We narrowed to 9,351 results for: sacs

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pAG414GPD-dCas9-VPR

Plasmid

#63801

Purpose

pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Yeast

Promoter

GPD

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

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yeast_DualReporter

Plasmid

#127546

Purpose

A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).

Depositor

Aviv Regev

Article

de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do

Inserts

bla
mCherry
URA3 (URA3 Budding Yeast)
yEVenus
Nat1

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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-3.6kb human IFN-gamma luc

Plasmid

#17599

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter (IFNG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-3.6 kb to +70 of promoter

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-Ost1-alphaf(I)-Btl2

Plasmid

#117667

Purpose

Study secretion efficiency of Btl2

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-alphaf(I)-Btl2

Expression

Yeast

Mutation

This plasmid encodes the lipase Btl2 together wit…

Promoter

pAOX1

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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flag-hKLF6 (1006)

Plasmid

#49488

Purpose

expresses human flag tagged KLF6 in mammalian cells

Depositor

Scott Friedman

Article

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

KFL6 (KLF6 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
pFAST-BAC BAF155/SMARCC1-His

Plasmid

#177863

Purpose

Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

Depositor

Yoshihiro Izumiya

Article

Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

Insert

SMARCC1 (SMARCC1 Human)

Tags

His and His tag at C-terminus with GGGGS linker

Expression

Insect

Promoter

polyhedrin

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNAI-GAL4-CREB-S133A

Plasmid

#46770

Purpose

Expression of GAL4 fusion to CREB-S133A

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB-S133A (Creb1, GAL4 Rat, Budding Yeast)

Tags

GAL4

Expression

Mammalian

Mutation

Changed CREB serine 133 to Alanine

Promoter

CMV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNAI-GAL4-CREB-S142A

Plasmid

#46771

Purpose

Expression of GAL4 fusion to CREB-S142A

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB-S142A (Creb1, GAL4 Rat, Budding Yeast)

Tags

GAL4

Expression

Mammalian

Mutation

Changed CREB serine 142 to Alanine

Promoter

CMV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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-204 human IFN-gamma luc

Plasmid

#17596

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter (IFNG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-204 to +38 of promoter

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pFL His-p97

Plasmid

#113510

Purpose

Expression of His-p97 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97 (VCP Human)

Tags

6*His

Expression

Insect

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-hM₃O-IP

Plasmid

#46645

Depositor

Nobuaki Kikyo

Article

Hirai et al Stem Cells. 2011 Sep;29(9):1349-61. doi:

Insert

hM₃O (POU5F1 Human)

Use

Retroviral

Tags

IRES Puro and human MyoD (aa 1-62)

Expression

Mammalian

Mutation

contains a fragment of human MyoD (aa1-62) fused …

Available Since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-neo-RL-ZEB1 200bmutx5

Plasmid

#35537

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Human, Budding Yeast)

Use

Luciferase

Expression

Mammalian

Mutation

5 mutations in miR200b seed regions of 3'UTR…

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo

Plasmid

#212821

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Tags

CUbo

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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p2R3a-VenusYFP-3AT

Plasmid

#71269

Purpose

Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

VenusYFP

Use

Gateway

Tags

4xGly linker and T3A pea Pisum sativum ribulose-1…

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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FLINC-AKAR1-CAAX

Plasmid

#87705

Purpose

Fluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

FLINC-AKAR1-CAAX

Tags

C-terminal targeting sequence from Kras

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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kanMX6-ins2

Plasmid

#195039

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

KanR

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcsII-pb2-flag

Plasmid

#86766

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 2 (PSMB2 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pKrox24(MapERK)d1EGFP (KroxDs)

Plasmid

#214912

Purpose

for PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activation

Depositor

Pavel Krejčí

Article

Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

Insert

d1EGFP under the MapERK promoter

Expression

Mammalian

Promoter

MapkERK promoter

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 N C-V5-tag

Plasmid

#179987

Purpose

Mammalian expression vector for RaTG13 N, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

RaTG13 N

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pC-SUR-YR

Plasmid

#61768

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).

Depositor

Ken Haynes

Article

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

Sur gene
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

Magnaporthe oryzae ILV promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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