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pET21a-Streptavidin-Alive

Plasmid

#20860

Purpose

Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

Insert

Streptavidin-Alive

Use

Tags

His

Expression

Bacterial

Mutation

Wt gene

Promoter

Available since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

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pEF6.mCherry-TSG101

Plasmid

#38318

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

TSG101 (TSG101 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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PB-UBC-mARG3

Plasmid

#173793

Purpose

Mammalian acoustic reporter gene 1 cassette 3 on PB-UBC plasmid

Depositor

Xiaojun Lian

Article

Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

Insert

GvpJ, F, G, L, K

Use

Tags

Expression

Mammalian

Mutation

Promoter

UBC promoter

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLacO-ISce1

Plasmid

#58505

Purpose

provides 256 Lac operator repeats adjacent to the ISce1 restriction site

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Lac repeats adjacent to 6 copies of the ISce1 restriction site

Use

Tags

Expression

Bacterial

Mutation

Promoter

no promoter

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-NMHC II-C

Plasmid

#10843

Depositor

Robert Adelstein

Article

Golomb et al J Biol Chem. 2004 Jan 23. 279(4):2800-8.

Insert

NMHC II-C (Myh14 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCIG3 B

Plasmid

#132942

Purpose

MLV packaging plasmid for B-tropic MLV gag-pol expression

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

B-tropic MLV gag-pol

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-hphMX6

Plasmid

#105156

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

mCherry-hphMX6

Use

Tags

mCherry

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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12.4kbVillin-ΔATG

Plasmid

#19358

Depositor

Deborah Gumucio

Article

Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

Insert

12.4kb Villin promoter

Use

Tags

Expression

Bacterial

Mutation

No ATG - transgenes cloned downstream of the vill…

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-HA.Tetherin

Plasmid

#41068

Depositor

Paul Spearman

Article

Ali et al PLoS One. 2010 Feb 2;5(2):e9005.

Insert

Tetherin (BST2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
CFP-CaaX

Plasmid

#155232

Purpose

membane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

CFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pTetO-ISce1-TetO

Plasmid

#58506

Purpose

ISce1 restriction site is flanked by Tet operator repeats, 96 on each side

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repeats flanking ISce1 restriction site

Use

Tags

Expression

Bacterial

Mutation

Promoter

no promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only
PT2/C-fluc

Plasmid

#20203

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

C-Fluc

Use

Transposon

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1a-Bamh1

Plasmid

#203308

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with Bamh1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEF6.HA-ARRDC3

Plasmid

#38317

Depositor

Quan Lu

Article

Nabhan et al EMBO Rep. 2010 Aug;11(8):605-11. Epub 20

Insert

ARRDC3 (ARRDC3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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289 RFP/CFP

Plasmid

#83321

Purpose

fluorescent signal joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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PlayBack-CMV-Kpn1

Plasmid

#203302

Purpose

For expression of eukaryotic genes of interest with CMV promoter with Kpn1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH_blast_MCS_Nard_GFP_D50

Plasmid

#167339

Purpose

Expresses GFP fused to progerin (d50) in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

Progerin

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

50AA deletion creating Progerin instead of mature…

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pT/CMV-SV40-LgT

Plasmid

#20204

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

SV40LgT

Use

Transposon

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pOSML-Cox2-GFP

Plasmid

#203711

Purpose

Overexpression of COX2-GFP fusion

Depositor

Timothy Hla

Article

Trifan et al J Biol Chem. 1999 Nov 26;274(48):34141-7

Insert

Cox2-GFP (PTGS2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

SV40

Available since

June 19, 2024

Availability

Academic Institutions and Nonprofits only

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