We narrowed to 7,853 results for: 11
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Plasmid#78205PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensorDepositorInsertmatB (His6 C106A R286C Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A R286C Q457C K488APromoterT7Available SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31
Plasmid#165887PurposeG418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertionsDepositorInsertAll-in-one FC31 RMCE Cassette
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSY6
Plasmid#90482PurposeTargeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicumDepositorInsertgroup II intron and ItrA
ExpressionBacterialAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT003
Plasmid#182713PurposeaTc inducible dCasRx-IF3DepositorInsertdCasRx
UseCRISPRTags3x(GGGS)-Escherichia Coli Initiation Factor 3ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpTetAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1663
Plasmid#51484PurposeminiMos transposable element with HygroR and peel-1, MCS cloningDepositorTypeEmpty backboneExpressionWormAvailable SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL70008
Plasmid#50336DepositorInsertpFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
UsePlant expressionMutationBsaI/BbsI sites removed by point-mutationAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pICH51266
Plasmid#50267DepositorInsertpromoter (1.3 kb ), 35s (Cauliflower Mosaic Virus) + 5'UTR omega (Tobacco Mosaic Virus)
UsePlant expressionMutationBsaI/BbsI sites removed by point-mutationAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
plvx-AD8
Plasmid#191558PurposeInducible expression of HIV-1 EnvDepositorInsertHIV-1 AD8 env
UseLentiviralMutationNoneAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1655
Plasmid#51481PurposeminiMos transposable element with HygroR, R4-R3 Gateway cloningDepositorTypeEmpty backboneExpressionWormAvailable SinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B
Plasmid#21682DepositorAvailable SinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-mNeon
Plasmid#186598PurposeEncodes human full-length Reticulon 2 isoform B fused to mNeonGreenDepositorAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-citrate
Plasmid#225251PurposeBacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrateDepositorInsertqmTQ2-citrate
Tags6XHis, T7 tag, XpressTM tagExpressionBacterialPromoterT7 promoterAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
cavin-3-HA
Plasmid#27711DepositorAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Alpha1
Plasmid#165862PurposeAlpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101102PurposeEncodes dCas9(C)-VP64 with SAM components fused to transmembrane tetherDepositorInsertTMt-dCas9(C)-VP64
UseCRISPR and Synthetic BiologyTagsHA and MycExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-HA-mAVO3 (pRL62-3)
Plasmid#39210DepositorAvailable SinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTet-ctl
Plasmid#136693PurposeControl for pTet-Gli2shRDepositorTypeEmpty backboneUseLentiviralAvailable SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas_2+5
Plasmid#60594Purposeencodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2DepositorInsertCas_2+5
ExpressionBacterialAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
RSRF-Luc-2mut
Plasmid#31819DepositorInsertMutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
UseLuciferaseExpressionMammalianMutationtwo CTATATTTAG RSRF sites mutated to CGATATTTCGPromotermutated MEF2-dependent (-307 to -242 of Nur77/NR4…Available SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET:GACT:GFP
Plasmid#60734PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMLS716
Plasmid#188888PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::CreDepositorInsertfloxed Cbr-unc-119 marker and Phsp16.41::Cre
ExpressionWormMutationC. elegans codom optimization and intron additionAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCXN2-mVIN
Plasmid#11776DepositorInsertvinculin (Vcl Mouse)
ExpressionMammalianAvailable SinceOct. 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-Flag-Par6 S345A
Plasmid#11749DepositorAvailable SinceAug. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL3-LRR
Plasmid#66937PurposeDestination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Reverse orientation (attR2, attR1).DepositorTypeEmpty backboneUseLuciferaseExpressionMammalianPromoterSV-40Available SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-T43NTR-TrxA-H10
Plasmid#121677PurposeBacterial expression of neurotensin receptor fusion proteinDepositorInsertneurotensin receptor (Ntsr1 Rat)
TagsTrxA-H10ExpressionBacterialMutationdeleted amino acids 1-42PromoterlacAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBR322 14-3-3 Promoter
Plasmid#16517DepositorInsert14-3-3 promoter (SFN Human)
ExpressionBacterialAvailable SinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Omega1
Plasmid#165864PurposeOmega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK-hFluc-CrPV-hRluc-6xbsMS2-Malat_AC
Plasmid#148443PurposeMammalian Expression of 6xbsMS2-MalatDepositorInsert6xbsMS2-Malat
ExpressionMammalianAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL50015
Plasmid#50318PurposePlease note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)DepositorInsertCDS, mNEON (Branchistoma lanceolatum)
UsePlant expressionMutationBsaI/BbsI sites removed by point-mutationAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-Evf1
Plasmid#99479PurposeSubcloning of the Evf1 long non-coding RNADepositorInsertEvf1
ExpressionBacterialAvailable SinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEntry Tshld(FKBP F36V)-GFP
Plasmid#53260PurposepEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.DepositorInsertTshld(FKBP F36V)-GFP
TagsEGFP, Flag, and HAMutationFKBP F36VAvailable SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
SNV-gfp-IRES-MCS
Plasmid#53122PurposeRetrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorTypeEmpty backboneUseRetroviral; For expression in chicken cellsTagsEGFP-IRESPromoterSNV (spleen necrosis virus)Available SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A human LRP6 E1E2 (20–629)
Plasmid#132627Purposeexpresses human LRP6 E1E2 (20–629) in Hi5 insect cellDepositorInsertLRP6 (20–629)
ExpressionInsectAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rap-MESA-20-M-Gal4
Plasmid#84554PurposeMESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivatorDepositorInsertRap-MESA-FRB-20-M-Gal4
UseAAVExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL70002
Plasmid#50333DepositorInsertBiotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))
UsePlant expressionMutationBsaI/BbsI sites removed by point-mutationAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMZ1001
Plasmid#204398PurposeA pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyPromoterPftsH and Synthetic promoter PRpa0002Available SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS G_tTA-BFP
Plasmid#58871PurposeMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS G_tTA-BFP
Plasmid#58856PurposeMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R626A
Plasmid#84932PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.DepositorInsertcAMPr Y205A R626A
ExpressionInsectMutationPKA-C Y205A, PKA-R R626APromoter5X UASAvailable SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICH87633
Plasmid#50271DepositorInsertpromoter, nos, (Agrobacterium tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
UsePlant expressionMutationBsaI/BbsI sites removed by point-mutationAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101107PurposeEncodes dCas9(C)-VP64 with SAM components fused to BDKRB2DepositorInsertBDKRB2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Dppa1-T2A-H2B-Halo donor
Plasmid#113118PurposeDonor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequenceDepositorInsertDppa1 donor region including homology arms and T2A-H2B-Halo
UseSynthetic BiologyAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
f2H ∂HD (272)
Plasmid#24428DepositorInsertSULF2 (SULF2 Human)
TagsFLAG, His, and MycExpressionMammalianMutationAA 418-716 deleted, 23AA N-terminal truncationAvailable SinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHygroR-GMRWhite
Plasmid#165886PurposeHygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE
Plasmid#101108PurposeEncodes dCas9(N) fused to BDKRB2DepositorInsertBDKRB2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcD-10xHis)
Plasmid#176192PurposegfcD protein expressionDepositorInsertgfcD
Tags10xHisExpressionBacterialPromoterPtacAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG26(gfcD)
Plasmid#176198PurposegfcD protein expressionDepositorInsertgfcD
Tags10xHisExpressionBacterialPromoterT7Available SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcBCD)
Plasmid#176193Purposeexpresses 3 overlapping genes (gfcB, gfcC, gfcD) from this one operonDepositorInsertgfcBCD
ExpressionBacterialPromoterPtacAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA
Plasmid#188704PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2DepositorInsertNFATC2 sgRNAs
UseLentiviralExpressionMammalianPromoterhUbCAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only