19,804 results

Showing: 11611 - 11640 of 19804 results

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pJ3M-Mst2

Plasmid

#12205

Depositor

Jonathan Chernoff

Article

Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

Insert

Mst2 (STK3 Human)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/TO-3xFLAG-REV7

Plasmid

#114127

Purpose

Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

REV7 (MAD2L2 Human)

Use

Genomic integration, tetracycline inducible

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2+mScarlet-C Cloning Vector

Plasmid

#128147

Purpose

To express a protein of interest fused to the C-terminus of mScarlet

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Tags

mScarlet fusion

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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SIVu

Plasmid

#81089

Purpose

Single Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCDHblast MCSNard OST-LMNAd50

Plasmid

#22662

Depositor

Tom Misteli

Article

Pegoraro et al Nat Cell Biol. 2009 Oct . 11(10):1261-7.

Insert

LaminAd50 (=Progerin)

Use

Lentiviral

Tags

Onestrep Tag

Expression

Mammalian

Mutation

50AA deletion creating Progerin instead of mature…

Available Since

Dec. 29, 2009

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP6

Plasmid

#18065

Depositor

Edward Yeh

Article

Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

Insert

SENP6 (SENP6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Promoter

U6 and CBh

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLemir9-124

Plasmid

#31779

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

miR-9/9* and miR-124 (Mir124a-2, Mir9-3 Mouse)

Use

Lentiviral

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-gate-pGK-HYG

Plasmid

#107396

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

HA

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCM351

Plasmid

#46013

Depositor

Mary Lidstrom

Article

Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

Type

Empty backbone

Use

Cre/Lox

Promoter

na

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM10-HA

Plasmid

#65107

Purpose

Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM10 (ADAM10 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 19-455

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
pY094

Plasmid

#84743

Purpose

Expresses huAsCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
GFP

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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p-dCas9-LSD1-Hygro

Plasmid

#104406

Purpose

transient expression of dCas9-LSD1 fusion protein

Depositor

Stephan Beck

Insert

LSD1 (KDM1A Human)

Use

CRISPR

Expression

Mammalian

Mutation

IRATp970A0364D (Source Bioscience) has a P405H ch…

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCSCX

Plasmid

#63918

Purpose

Expression of the sucrose utilization phenotype

Depositor

Claudia Vickers

Article

Bruschi et al Biotechnol Adv. 2012 Sep-Oct;30(5):1001-

Insert

cscAKB

Use

Bacterial cloning vector

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pBT128c

Plasmid

#138512

Purpose

P2

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

inteinC-dSpCas9

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2.5-THP-dsRed2

Plasmid

#80335

Purpose

Fluorescent Reporter for Dopaminergic Neuron Differentiation

Depositor

Kwang-Soo Kim

Article

Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

Insert

rat TH promoter (Th Rat)

Use

AAV

Promoter

rat Tyrosine hydroxylase

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-TRE3G-BCCP-PupE-IRES-BFP

Plasmid

#113405

Purpose

Express inducible BCCP-PupE in mammalian cell

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE-IRES-BFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-DsRed2

Plasmid

#112219

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

DsRed2

Use

CRISPR

Expression

Mammalian

Mutation

DsRed2 contains a silent nucleotide substitution …

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

Plasmid

#86419

Purpose

C-terminal PCR tagging module for yeast codon optimized split GFP1-10

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

GFP1-10

Expression

Yeast

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-GBP

Plasmid

#67650

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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XBP-1 1: pFLAG.XBP1u.CMV2

Plasmid

#21832

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

XBP-1 unprocessed (Xbp1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-NSP12 (SARS-CoV-2)

Plasmid

#159107

Purpose

The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

Depositor

Thomas Tuschl

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

NSP12

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLVPT-tTR-KRAB

Plasmid

#11642

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-BroccoliSamSensor#1

Plasmid

#124363

Purpose

Overexpression of circular RNA (Broccoli-based SAM sensor)

Depositor

Samie Jaffrey

Article

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-BroccoliSamSensor#1

Expression

Mammalian

Promoter

U6+27

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJJB308

Plasmid

#107699

Purpose

AtU6 Promoter-LwaCas13a-gRNA Scaffold

Depositor

Daniel Voytas

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Type

Empty backbone

Expression

Plant

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Plasmid

#158967

Purpose

Ubiquitous expression of mtON in C. elegans

Depositor

Andrew Wojtovich

Article

Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

Insert

eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Tags

GFP

Expression

Worm

Promoter

eft-3

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-GFPnls

Plasmid

#121938

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

PCP-GFPnls

Use

Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs_FLAG-NMNAT1

Plasmid

#133259

Purpose

Express FLAG-tagged NMNAT1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NMNAT1 (NMNAT1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-mFGFR1_204

Plasmid

#58745

Purpose

Expresses Opto-mFGFR1 (light-activated receptor tyrosine kinase)

Depositor

Harald Janovjak

Article

Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

Insert

Opto-mFGFR1

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
YEE-BE4max

Plasmid

#138157

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YEE)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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19,804 results