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We narrowed to 18,729 results for: MUT

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Showing: 11941 - 11970 of 18729 results

  3. AICSDP-079:DCP1A-mEGFP

    Plasmid
    #193922
    Purpose
    Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A
    Depositor
    Allen Institute for Cell Science
    Insert
    DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Dec. 22, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193922-plasmid-map-sequence-id-380539

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  4. pMLM3705

    Plasmid
    #47754
    Purpose
    Expresses mammalian cell codon-optimized dCas9-VP64
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Insert
    codon optimized dCas9-VP64
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A and H840A mutations in Cas9 (catalytically i…
    Promoter
    CMV
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47754-plasmid-map-sequence-id-68222

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  8. AICSDP-80:UBTF-HaloTag

    Plasmid
    #133963
    Purpose
    Homology arms and HaloTag-linker sequence for N-terminus tagging of human UBTF
    Depositor
    Allen Institute for Cell Science
    Insert
    UBTF Homology Arms with HaloTag-linker (UBTF Human)
    Use
    CRISPR
    Tags
    HaloTag-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    133963-plasmid-map-sequence-id-264347

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  9. pYPQAT45

    Plasmid
    #223417
    Purpose
    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for dicot plants; TTTV PAM; LbCas12a-D156R and the crRNA was driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-LbCas12a-D156R-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223417-plasmid-map-sequence-id-453515

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  10. pPT-TAR C1

    Plasmid
    #141246
    Purpose
    Transformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Algal Research, 2020. 50, 102014
    Insert
    Mitochondrial Genome of Phaeodactylum tricornutum
    Use
    Synthetic Biology; Diatom expression
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Available Since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    141246-plasmid-map-sequence-id-367010

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  14. pCDNA3-LOV-VP16

    Plasmid
    #42499
    Purpose
    LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor
    Depositor
    Ricardo Dolmetsch
    Article
    Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d
    Insert
    FKF1 (N-LOV, G128D mutant) fused with VP16
    Tags
    F-type VP16 activation domain and HA tag
    Expression
    Mammalian
    Mutation
    G128D 1-168aa
    Promoter
    CMV
    Available Since
    March 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    42499-plasmid-map-sequence-id-57336

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  16. Traptavidin-E6

    Plasmid
    #59549
    Purpose
    pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Core Traptavidin-E6
    Tags
    E6 tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pUBC_HA_2xPP7-VP64

    Plasmid
    #68418
    Purpose
    Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    PP7(deltaFG)
    Use
    Synthetic Biology
    Tags
    HA and VP64
    Expression
    Mammalian
    Mutation
    PP7: [delta]67_74 (non-aggregating)
    Promoter
    human UbC
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68418-plasmid-map-sequence-id-125550

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  20. pMBP-SpyCatcher

    Plasmid
    #79053
    Purpose
    Expresses maltose binding protein (MBP) SpyCatcher fusion
    Depositor
    James Bowie
    Article
    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi
    Insert
    MBP-SpyCatcher
    Tags
    6xHis and Maltose binding protein
    Expression
    Bacterial
    Mutation
    Derived from pDEST14-SpyCatcher (Plasmid #35044)…
    Promoter
    T7
    Available Since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    79053-plasmid-map-sequence-id-147896

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  21. AICSDP-57: SMC1A-mEGFP

    Plasmid
    #114406
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A
    Depositor
    Allen Institute for Cell Science
    Insert
    SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114406-plasmid-map-sequence-id-220833

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  27. 3XFLAG-NSarQ27G-VMAn-FKBP

    Plasmid
    #70229
    Purpose
    Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (N-terminal portion)
    Tags
    3XFLAG, FK506- and rapamycin-binding protein (FKB…
    Expression
    Mammalian
    Mutation
    Changed Gln 27 to Gly
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  28. pNAS1B

    Plasmid
    #61968
    Purpose
    Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    mCherry
    GFP
    Expression
    Bacterial
    Mutation
    Serine 65 to Glycine (GGT), Serine 72 to Alanine …
    Promoter
    PBAD and PLtetO
    Available Since
    Jan. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    61968-plasmid-map-sequence-id-101267

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  29. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid
    #115698
    Purpose
    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae
    Depositor
    Eckhard Boles
    Article
    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10
    Insert
    SSKar2-sfpHluorin-HDEL
    Tags
    ER retention signal containing HDEL and ER signal…
    Expression
    Yeast
    Mutation
    for introduced mutations see publication
    Promoter
    MET25
    Available Since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    115698-plasmid-map-sequence-id-223642

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  30. pYPQAT46

    Plasmid
    #223418
    Purpose
    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223418-plasmid-map-sequence-id-454166

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Showing: 11941 - 11970 of 18729 results