165,688 results

1. pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

   Plasmid

   #169161

   Purpose

   To express nsp10-nsp14 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
   

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-HA-LMW-FGF2

   Plasmid

   #157663

   Purpose

   Expresses FGF2 in mammalian cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Fibroblast growth factor 2 (FGF2 Human)
   

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-134

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-NSP3SA11

   Plasmid

   #89170

   Purpose

   Express Rotavirus SA11 NSP3 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 NSP3
   

   Expression

   Mammalian

   Promoter

   T7 promotor

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-CyclinF

   Plasmid

   #32975

   Depositor

   Michele Pagano

   Article

   D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

   Insert

   CYCLIN F (CCNF Human)
   

   Tags

   Flag and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pcNDA-NES-PhoCl2c-mCherry

   Plasmid

   #164037

   Purpose

   Optogenetic control of protein localization by PhoCl2c

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   NES-PhoCl2c-mCherry
   

   Tags

   kozak sequence

   Expression

   Mammalian

   Promoter

   CMV promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3n1 (572)

   Plasmid

   #70727

   Purpose

   BetI/PhlF/SrpR BCD7 3-node v41-1

   Depositor

   Richard Murray

   Article

   Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

   Inserts

   srpR-ssrA
   betI-ssrA
   phlF-ssrA
   

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-VP4SA11

   Plasmid

   #89165

   Purpose

   Express Rotavirus SA11 VP4 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP4
   

   Expression

   Mammalian

   Promoter

   T7 promotor

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS161

   Plasmid

   #194484

   Purpose

   synER Cassette

   Depositor

   Ahmad Khalil

   Article

   Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

   Insert

   synER Cassette
   

   Use

   Synthetic Biology

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Halo-SspB-PPM1H

   Plasmid

   #232870

   Purpose

   Expresses SspB-PPM1H in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

   Insert

   Halo-SspB-PPM1H (PPM1H )
   

   Tags

   Halo-SspB

   Expression

   Mammalian

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pR BirA

    Plasmid

    #113640

    Purpose

    Insect cell expression vector for BirA Biotin Ligase

    Depositor

    James Moon

    Article

    Moon et al Methods Mol Biol. 2018;1799:165-181. doi

    Insert

    BirA Biotin Ligase (birA )
    

    Expression

    Insect

    Promoter

    Metallothionein

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_429

    Plasmid

    #179098

    Purpose

    BE Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e (Cas9-NG)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MCS-3_Endofin_LgBiT

    Plasmid

    #182574

    Purpose

    This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.

    Depositor

    Bradley McConnell

    Article

    Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1

    Insert

    zinc finger FYVE-type (ZFYVE16 Human)
    

    Tags

    LgBiT (Large BiT)

    Expression

    Mammalian

    Mutation

    FYVE domain Q739-K806

    Promoter

    Herpes simplex virus (HSV)

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-Pur-CAG-hM4Di-mCherry

    Plasmid

    #80947

    Purpose

    human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

    Insert

    hM4Di-mCherry
    

    Tags

    mcherry fusion

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGCDNsam-Hnf4a-IRES-GFP

    Plasmid

    #33006

    Depositor

    Atsushi Suzuki

    Article

    Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

    Insert

    hepatic nuclear factor 4 alpha (Hnf4a Mouse)
    

    Use

    Retroviral

    Available Since

    March 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. Calreticulin-SuperNova-KDEL

    Plasmid

    #131857

    Purpose

    Expresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signal

    Depositor

    Geert van den Bogaart

    Article

    Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

    Insert

    SuperNova
    

    Tags

    KDEL ER-retention signal and Signal sequence of h…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDS24

    Plasmid

    #58952

    Purpose

    Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

    Depositor

    Thomas Fox

    Article

    Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93

    Insert

    Mitochondrially coded Arg8 protein
    

    Mutation

    Coding sequence synthetic in S. cerevisiae mitoch…

    Promoter

    n/a

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCI-SEP-GluR1

    Plasmid

    #24000

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    GluR1 (Gria1 Rat)
    

    Tags

    GluR1 signal peptide (aa1-22) and SEP (supereclip…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. PRK5-FLAG-Bag6ΔUBL

    Plasmid

    #61837

    Purpose

    express N-terminally FLAG tagged human Bag6 without UBL domain

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    Bag6 (BAG6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. enOgeuIscB with ωRNA-v13

    Plasmid

    #220940

    Purpose

    CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloing

    Depositor

    Weixin Tang

    Article

    Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

    Inserts

    CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
    OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-ET

    Plasmid

    #72542

    Purpose

    expresses FLAG tagged human BRD4 ET domain (608-699 aa)

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    BRD4 ET (BRD4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET28a hMDH1v3 (+TEV)

    Plasmid

    #204229

    Purpose

    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    hMDH1v3 (MDH1 Human)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Inserted a G after ATG to maintain kozak site

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pHS1993

    Plasmid

    #239836

    Purpose

    OMEGAOff-KRK mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    DNMT3A-DNMT3L-OrufIscB-KRK-KRAB
    

    Tags

    HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Insertion of REC domain from NbaCas9-1 + E137K/E4…

    Promoter

    CMV

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt

    Plasmid

    #175181

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn FLEX iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron

    Plasmid

    #235087

    Purpose

    GFP splicing reporter with FRG1 intron

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron (FRG1 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    custom bidirectional

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLenti PGK GFP Puro (w509-5)

    Plasmid

    #19070

    Purpose

    3rd gen lentiviral eGFP expression vector, PGK promoter, Puro 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRK5-Myc-Parkin

    Plasmid

    #17612

    Depositor

    Ted Dawson

    Article

    Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

    Insert

    Parkin (PRKN Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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27. EMTB-3XGFP

    Plasmid

    #26741

    Depositor

    William Bement

    Article

    Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

    Insert

    ensconsin (MAP7 Human)
    

    Use

    Probe for microtubules

    Tags

    3x EGFP and Myc

    Expression

    Mammalian

    Mutation

    Only contains the microtubule binding-domain of e…

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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28. pEGFP-C1-SopF

    Plasmid

    #137734

    Purpose

    Expresses EGFP fused to SopF

    Depositor

    Leigh Knodler

    Insert

    SopF
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDONR223_EDNRB_WT

    Plasmid

    #81881

    Purpose

    Gateway Donor vector containing EDNRB , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EDNRB (EDNRB Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA3-2strep-FBH1

    Plasmid

    #236464

    Purpose

    transient overexpression of FBH1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Jeong et al J Cell Biol. 2013 Jan 21;200(2):141-9. d

    Insert

    FBH1 (FBXO18 Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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