Bennett
    Article
    Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0
    Insert
    GluClα -Cerulean
    Use
    AAV and Cre/Lox
    Tags
    Cerulean
    Expression
    Mutation
    Promoter
    short CAG
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196995-plasmid-map-sequence-id-390150

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  13. pMRBad-Z-C-DiB-RM

    Plasmid
    #168477
    Purpose
    C-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain DiB-RM‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Insert
    Leucine Zipper + C-fragment of DiB-RM
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168477-plasmid-map-sequence-id-332736

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  14. pET11a-N-DiB-RM

    Plasmid
    #168476
    Purpose
    N-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Insert
    N-fragment of DiB-RM
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168476-plasmid-map-sequence-id-332734

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  15. pET11a-Z-N-DiB-RM

    Plasmid
    #168475
    Purpose
    N-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Insert
    N-fragment of DiB-RM + Leucine Zipper
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168475-plasmid-map-sequence-id-332732

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  38. pDL025_HA-rb_IgG_HC_15F11

    Plasmid
    #193622
    Purpose
    HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.
    Depositor
    Jennifer DeLuca
    Article
    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:
    Insert
    Anti_HA_15F11 rabbit heavy chain
    Use
    Tags
    signal peptide
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193622-plasmid-map-sequence-id-446783

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  39. pDL026_HA-rb_IgG_LC_15F11

    Plasmid
    #193623
    Purpose
    HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.
    Depositor
    Jennifer DeLuca
    Article
    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:
    Insert
    Anti-HA_15F11 rabbit light chain
    Use
    Tags
    signal peptide
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193623-plasmid-map-sequence-id-380458

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  40. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid
    #221002
    Purpose
    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.
    Depositor
    Mikko Taipale, Benjamin Blencowe
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    dCasRx-RBM25-IRES-BleoR
    Use
    CRISPR
    Tags
    NLS and NLS-HA
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    221002-plasmid-map-sequence-id-436162

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Showing: 1481 - 1520 of 2028 results