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178,769 results

Showing: 11371 - 11400 of 178769 results

  1. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Sarel Fleishman
    Publication
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83917-plasmid-map-sequence-id-174928

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  8. NPY-pHTomato

    Plasmid
    #83501
    Purpose
    Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.
    Depositor
    Sebastian Barg
    Publication
    Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NPY (NPY Human)
    Tags
    pHTomato
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83501-plasmid-map-sequence-id-163242

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  11. pZA16mflon

    Plasmid
    #75439
    Purpose
    Plasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.
    Depositor
    James Collins
    Publication
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    mf-lon
    Expression
    Bacterial
    Promoter
    pBAD
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75439-plasmid-map-sequence-id-147407

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  13. MELOPS

    Plasmid
    #80151
    Purpose
    Genetically encoded melanosome-localized pH sensor.
    Depositor
    Santiago Di Pietro
    Publication
    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OCA2 (OCA2 Human)
    Tags
    mNectarine in first luminal loop
    Expression
    Mammalian
    Mutation
    nonpathologic polymorphism D257A, pore mutation V…
    Promoter
    CMV
    Available since
    Sept. 20, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  14. Hs.NRAS G12D

    Plasmid
    #83176
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway Cloning
    Mutation
    G12D
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83176-plasmid-map-sequence-id-158139

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  20. pcDNA5-FRT-TO-AID-EGFP

    Plasmid
    #80076
    Purpose
    Mammalian expression vector for C-terminal AID-EGFP tagging
    Depositor
    Andrew Holland
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AID-EGFP
    Tags
    AID and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80076-plasmid-map-sequence-id-150336

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  25. All_in_one_CRISPR/Cas9_LacZ

    Plasmid
    #74293
    Purpose
    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening
    Depositor
    Lynne Postovit
    Publication
    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    LacZ-alpha
    Cas9
    mCherry
    Use
    CRISPR
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    Lac promoter, SV40, and T7 promoter
    Available since
    May 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74293-plasmid-map-sequence-id-140421

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Showing: 11371 - 11400 of 178769 results