19,602 results

1. MSCV-IRES-GFP

   Plasmid

   #20672

   Purpose

   Depositor

   Tannishtha Reya

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES EGFP

   Expression

   Mammalian

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. p6XHis-T7(P266L)

   Plasmid

   #174866

   Purpose

   Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

   Depositor

   Anna Pyle

   Article

   Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

   Insert

   T7 RNA polymerase
   

   Tags

   6XHis-tag

   Expression

   Bacterial

   Mutation

   P266L

   Promoter

   lac promoter

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACRISPR

   Plasmid

   #113348

   Purpose

   A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PE6b

   Plasmid

   #207852

   Purpose

   Mammalian expression of PE6b prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6b
   

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRIS-PITChv2-dTAG-BSD (BRD4)

   Plasmid

   #91795

   Purpose

   PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   FKBP_F36V-2xHA-P2A-BSD
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-MBP-super TEV protease

   Plasmid

   #171782

   Purpose

   Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   MBP-sTEV
   

   Tags

   Arg6 tag and Internal His6 tag

   Expression

   Bacterial

   Mutation

   No internal TEV cleavage site between the MBP and…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-mCherry

   Plasmid

   #128744

   Purpose

   Expresses mCherry in mammalian cells

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.27_ARE/NRF2-SPE

   Plasmid

   #177775

   Purpose

   Luciferase reporter for ARE/NRF2

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   NRF2 (NFE2L2 Human)
   

   Use

   Luciferase

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pREP-8xARE-GFP-SV40-BFP

    Plasmid

    #134910

    Purpose

    NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Inserts

    8xARE- minimal promoter - GFP
    TagBFP
    

    Expression

    Mammalian

    Promoter

    8xARE - minimal promoter and SV40

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT3-EF1a-YapS127A

    Plasmid

    #46049

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    EF1a

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pDGM6

    Plasmid

    #110660

    Purpose

    Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.

    Depositor

    David Russell

    Article

    Gregorevic et al Nat Med. 2004 Aug;10(8):828-34. doi: 10.

    Insert

    AAV6 cap genes, AAV2 rep genes, adenovirus helper genes
    

    Use

    AAV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJM1-Tmem192-mRFP-3xHA

    Plasmid

    #134631

    Purpose

    bait for affinity-isolation of lysosomes

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    3xHA, TEV, and mRFP

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-ATLASsnCre

    Plasmid

    #232351

    Purpose

    Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells.

    Depositor

    Don Arnold

    Article

    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

    Has Service

    AAV5

    Insert

    ATLASsnCre
    

    Use

    AAV

    Tags

    ALFA-Tag

    Promoter

    hSyn

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C1-G3BP1-WT

    Plasmid

    #135997

    Purpose

    WT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCXLE-hOCT3/4-shp53-F

    Plasmid

    #27077

    Purpose

    Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    OCT3/4 (POU5F1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCXLE-hUL

    Plasmid

    #27080

    Purpose

    Integration-free (episomal) expression of human L-MYC and LIN28

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    L-MYC (LIN28A, MYCL Human)
    

    Expression

    Mammalian

    Available Since

    March 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET30ax

    Plasmid

    #85761

    Purpose

    A bacterial expression vector with a His-tag at N and C-terminus.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Type

    Empty backbone

    Tags

    His tag (N-term and C-term)

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBI-MCS-EGFP

    Plasmid

    #16542

    Depositor

    Bert Vogelstein

    Article

    Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-Sfp

    Plasmid

    #159617

    Purpose

    Expression plasmid for Sfp phosphopantetheinyl transferase

    Depositor

    Christopher T Walsh, Jun Yin

    Article

    Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

    Insert

    Sfp
    

    Tags

    6HIS

    Expression

    Bacterial

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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