We narrowed to 2,124 results for: PORA

1. pHR Grb2-TagBFP

   Plasmid

   #188631

   Purpose

   Human Grb2 C-terminally labeled with TagBFP

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   GRB2 (GRB2 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P38KTRDronpa

   Plasmid

   #205766

   Purpose

   Expression of JNK KTR rsGreenF-E, ERK KTR rsFastLime, PKA KTR Clover, and P38 KTR Dronpa in mamalian cells

   Depositor

   Edward Boyden

   Article

   Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

   Insert

   JNK KTR rsGreenF-E-2A-ERK KTR rsFastLime-IRES-PKA KTR Clover-2A-P38 KTR Dronpa
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRXIB-mRuby3-LC3

   Plasmid

   #221738

   Purpose

   Stable expression of mRuby3-LC3 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. RGB-S reporter

   Plasmid

   #207841

   Purpose

   A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli

   Depositor

   Christof Niemeyer

   Article

   Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(

   Inserts

   RpoH sensing construct
   SOS sensing construct
   RpoS sensing construct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PosmY, PsulA, and grpE

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAL113

   Plasmid

   #22471

   Purpose

   Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PhyB NT (PHYB Mouse, Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   10aaLinker-mCFP-HrasCT

   Expression

   Mammalian

   Mutation

   Y276H

   Promoter

   CMV

   Available since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2/sh-Col1a1/GFP4_Seq1.2

   Plasmid

   #201403

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIR-DsRed-IRES-His-Halo-Tev-Keap1

   Plasmid

   #58240

   Purpose

   expresses halo tagged human Keap1 protein

   Depositor

   Yimon Aye

   Article

   Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

   Insert

   Kelch like ECH associated protein 1 (KEAP1 Human)
   

   Use

   Tags

   His-Halo-Tev

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pBpaRS3tRNA

   Plasmid

   #197102

   Purpose

   To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

   Insert

   TyrRS variant, amber suppressor tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EFS-DIO-GRAB_OT1.0

   Plasmid

   #185387

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin sensor GRAB_OT1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLM-mCerulean-cMyc

    Plasmid

    #23244

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLM-mCherry-Klf4

    Plasmid

    #23243

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcBac1-Pyl-TAG

    Plasmid

    #228784

    Purpose

    Pyl aaRS/tRNA system for mammalian TAG suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    PylRS
    PytR
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

    Plasmid

    #89873

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (Please see depositor comments …

    Promoter

    Human synapsin

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti_PGK-Cas9-P2A-HygR

    Plasmid

    #164135

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAuroraA-GFP-AURKA-mCherry

    Plasmid

    #99878

    Purpose

    Expression of GFP-AURKA-mCherry in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    AURKA minimal

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAuroraA-GFP-AURKA_K162M-mCherry

    Plasmid

    #99880

    Purpose

    Expression of GFP-AURKA K162M-mCherry in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Mutation

    K162M (kinase-dead)

    Promoter

    AURKA minimal

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUGW-RUSH-LAMP2A-mNeonGreen

    Plasmid

    #228525

    Purpose

    Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP2A-mNeonGreen in mammalian cells.

    Depositor

    Ginny Farias

    Article

    Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

    Insert

    LAMP2 (Lamp2 Rat)
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP), Streptavidin-…

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti_EFS-Cas9-P2A-HygR

    Plasmid

    #164134

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

    Plasmid

    #89874

    Purpose

    expresses DRD2-iTango component in mammalian cells for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    DRD2-V2tail-TevN-BLITz1-TetR-VP16
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    short isoform of DRD2 (deletion of 242-270)

    Promoter

    Human synapsin

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRXIP-GFP-Spo20(PABD)

    Plasmid

    #221758

    Purpose

    Stable expression of GFP-Spo20(PABD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    Spo20 (SOS1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem Spo20(PABD) (amino acids 422-551)

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCR3.1-mGM-CSF

    Plasmid

    #74465

    Purpose

    expression of mGM-CSF in mammalian cells

    Depositor

    Loree Heller

    Article

    Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

    Insert

    granulocyte-monocyte colony-stimulating factor (Csf2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

    Plasmid

    #90008

    Purpose

    Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    NBirA*-linker-FKBP
    FRB-linker-CBirA*
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLV-EF1A-LivePAR-Hygro

    Plasmid

    #176063

    Purpose

    EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    LivePAR
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir

    Plasmid

    #90004

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    FKBP-linker-Myc-NBirA*
    CBirA*-linker-FRB
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

    Plasmid

    #90003

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    NBirA*-linker-FKBP
    CBirA*-linker-FRB
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ppk301-T2A-QF2 HDR plasmid

    Plasmid

    #130667

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
    

    Use

    Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir

    Plasmid

    #90009

    Purpose

    Split-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    FKBP-linker-Myc-NBirA*
    FRB-linker-CBirA*
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FKBP-epsin(144-575)-GFP

    Plasmid

    #100733

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    epn1 (Epn1 Mouse)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    clathrin binding middle part aa 144-575

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CD8-mCherry-FRB

    Plasmid

    #100738

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD8A (CD8A Human)
    

    Use

    Tags

    FRB and mCherry

    Expression

    Mammalian

    Mutation

    Truncated

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET22b-T5-sfGFP*- MmH-MH4R

    Plasmid

    #196409

    Purpose

    Express sfGFP with TAG and enzymes for biosynthesizing DOPA

    Depositor

    Han Xiao

    Article

    Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

    Inserts

    sfGFP with TAG at 151 position
    Tyrosine Hydroxylase (Th Mouse)
    dihydropterine reductase from human (QDPR Human)
    pterin-4a-carbinolamine dehydratase from human
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge